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Effects of Septal Grafts on Acetylcholine Release from Rat Hippocampus After 192 IgG-Saporin Lesion

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Abstract

The cholinergic inputs to the rat hippocampus were lesioned by intraseptal injections of 192 IgG-saporin. After 15 days, fetal septal cells were grafted into the hippocampus. Thirteen months later, hippocampal acetylcholine (ACh) release was studied by microdialysis. Lesioning reduced basal ACh release (100%) to 20% of normal, which was compensated for by the graft (71%). Infusion of the serotonin uptake inhibitor citalopram (100 μM) enhanced ACh release to the same extent (% of basal release) in all rat groups. Systemic injection of 8-OH-DPAT (0.5 mg/kg, SC), an agonist of 5-HT1A receptors, caused a smaller ACh release than citalopram. Acetylcholinesterase (AChE) staining and densitometric quantification revealed that the lesion-induced reduction of the AChE-staining density was compensated for by septal grafting. In conclusion, both histochemical and biochemical methods showed that cholinergic hippocampal parameters were drastically impaired by 192 IgG-saporin lesions, but were almost completely restored by septal grafting. The graft responded to intrinsic serotonergic regulation.

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Author notes

  1. Jochen Klein

    Present address: LN2C, UMR 7521 Université Louis Pasteur/CNRS, IFR 037 de Neurosciences, 12 rue Goethe, F-67000, Strasbourg, France

Authors and Affiliations

  1. Department of Pharmacology, University of Mainz, Obere Zahlbacher Str. 67, D-55101, Mainz, Germany

    Michael Hilgert, Joachim Hartmann, Konrad Löffelholz & Jochen Klein

  2. LN2C, UMR 7521 Université Louis Pasteur/CNRS, IFR 037 de Neurosciences, 12 rue Goethe, F-67000, Strasbourg, France

    Hélène Jeltsch & Jean-Christophe Cassel

Authors
  1. Michael Hilgert
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  2. Joachim Hartmann
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  3. Konrad Löffelholz
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  4. Hélène Jeltsch
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  5. Jean-Christophe Cassel
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  6. Jochen Klein
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Correspondence to Konrad Löffelholz.

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Hilgert, M., Hartmann, J., Löffelholz, K. et al. Effects of Septal Grafts on Acetylcholine Release from Rat Hippocampus After 192 IgG-Saporin Lesion. Neurochem Res 28, 467–472 (2003). https://doi.org/10.1023/A:1022852819018

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