Abstract
We checked the hypothesis that the transient presence of anthocyanins in young leaves serves a photoprotective function. For this purpose, Rosa sp. and Ricinus communis L., whose young leaves are red to become green upon maturation, were used. Thus, young leaves with high and mature leaves with low anthocyanin contents were analysed concerning their carotenoid (Car) composition and susceptibility to photoinhibition. Cars, including the components of the xanthophyll cycle, had similar contents in young and mature leaves, when expressed on a chlorophyll basis. Yet, when expressed on a leaf area basis or on the assumed photon absorptive capacity of leaves, Cars contents were considerably lower in anthocyanic young leaves. Although this may indicate a low photodissipative potential, red young leaves were considerably less susceptible to photoinhibitory damage. The results are compatible with a photoprotective function of anthocyanins, indicating also that their presence may compensate for a low capacity in the xanthophyll cycle-dependent harmless dissipation of excess excitation energy.
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Abadía, J., Morales, F., Abadía, A.: Photosystem II efficiency in low chlorophyll, iron-deficient leaves.-Plant Soil 215: 183-192, 1999.
Adams, W.W., III, Demmig-Adams, B.: Carotenoid composition and down regulation of photosystem II in three conifer species during the winter.-Physiol. Plant. 92: 451-458, 1994.
Barker, D.H., Seaton, G.G.R., Robinson, S.A.: Internal and external photoprotection in developing leaves of the CAM plant Cotyledon orbiculata.-Plant Cell Environ. 20: 617-624, 1997.
Bisba, A., Petropoulou, Y., Manetas, Y.: The transiently pubescent young leaves of plane (Platanus orientalis L.) are deficient in photodissipative capacity.-Physiol. Plant. 101: 373-378, 1997.
Burger, J., Edwards, G.E.: Photosynthetic efficiency, and photodamage by UV and visible radiation, in red versus green leaf Coleus varieties.-Plant Cell Physiol. 37: 395-399, 1996.
Chalker-Scott, L.: Environmental significance of anthocyanins in plant stress responses.-Photochem. Photobiol. 70: 1-9, 1999.
Choudhury, N.K., Behera, R.K.: Photoinhibition of photosynthesis: Role of carotenoids in photoprotection of chloroplast constituents.-Photosynthetica 39: 481-488, 2001.
Demmig-Adams, B., Adams, W.W., III, Logan, B.A., Verhoeven, A.S.: Xanthophyll cycle-dependent energy dissipation and flexible photosystem II efficiency in plants acclimated to light stress.-Aust. J. Plant Physiol. 22: 249-260, 1995.
Demmig-Adams, B., Gilmore, A.M., Adams, W.W., III: In vivo functions of carotenoids in higher plants.-FASEB 10: 403-412, 1996.
Dodd, I.C., Critchley, C., Woodall, G.S., Stewart, G.R.: Photoinhibition in differently colored juvenile leaves of Syzygium species.-J. exp. Bot. 49: 1437-1445, 1998.
Feild, T.S., Lee, D.W., Holbrook, N.M.: Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood.-Plant Physiol. 127: 566-574, 2001.
Gould, K.S., Markham, K.R., Smith, R.H., Goris, J.J.: Functional role of anthocyanins in the leaves of Quintinia serrata A. Cunn.-J. exp. Bot. 51: 1107-1115, 2000.
Harborne, J.B.: Function of flavonoids in plants.-In: Goodwin, T.W. (ed.): Chemistry and Biochemistry of Plant Pigments. Pp. 736-778. Academic Press, London 1976.
Hoch, W.A., Zeldin, E.L., McCown, B.H.: Physiological significance of anthocyanins during autumnal leaf senescence.-Tree Physiol. 21: 1-8, 2001.
Ireland, C.R., Baker, N.R., Long, S.P.: The role of carbon dioxide and oxygen in determining chlorophyll fluorescence quenching during leaf development.-Planta 165: 477-485, 1985.
