Abstract
Kinetics of photolysis of the antibiotic tetracycline (TC) during irradiation at 365 nm was studied in three buffer solutions usually used for studies on TC binding to its main cell targets—a transcriptional repressor protein TetR and to the ribosome. These buffer solutions contain magnesium ions and an antioxidant—mercaptoethanol or dithiothreitol. The rate of TC photolysis was maximal in medium which contained 14 mM mercaptoethanol and 5 mM magnesium ions. In the absence of mercaptoethanol the photolysis rate was more than twofold decreased. The rate constants and quantum yields of the photolysis were determined under various conditions.
Similar content being viewed by others
REFERENCES
Hillen, W., and Berens, C. (1994) Annu. Rev. Microbiol., 48, 345-369.
Roberts, M. C. (1996) FEMS Microbiol. Rev., 19, 1-24.
Oehler, R., Polacek, N., Steiner, G., and Barta, A. (1997) Nucleic Acids Res., 25(6), 1219-1224.
Goldman, R. A., Hasan, T., Hall, C. C., Strycharz, W. A., and Cooperman, B. S. (1983) Biochemistry, 22, 359-368.
Goldman, R. A., Cooperman, B. S., Strycharz, W. A., Williams, B. A., and Tritton, T. R. (1980) FEBS Lett., 118, 113-118.
Kimura, T., and Yamaguchi, A. (1996) FEBS Lett., 388, 50-52.
Beliakova, M. M., Anokhina, M. M., Spiridonova, V. A., Dobrov, E. N., Egorov, T. A., Wittmann-Liebold, B., Orth, P., Saenger, W., and Kopylov, A. M. (2000) FEBS Lett., 477, 263-267.
Blank, H., Cullen, S. I., and Catalano, P. M. (1968) Arch. Dermatol., 97, 1-2.
Agrawal, R. K., Penczek, P., Grassucci, R. A., Burkhardt, N., Nierhaus, K. H., and Frank, J. (1999) J. Biol. Chem., 274(13), 8723-8729.
Hillen, W., Klock, G., Kaffenberger, I., Wray, L. V., and Reznikoff, W. S. (1982) J. Biol. Chem., 257, 6605-6613.
Dobrov, E. N., and Nikogosyan, D. N. (1998) Photochem. Photobiol., 67(3), 269-275.
Gerold, M. (1966) Antibiotics [in Russian], Meditsina, Moscow, pp. 291-292.
Streltsov, S. A., Kukhanova, M. K., Gurskii, G. V., Kraevskii, A. A., Belyavskaya, I. V., Viktorova, L. S., Treboganov, A. D., and Gottikh, B. P. (1975) Mol. Biol. (Moscow), 9, 910-921.
Hasan, T., Allen, M., and Cooperman, B. S. (1985) J. Org. Chem., 50, 1755-1757.
Hasan, T., Kochevar, I. E., McAuliffe, D. J., Cooperman, B. S., and Abdulah, D. (1984) J. Invest. Dermatol., 83, 179-183.
Cooperman, B. S., Jaynes, E. N., Brunswick, D. J., and Luddy, M. A. (1975) Proc. Natl. Acad. Sci. USA, 72, 2974-2978.
Wang, S. Y. (1962) Photochem. Photobiol., 1, 135-145.
Rahn, R. O., and Selin, H. G. (1982) Photochem. Photobiol., 35, 459-465.
Favre, A. (1993) in Bioorganic Photochemistry (Morrison, H., ed.) Vol. 1, John Wiley & Sons, New York, pp. 380-425.
Li Anson, S. W., Chingell, R. D., and Hall, R. D. (1987) Photochem. Photobiol., 46, 379-382.
Akerman, B., and Tuite, E. (1996) Nucleic Acids Res., 24, 1080-1090.
Hlavka, J. J., and Bitha, P. (1966) Tetrahedron Lett., 32, 3843-3846.
Davies, A. K., McKellar, J. F., Phillips, G. O., and Reid, A. G. (1979) J. Chem. Soc. Perkin Trans., 2, 369-375.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Beliakova, M.M., Bessonov, S.I., Sergeyev, B.M. et al. Rate of Tetracycline Photolysis during Irradiation at 365 nm. Biochemistry (Moscow) 68, 182–187 (2003). https://doi.org/10.1023/A:1022697312042
Issue Date:
DOI: https://doi.org/10.1023/A:1022697312042