Abstract
Nuclear factor-kappa B has been proposed to play a role in the pathogenesis of bacterial peptidoglycan–polysaccahride-induced colitis. However, its colonic expression has not been defined in detail. The primary aim of this study was to profile this expression in the rat colon. Peptigoglycan–polysaccharide was administered to the rat colon by direct intramural injections. Gross colonic injury was determined at various time points. Concomitantly, colonic nuclear factor-kappa B was measured by an electrophoretic mobility shift assay and by immunohistochemistry. Gross colonic injury and colonic nuclear factor-kappa B expression showed similar time courses following peptigoglycan–polysaccharide administration. Peak colonic injury and nuclear factor-kappa B expression were found on day 21. Nuclear factor-kappa B was mainly expressed in submucosal inflammatory cells. In conclusion, the administration of bacterial peptidoglycan–polysaccahride to the rat colon caused a chronic colitis, which was characterized by up-regulated colonic nuclear factor-kappa B.
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Fitzpatrick, L.R., Wang, J., Le, T. et al. Time Course of Colonic Nuclear Factor-Kappa B Expression During Bacterial Peptidoglycan–Polysaccharide-Induced Colitis in Rats. Dig Dis Sci 48, 775–782 (2003). https://doi.org/10.1023/A:1022853213009
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DOI: https://doi.org/10.1023/A:1022853213009