Abstract
The effect phosphinothricin (PPT), an inhibitor of glutamine synthetase (GS), on proline accumulation in detached rice leaves was investigated. During 12 h incubation, PPT inhibited GS activity and induced accumulation of NH4 +, and accumulation of proline in the light but not in darkness. Proline accumulation caused by PPT in the light was related to protein hydrolysis, and increase in the contents of precursors of proline, ornithine and arginine. Abscisic acid accumulation was not required for proline accumulation in PPT-treated rice leaves.
References
Aspinall, D., Paleg, L.G.: Proline accumulation: physiological aspects.-In: Paleg, L.G., Aspinall, D. (ed.): The Physiology and Biochemistry of Drought Resistance in Plants. Pp. 280–295. Academic Press, Sydney 1981.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Brown, D.H., Fowden, L.: Metabolism of δ-acetylornithine in two leguminous species.-Phytochemistry 5: 887–892, 1966.
Chen, C.T., Chen, L.M., Lin, C.C., Kao, C.H.: Regulation of proline accumulation in detached rice leaves exposed to excess copper.-Plant Soil 160: 283–290, 2001.
Delauney, A.J., Verma, D.P.S.: Proline biosynthesis and osmoregulation in plants.-Plant J. 4: 215–223, 1993.
Downs, C.G., Christey, M.C., Maddocks, D., Seelye, J.F., Stevenson, D.G.: Hairy roots of Brassica napus: I. Applied glutamine overcomes the effect of phosphinothricin treatment.-Plant Cell Rep. 14: 37–40, 1994.
Fedina, I.S., Georgieva, K., Grigorova, I.: Light-dark changes in proline content of barley leaves under salt stress.-Biol. Plant. 45: 59–63, 2002.
Hurst, P.L., King, G.A., Borst, W.M.: Postharvest inhibition of glutamine synthetase activity with phosphinothricin reduces the shelf-life of asparagus.-Postharvest Biol. Technol. 3: 327–334, 1993.
Lea, P.J., Joy, K.W., Ramos, J.P., Guerrero, M.G.: The action of 2–amino-4–(methylphosphinyl)-butanoic acid (phosphinothricin) and its 2–oxo-derivative on the metabolism of cyanobacteria and higher plants.-Phytochemistry 23: 1–6, 1984.
Leason, M.D., Cunliffe, D., Parkin, D., Lea, P.J., Miflin, B.J.: Inhibition of pea leaf glutamine synthetase by methionine sulfoximine, phosphinothricin and other glutamate analogue. — Phosphinothricin and other glutamate analogue.-Phytochemistry 21: 855–857, 1982.
Lin, C.C., Kao, C.H.: Regulation of ammonium-induced proline accumulation in detached rice leaves.-Plant Growth Regul. 35: 69–74, 2001.
Lin, J.-N., Kao, C.H.: Water stress, ammonium, and leaf senescence in detached rice leaves.-Plant Growth Regul. 26: 165–169, 1998.
Lin, J.-N., Wang, J.-W., Kao, C.H.: Effect of abscisic acid and water stress on the senescence of detached rice leaves.-Biol. Plant. 42: 313–316, 1999.
Lingnowski, E.M., Splittstoesser, W.E.: The change in arginine levels and metabolism of urea and ornithine in Cucurbita moschatta seedlings.-Physiol. Plant. 25: 225–229, 1971.
McDonell, E.M., Coughlan, S.J., Wyn Jones, R.G.: Differential effects of abscisic acid on glycinebetaine and proline accumulation in three plant species.-Z. Pflanzenphysiol. 109: 207–213, 1983.
Miflin, B.J., Lea, P.J.: The pathway of nitrogen assimilation in plants.-Phytochemistry 15: 873–885, 1976.
Palmer, C.E., Oelck, M.: The relationship of phosphinothricin to growth and metabolism in cell cultures of Brassica napus L.-J. Plant Physiol. 141: 105–110, 1992.
Perez-Garcia, A., de Vicente, A., Canton, F.R., Cazorla, F.M., Codina, J.C., Garcia-Gutierrez, A., Canovas, F.M.: Light-dependent changes of tomato glutamine synthetase in response to Pseudomonas syringae infection or phosphinothricin treatment.-Physiol. Plant. 102: 377–384, 1998.
Sauer, H., Wild, A., Ruhle, W.: The effects of phosphinothricin (gluphosinate) on photosynthesis II. The causes of inhibition of photosynthesis.-Z. Naturforsch. 42c: 270–278, 1987.
Tachibana, K., Watanabe, T., Seikizawa, Y., Takematsu, T.: Accumulation of ammonium in plants treated with bialaphos.-J. Pestic. Sci. 11: 33–37, 1986.
Tsai, Y.-C., Chen, C.T., Hsu, Y.T., Kao, C.H.: The toxicity induced by phosphinothricin and methionine sulfoximine in rice leaves is mediated through ethylene but not abscisic acid.-Plant Growth Regul., in press, 2002.
Tsai, Y.-C., Kao, C.H.: Light-dependent ammonium ion toxicity of rice leaves in response to phosphinothricin.-Biol. Plant. 45: 569–573, 2002.
Wendler, C., Barniske, M., Wild, A.: Effect of phosphinothricin (glufosinate) on photosynthesis and photorespiration of C3 and C4 plants.-Photosynth. Res. 24: 55–61, 1990.
Wendler, C., Wild, A.: Effect of phosphinothricin on photosynthesis and photorespiration.-Z. Naturforsch. 45c: 535–537, 1990.
Wild, A., Sauer, H., Ruhle, W.: The effect of phosphinothricin (glufosinate) on photosynthesis. I. Inhibition of photosynthesis and accumulation of ammonia.-Z. Naturforsch. 42c: 263–269, 1987.
Yang, C.W., Kao, C.H.: Ammonium in relation to proline accumulation in detached rice leaves.-Plant Growth Regul. 30: 139–144, 2000.
Yoshiba, Y., Kiyosue, T., Nakashima, K., Yamagueni-Shinozaki, K., Shinozaki, K.: Regulation of levels of proline as an osmolyte in plants under water stress.-Plant Cell Physiol. 38: 1095–1107, 1997.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tsai, YC., Hsu, Y. & Kao, C. Proline Accumulation Induced by Phosphinothricin in Rice Leaves. Biologia Plantarum 46, 317–319 (2003). https://doi.org/10.1023/A:1022835618494
Issue Date:
DOI: https://doi.org/10.1023/A:1022835618494