Abstract
Nuclear factor-kappa B has been proposed to play a role in the pathogenesis of bacterial peptidoglycan–polysaccahride-induced colitis. However, its colonic expression has not been defined in detail. The primary aim of this study was to profile this expression in the rat colon. Peptigoglycan–polysaccharide was administered to the rat colon by direct intramural injections. Gross colonic injury was determined at various time points. Concomitantly, colonic nuclear factor-kappa B was measured by an electrophoretic mobility shift assay and by immunohistochemistry. Gross colonic injury and colonic nuclear factor-kappa B expression showed similar time courses following peptigoglycan–polysaccharide administration. Peak colonic injury and nuclear factor-kappa B expression were found on day 21. Nuclear factor-kappa B was mainly expressed in submucosal inflammatory cells. In conclusion, the administration of bacterial peptidoglycan–polysaccahride to the rat colon caused a chronic colitis, which was characterized by up-regulated colonic nuclear factor-kappa B.
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Sartor RB, Cromartie WJ, Powell DW, Schwab JH: Granulomatous enterocolitis induced in rats by purified bacterial cell wall fragments. Gastroenterology 89:587-595, 1985
Yamada T, Sartor RB, Marshall S, Specian RD, Grisham MB: Mucosal injury and inflammation in a model of chronic granulomatous colitis in rats. Gastroenterology 104:759-771, 1993
Fitzpatrick LR, Wang J, Le T: Caffeic acid phenethyl ester, an inhibitor of nuclear factor-kappa B, attenuates bacterial peptidoglycan—polysaccharide-induced colitis in rats. JPET 299:915-920, 20001
Aiko S, Conner EM, Fuseler JA, Grisham MB: Effects of cyclosporine or FK506 in chronic colitis. JPET 280:1075-1084, 1997
Grisham MB, De Michele SJ, Garleb KA, Specian RD:. Sulfasalazine or enteral diets containing fish oil or oligosaccharides attnuate chronic colitis in rats. Inflamm Bowel Dis 2:178-188, 1996
Herfarth HH, Boker U, Janardhanam R, Sartor RB: Subtherapeutic corticosteroids potentiate the ability of interleukin 10 to prevent chronic inflammation in rats. Gastroenterology 115:856-865, 1998
Conner EM, Brand S, Davis JM, Laroux FS, Palombella VJ, Fueseler JW, Kang DY, Wolf RE, Grisham MB: Proteasome inhibition attenuates nitric oxide synhase expression, VCAM-1 transcription and the development of chronic colitis. JPET 282:1615-1622, 1997
Fitzpatrick LR, Wang J, Le T: Gliotoxin, an inhibitor of nuclear factor-kappa B, attenuates peptidoglycan—polysaccharide-induced colitis in rats. Inflamm Bowel Dis 8:159-167, 2002
Sartor RB, Herfarth H, Van Tol EAF: Bacterial cell wall polymer-induced granulomatous inflammation. Methods 9:233-247, 1996
Sartor RB, Rath HC, Lichtman SN, Van Tol EAF: Animal models of intestinal and joint inflammation. Balliere's Clin Rheumatol 10:55-76, 1996
McCall RD, Haskill S, Zimmerman EM, Lund PK, Thompson RC, Sartor RB: Tissue interleukin 1 and interleukin-1 receptor antagonist expression in enterocolitis in resistant and susceptible rats. Gastroenterology 106:960-972, 1994
Van Tol EAF, Holt L, Li FL, Kong FM, Rippe R, Yamauchi M, Pucilowska J, Lund PK, Sartor RB: Bacterial cell wall polymers promote intestinal fibrosis by direct stimulation of fibroblasts. Am J Physiol (Gastrointest Liver Physiol) 40 G245-G255, 1999
Lee JI, Burckart GJ: Nuclear factor kappa B:important transcription factor and therapeutic target. J Clin Pharmacol 38:981-983, 1998
Yang F, De Villiers WJS, Lee EY, McClain CJ, Varilek GW: Increased nuclear factor-kappa B activation in colitis of interleukin-2-deficient mice. J Lab Clin Med 134:378-385, 1999
Fitzpatrick LR, Wang J, Le T: In vitro and in vivo effects of gliotoxin, a fungal metabolite: efficacy against dextran sodium sulfate-induced colitis in rats. Dig Dis Sci 45:2327-2336, 2000
Thiele K, Bierhaus A, Autschbach F, Hofmann M, Stremmel W, Thiele H, Ziegler R, Nawroth PP: Cell specific effects of glucocorticoid treatment on the NF-κB/IκBα system in patients with Crohn's disease. Gut 45:663-674, 1999
Marrero JA, Matkowskyj KA, Yung K, Hecht G, Benya R: Dextran sulfate sodium-induced murine colitis activates NF-κB and increases galanin-receptor expression. Am J Physiol (Gastrointest Liver Physiol) 278:G797-G804, 2000
Rogler G, Brand K, Vogl D, Page S, Hofmeister R, Andus T, Knuechel R, Baeurle PA, Scolmerich J, Gross V: Nuclear factor κB is activated in macrophages and epithelial cells of inflamed intestinal mucosa. Gastroenterology 115:357-369, 1998
Hokari R, Kato S, Matsuzaki K, Iwai A, Kawaguchi A, Nagao S, Miyahara T, Itoh K, Sekizuka E, Nagat H, Ishii H, Iizuka T, Miyasaka M, Miura S: Involvement of mucosal addressin cell adhesion moleul-1 (MadCam-1) in the pathogenesis of granulomatous colitis in rats. Clin Exp Immunol 259-265, 2001
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Fitzpatrick, L.R., Wang, J., Le, T. et al. Time Course of Colonic Nuclear Factor-Kappa B Expression During Bacterial Peptidoglycan–Polysaccharide-Induced Colitis in Rats. Dig Dis Sci 48, 775–782 (2003). https://doi.org/10.1023/A:1022853213009
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DOI: https://doi.org/10.1023/A:1022853213009