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Temperature Effect on Proximal to Distal Gradient of Quantal Release of Acetylcholine at Frog Endplate

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Abstract

The conduction velocity of the nerve terminal, mean quantal content, and release latencies of uniquantal endplate currents (EPCs) were recorded in proximal, central, and distal parts of the terminal by extracellular pipettes located 5, 50, and 100 mm from the end of myelinated nerve trunk. The spike conduction velocity, minimal latency, modal value of the latency histograms, and time interval during which 90% of EPCs released (P90) at distal, central, and proximal part of the frog nerve terminal have different temperature dependency between 10° and 28°C. As shown by the size and time-course of reconstructed multiquantal EPCs, the secretion synchronization, which is greatest in distal parts, compensates at least partly for the progressive slowing of spike conduction velocity in the proximodistal direction, in particular at lower temperatures.

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, Kazan, Russian Federation

    D. Samigullin, E. Bukharaeva & E. Nikolsky

  2. State Medical University, Butlerov st. 49, Kazan, Russian Federation

    E. Bukharaeva & E. Nikolsky

  3. Department of Animal Physiology and Developmental Biology, Faculty of Sciences, Charles University, Viničná 7, Prague, 2, Czech Republic

    F. Vyskočil

  4. Institute of Physiology, Academy of Sciences of the Czech Republic, Videňská 1083, Prague, 4, Czech Republic

    F. Vyskočil

Authors
  1. D. Samigullin
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  2. E. Bukharaeva
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  3. E. Nikolsky
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  4. F. Vyskočil
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Samigullin, D., Bukharaeva, E., Nikolsky, E. et al. Temperature Effect on Proximal to Distal Gradient of Quantal Release of Acetylcholine at Frog Endplate. Neurochem Res 28, 507–514 (2003). https://doi.org/10.1023/A:1022817205814

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