Abstract
Expression of alcohol dehydrogenase loci was used to estimate hybridity of Lycopersicon esculentum Mill. in 1012 seeds. The banding patterns were obtained by means of vertical block electrophoresis in polyacrylamide gels. It was established that qualitative variation of locus Adh-2 can be applied to prove hybridity of F1 tomato seeds. This genetic marker is indicative for hybrids with fertile maternal line or with position male sterility line and not only for maternal line with pollen male sterility.
References
Henn, G., Neitz, A.W.H., Louw, A.I.: Identification of tomato cultivars (Lycopersicon esculentum) by polyacrylamide isoelectric focusing.-Euphytica 62: 77–82, 1992.
Markova, M., Danailov, Zh., Vodenicharova, M.: Alcohol dehydrogenase loci of sterile and fertile lines of tomato in process of ontogenesis.-Acta Physiol. Plant. 3: 288–290, 2000.
Peacock, A., Bunting, S., Queen, R.: Serum protein electrophoresis on acrylamide gel patterns from normal human subjects.-Science 147: 1451–1453, 1965.
Philouze, J.: Utilisation pratique d'une lignée male-sterile ms-35 chez le tomate.-Ann. Amelior. Plant. 24: 129–144, 1974.
Qi, X.Q., Xu, G., Guo, J.Z., Yond, G.M., Zhang, B.X.: Seed purity test of Zhongjiao 6 using L-amylase isoenzyme analysis.-China Veg. 2: 9–10, 1994.
Rychter, A., Lewak, St.: Polyacrylamide gel electrophoresis of apple seed enzymes.-Acta biochem. pol. 4: 333–338, 1969.
Shaw, C., Prassad, R.: Starch gel electrophoresis of enzymes — a compilation of recipes.-Biochem. Genet. 4: 297–320, 1970.
Tanksley, S., Jones, R.: Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato.-J. hort. Sci. 16: 179–181, 1981.
Tanksley, S.D.: Linkage, chromosome association, and expression of Adh-1 and Pgm-2 in tomato.-Biochem. Genet. 17: 1159–1167, 1979.
Tanksley, S.D., Rick, C.M.: Isozymic gene linkage map of the tomato: applications in genetics and breeding.-Theor. appl. Genet. 57: 161–170, 1980.
Van den Berg, B.: A rapid and economical method for hybrid purity testing of tomato (Lycopersicon esculentum L.) F1 hybrids using ultrathin-layer isoelectric focusing of alcohol dehydrogenase variants from seeds.-Electrophoresis 12: 64–69, 1991.
Vodenicharova, M., Stoilova, Ts., Markova, M.: Comparative study of esterase and alcohol dehydrogenase loci for tomato hybridity.-Seed Sci. Technol. 24: 185–189, 1996.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Markova, M., Stoilova, T. & Danailov, Z. Application of Alcohol Dehydrogenase Loci in Testing F1 Hybridity of Tomato. Biologia Plantarum 46, 309–311 (2003). https://doi.org/10.1023/A:1022831517586
Issue Date:
DOI: https://doi.org/10.1023/A:1022831517586