Abstract
An anaerobic consortium taken from brackish sediments, enriched byPCE/CH3OH sequential feeding, was capable of completely dechlorinating tetrachloroethene(PCE) to ethene (ETH). In batch experiments, PCE (0.5 mM) was dechlorinated to ethene (ETH) in approximately 75 h with either CH3OH or H2 as the electron donor. When VC (0.5 mM) was added instead of PCE it was dechlorinated without any initial lag by the PCE/CH3OHenriched consortium, although at a lower dechlorination rate. In batch tests H2 could readilyreplace CH3OH for supporting PCE dechlorination, with a similar PCE dechlorination rate andproduct distribution with respect to those observed with methanol. This indicates that H2 productionduring CH3OH fermentation was not the rate-limiting step of PCE or VC dechlorination.Acetogenesis was the predominant activity when methanol was present. A remarkable homoacetogenicactivity was also observed when hydrogen was supplied instead of methanol.
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Aulenta F, Majone M, Di Pinto AC, Tomei MC & Tandoi V. (2001) Reductive dechlorination of perchloroethene to ethene by microbial consortia in sediments. Proceedings of 9th World Congress on Anaerobic Digestion. Antwerpen, 2: 239-244
Ballapragada BS, Stensel DH, Puhakka JA & Ferguson JF (1997) Effect of hydrogen on reductive dechlorination of chlorinated ethenes. Environ. Sci. Technol. 31: 1728-1734
Berbenni P (1994) La contaminazione delle acque sotterranee da parte di composti organici ed inorganici. Inquinamento 5: 50-59
Carr CS & Hughes JB (1998) Enrichment of high-rate PCE dechlorination and comparative study of lactate, methanol, and hydrogen as electron donors to sustain activity. Environ. Sci. Technol. 32: 1817-1824
Carter SR & Jewell WJ (1993) Biotransformation of tetrachloroethylene by anaerobic attached-films at low temperature. Wat. Res. 4: 607-615
Cord-Ruwisch R & Ollivier B (1986) Interspecific hydrogen transfer during methanol degradation between Sporomusa acidovorans and hydrogenophilic anaerobes. Arch. Microbiol. 144: 163-165
De Bruin WP, Kotterman JJ, Posthumus MA, Schraa G & Zehnder AJB (1992) Complete biological transformation of tetrachloroethene to ethane. Appl. Environ. Microbiol. 58(6): 1996-2000
DiStefano TD, Gossett JM & Zinder SH (1991) Reductive dechlorination of high concentration of tetrachloroethene to ethene by an anaerobic enrichment culture in absence of methanogenesis. Appl. Environ. Microbiol. 57: 2287-2292
DiStefano TD, Gossett JM & Zinder SH (1992) Hydrogen as an electron donor for dechlorination of tetrachloroethene by an anaerobic mixed culture. Appl. Environ. Microbiol. 58: 3622-3629 424
Fennel DE, Gossett JM & Zinder SH (1997) Comparison of butyric acid, ethanol, lactic acid, and propionic acid as hydrogen donors for the reductive dechlorination of tetrachloroethene. Environ. Sci. Technol. 31: 918-926
Freedman D & Gossett JM (1989) Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions. Appl. Environ. Microbiol. 55: 2144-2151
Gao J, Skeen RS, Hooker BS & Quesenberry RD (1997) Effect of several electron donors on tetrachloroethylene dechlorination in anaerobic soil microcosms. Wat. Res. 31(10): 2479-2486
Gossett JM (1987) Measurement of Henry's law constants for C1 and C2 chorinated hydrocarbons. Environ. Sci. Technol. 21: 202-208
Haston ZC & McCarty PL (1999) Chlorinated ethene half-velocity coefficients (KS ) for reductive dehalogenation. Environ. Sci. Technol. 33: 223-226
He J, Sung Y, Dollhopf ME, Fathepure BZ, Tiedje JM & Löffler FE (2002) Acetate versus hydrogen as direct electron donors to stimulate the microbial reductive dechlorination process at chloroethene-contaminated sites. Environ. Sci. Technol. 36: 3945-3952
Komatsu T, Shinmyo J & Momonoi K (1997) Reductive transformation of tetrachloroethylene to ethylene and ethane by an anaerobic filter. Wat. Sci. Tech. 36(6-7): 125-132
Kotsyurbenko OR, Glagolev MV, Nozhevnikova AN & Conrad R (2001) Competition between homoacetogenic bacteria and methanogenic archea for hydrogen at low temperature. FEMS Microbiology Ecology 38: 153-159
Mazur CS & Jones JW (2001) Hydrogen concentration in sulphatereducing estuarine sediments during PCE dehalogenation. Environ. Sci. Technol. 35: 4783-4788
Maymò-Gatell X, Tandoi V, Gossett JM & Zinder SH (1995) Characterisation of an H2-utilizing anaerobic enrichment culture that reductively dechlorinates tetrachloroethene to vinyl chloride and ethene in complete absence of methanogenesis and acetogenesis. Appl. Environ. Microbiol. 61: 3928-3933
Maymó-Gatell X, Chien YT, Gossett JM & Zinder SH (1997) Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276: 1568-1571
Middeldorp PJM, Luijten MLGC, van de Pas BA, van Eekert MHA, Kengen SWM, Schraa G & Stams AJM (1999) Anaerobic microbial reductive dechlorination of chlorinated ethenes. Bioremediation Journal 3: 151-169
Pavlostathis SG & Giraldo-Gomez E (1991) Kinetics of anaerobic treatment. Wat. Sci. Tech. 24: 35-59
Peters V, Janssen PH & Conrad R (1998) Efficiency of hydrogen utilization during unitrophic and mixotrophic growth of Acetobacterium woodii on hydrogen and lactate in the chemostat. FEMS Microbiology Ecology 26: 317-324
Ryoo D, Shim H, Canada K, Barbieri P & Wood TK (2000) Aerobic degradation of tetrachloroethylene by toluene-o-xylene monooxygenase of Pseudomonas stuzeri OX1. Nature Biotechnology 18: 775-778
Rossetti S, Blackall LL, Majone M, Hugenholtz P, Plumb JJ & Tandoi V (2003) Kinetic and molecular characterization of an anaerobic dechlorinating microbial community. Microbiology 149: 459-469.
Smatlak CR, Gossett JM & Zinder SH (1996) Comparative kinetics of hydrogen utilization for reductive dechlorination of tetrachloroethene and methanogenesis in an anaerobic enrichment culture. Environ. Sci. Technol. 30: 2850-2858
Tandoi V, DiStefano TD, Bowser PA, Gossett JM & Zinder SH (1994) Reductive dehalogenation of chlorinated ethenes and halogenated ethanes by a high rate anaerobic enrichment culture. Environ. Sci. Technol. 28: 973-979
Vogel TM, Criddle CS & McCarty PL (1987) Transformation of halogenated aliphatic compounds. Environ. Sci. Technol. 21: 722-736
Yang Y & McCarty PL (1998) Competition for hydrogen within a chlorinated solvent dehalogenating anaerobic mixed culture. Environ. Sci. Technol. 32: 3591-3597
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Aulenta, F., Majone, M., Verbo, P. et al. Complete dechlorination of tetrachloroethene to ethene in presence of methanogenesis and acetogenesis by an anaerobic sediment microcosm. Biodegradation 13, 411–424 (2002). https://doi.org/10.1023/A:1022868712613
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DOI: https://doi.org/10.1023/A:1022868712613