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Mapping QTLs for aluminum tolerance in maize

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Abstract

Aluminum toxicity is one of the major constraints for plant development in acid soils, limiting food production in many countries. Cultivars genetically adapted to acid soils may offer an environmental compatible solution, providing a sustainable agriculture system. The aim of this work was to identify genomic regions associated with Al tolerance in maize, and to quantify the genetic effects on the phenotypic variation. A population of 168F3:4 families derived from a cross between two contrasting maize inbred lines for Al tolerance was evaluated using the NSRL and RSRL parameters in nutrient solution containing toxic level of aluminum. Variance analyses indicated that the NSRL was the most reliable phenotypicindex to measure Al tolerance in the population, being used for further QTL mapping analysis. RFLP and SSR markers were selected for bulked segregant analysis, and additional SSR markers, flanking the polymorphisms of interest, were chosen in order to saturate the putative target regions. Seven linkage groups were constructed using 17 RFLP and 34 SSR markers. Five QTLs were mapped on chromosomes 2, 6 and 8, explaining 60% of the phenotypic variation. QTL4 and marker umc043 were located on chromosomes 8and 5, close to genes encoding for enzymes involved in the organic acids synthesis pathways, a widely proposed mechanism for Al tolerance in plants. QTL2 was mapped in the same region as Alm2,also associated with Al tolerance in maize. In addition, dominant and additive effects were important in the control of this trait in maize.

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Authors and Affiliations

  1. Department of Applied Biology, College of Agriculture and Veterinarian, São Paulo State University, Jaboticabal, SP, 14870-000, Brazil

    Fernando Enrique Ninamango-Cárdenas & José Roberto Moro

  2. EMBRAPA Maize and Sorghum, PO box 151, Sete Lagoas, MG, 35701-970, Brazil

    Claudia Teixeira Guimarães, Sidney Netto Parentoni, Newton Portilho Carneiro, Maurício Antônio Lopes & Edilson Paiva

  3. Department of Genetics, College of Agriculture 'Luiz de Queiroz', University of São Paulo, PO box 83, Piracicaba, SP, 13400-970, Brazil

    Paulo Roberto Martins

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  1. Fernando Enrique Ninamango-Cárdenas
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  2. Claudia Teixeira Guimarães
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  3. Paulo Roberto Martins
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Ninamango-Cárdenas, F.E., Teixeira Guimarães, C., Martins, P.R. et al. Mapping QTLs for aluminum tolerance in maize. Euphytica 130, 223–232 (2003). https://doi.org/10.1023/A:1022867416513

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