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Induction of doubled haploids in sweet cherry (Prunus avium L.)

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Abstract

We report, for the first time, there generation of four homozygous lines in sweet cherry (Prunus avium L.) byin situ parthenogenesis followed by embryo and cotyledon culture. The sweet cherry cultivar ‘Altenburger’ was pollinated with marked pollen irradiated by γ-rays at doses ranging from 250 to1200 Gy. Pollination with such irradiated pollen affected fruit set and the quality of the embryos, and induced the formation of parthenogenic embryos. The immature embryos extracted from the stones, 35 or 75days after pollination, were cultivatedin vitro in an embryo or cotyledon culture. Although flow cytometrical analysis demonstrated the diploid level for all regenerants, four lines could be characterized as homozygous using isoenzyme analysis.

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Authors and Affiliations

  1. Federal Centre of Breeding Research on Cultivated Plants, Institute of Fruit Breeding, Pillnitzer Platz 3a, D-01326, Dresden, Germany

    M. Höfer & Ch. Grafe

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  1. M. Höfer
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  2. Ch. Grafe
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Höfer, M., Grafe, C. Induction of doubled haploids in sweet cherry (Prunus avium L.). Euphytica 130, 191–197 (2003). https://doi.org/10.1023/A:1022815415604

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