Ishida, A., Nakano, T., Uemura, A., Yamashita, N., Tanabe, H., Koike, N.: Light-use properties in two sun-adapted shrubs with contrasting canopy structures.-Tree Physiol. 21: 497-504, 2001.
Krause, G.H., Virgo, A., Winter, K.: High susceptibility to photoinhibition of young leaves of tropical forest trees.-Planta 197: 583-591, 1995.
Krol, M., Gray, G.R., Hurry, V.M., Öquist, G., Malek, L., Huner, N.P.A.: Low-temperature stress and photoperiod affect an increased tolerance to photoinhibition in Pinus banksiana seedlings.-Can. J. Bot. 73: 1119-1127, 1995.
Kyparissis, A., Petropoulou, Y., Manetas, Y.: Summer survival of leaves in a soft-leaved shrub (Phlomis fruticosa L., Labiatae) under Mediterranean field conditions: avoidance of photoinhibitory damage through decreased chlorophyll contents.-J. exp. Bot. 46: 1825-1831, 1995.
Lichtenthaler, H.K., Wellburn, A.R.: Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 11: 591-592, 1983.
Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994.
Mancinelli, A.L., Yang, C.-Ph., Lindquist, P., Anderson, O.R., Rabino, I.: Photocontrol of anthocyanin synthesis. III. The action of streptomycin on the synthesis of chlorophyll and anthocyanin.-Plant Physiol. 55: 251-257, 1975.
Manetas, Y., Grammatikopoulos, G., Kyparissis, A.: The use of the portable, non-destructive, SPAD-502 (Minolta) chlorophyll meter with leaves of varying trichome density and anthocyanin content.-J. Plant Physiol. 153: 513-516, 1998.
Mendez, M., Gwynn-Jones, D., Manetas, Y.: Enhanced UV-B radiation under field conditions increases anthocyanin and reduces the risk of photoinhibition but does not affect growth in the carnivorous plant Pinguicula vulgaris.-New Phytol. 144: 275-282, 1999.
Merzlyak, M.N., Chivkunova, O.B.: Light-stress induced pigment changes and evidence for anthocyanin photo-protection in apples.-J. Photochem. Photobiol. B 55: 155-163, 2000.
Miranda, V., Baker, N.R., Long, S.P.: Limitations of photosynthesis in different regions of the Zea mays leaf.-New Phytol. 89: 179-190, 1981.
Murray, J.R., Hackett, W.P.: Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L.-Plant Physiol. 97: 343-351, 1991.
Neill, S., Gould, K.S.: Optical properties of leaves in relation to anthocyanin concentration and distribution.-Can. J. Bot. 77: 1777-1782, 1999.
Šesták, Z., Tichá, I., Čatský, J., Solárová, J., Pospíšilová, J., Hodáňová, D.: Integration of photosynthetic characteristics during leaf development.-In: Šesták, Z. (ed.): Photosynthesis During Leaf Development. Pp. 263-286. Academia, Praha; Dr W. Junk Publ., Dordrecht-Boston-Lancaster 1985.
Smillie, R.M., Hetherington, S.E.: Photoabatement by anthocyanin shields photosynthetic systems from light stress.-Photosynthetica 36: 451-463, 1999.
Smirnoff, N.: The role of active oxygen in the response of plants to water deficit and desiccation.-New Phytol. 125: 27-58, 1993.
Swain, T.: Flavonoids.-In: Goodwin, T.W. (ed.): Chemistry and Biochemistry of Plant Pigments. Pp. 166-205. Academic Press, London 1976.
Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC.-Photosynth. Res. 23: 331-343, 1990.
Vogelmann, T.C.: Plant tissue optics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 44: 231-251, 1993.
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Manetas, Y., Drinia, A. & Petropoulou, Y. High Contents of Anthocyanins in Young Leaves are Correlated with Low Pools of Xanthophyll Cycle Components and Low Risk of Photoinhibition. Photosynthetica 40, 349–354 (2002). https://doi.org/10.1023/A:1022614722629
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DOI: https://doi.org/10.1023/A:1022614722629