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Early Postdenervation Depolarization Is Controlled by Acetylcholine and Glutamate via Nitric Oxide Regulation of the Chloride Transporter

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Abstract

Resting non-quantal acetylcholine (ACh) and probably glutamate (Glu) release from nerve endings activates M1- and NMDA receptor-mediated Ca2+ entry into the sarcoplasm with following activation of NOS and production of NO. This is a trophic message from motoneurons, which keeps the Cl− transport inactive in the innervated sarcolemma. After denervation, the secretion of ACh and Glu at the neuromuscular junction is eliminated within 3–4 h and the production of NO in the sarcoplasm is lowered. As a result, the Cl− influx is probably activated by dephosphorylation of the Cl− transporter with subsequent elevation of intracellular Cl− concentration. The equilibrium Cl− potential becomes more positive and the muscle membrane becomes depolarized.

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  1. Institute of Physiology, Academy of Sciences of the Czech Republic, Videňská 1083, Prague

    František Vyskočil

  2. Department of Animal Physiology and Developmental Biology, Faculty of Sciences, Charles University, Prague, 2, Czech Republic

    František Vyskočil

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Vyskočil, F. Early Postdenervation Depolarization Is Controlled by Acetylcholine and Glutamate via Nitric Oxide Regulation of the Chloride Transporter. Neurochem Res 28, 575–585 (2003). https://doi.org/10.1023/A:1022833709448

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