Advertisement

World Journal of Microbiology and Biotechnology

, Volume 19, Issue 1, pp 101–105 | Cite as

Production of acetic acid by Dekkera/Brettanomyces yeasts under conditions of constant pH

  • S.N. Freer
  • B. Dien
  • S. Matsuda
Article

Abstract

Sixty yeast strains were previously screened for their ability to produce acetic acid, in shaken flask batch culture, from either glucose or ethanol. Seven of the strains belonging to the Brettanomyces and Dekkera genera, from the ARS Culture Collection, Peoria, IL, were further evaluated for acetic acid production in bioreactor batch culture at 28 °C, constant aeration (0.75 v/v/m) and pH (6.5). The medium contained either 100 g glucose/l or 35 g ethanol/l as the carbon/energy source. Dekkera intermedia NRRL YB-4553 produced 42.8 and 14.9 g acetic acid/l from the two carbon sources, respectively, after 64.5 h. The optimal pH was determined to be 5.5. When the initial glucose concentration was 150 or 200 g/l, the yeast produced 57.5 and 65.1 g acetic acid/l, respectively.

Acetic acid bioreactor constant pH Dekkera 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Borden, J.R., Lee, Y.Y. & Yoon, H-H. 2000 Simultaneous sacchari-fication and fermentation of cellulosic biomass to acetic acid. Applied Biochemistry and Biotechnology 84-86, 963-970.Google Scholar
  2. Chukwu, U.N. & Cheryan, M. 1999 Electrodialysis of acetate fermentation broths. Applied Biochemistry and Biotechnology 77-79, 485-499.Google Scholar
  3. Dunn, S.A. & Schenk, R.U. 1980 Alternate highway deicing chemicals. Federal Highway Administration Report FHWA-RD-79-106.Google Scholar
  4. Freer, S.N. 2002 Acetic acid production by Dekkera/Brettanomyces yeasts. World Journal of Microbiology and Biotechnology 18, 271-275.Google Scholar
  5. Han, I.S. & Cheryan, M. 1996 Downstream processing of acetate fermentation broths by nanofiltration. Applied Biochemistry and Biotechnology 57/58, 19-28.Google Scholar
  6. Levendis, Y.A. 1991 Catalysis of the combustion of carbonaceous particles (synthetic chars and coal) by addition of calcium acetate. In Calcium Magnesium Acetate, An Emerging Bulk Chemical for Environmental Applications, eds. Wise, D., Levendis, Y. & Metghalchi, M. pp. 221-246. Elsevier: Amsterdam. ISBN 0444885110.Google Scholar
  7. Perekh, S.R. & Cheryan, M. 1990a Acetate Production from glucose by Clostridium thermoaceticum. Process Biochemistry 25, 117-121.Google Scholar
  8. Perekh, S.R. & Cheryan, M. 1990b Fed-batch fermentation of glucose to acetate by an improved strain of Clostridium thermoaceticum. Biotechnology Letters 12, 861-864.Google Scholar
  9. Perekh, S.R. & Cheryan, M. 1991 Production of acetate by mutant strains of Clostridium thermoaceticum. Applied Microbiology and Biotechnology 36, 384-387.Google Scholar
  10. Parekh, S.R. & Cheryan, M. 1994 High concentrations of acetate with a mutant strain of C.thermoacetic um. Biotechnology Letters 16, 139-142.Google Scholar
  11. Reed, W.M., Keller, F.A., Kite, F.E., Bogdan, M.E. & Ganoung, J.S. 1986 Development of increased acetic acid tolerance in anaerobic homoacetogens through induced mauagenesis and continuous selection. Enzyme and Microbial Technology 9, 117-120.Google Scholar
  12. Sharma, P.K. 1991 Calcium impregnation of coals as a means for sulfur emissions control in combustion, In Calcium Magnesium Acetate, An Emerging Bulk Chemical for Environmental Applications, eds. Wise, D., Levendis, Y. & Metghalchi, M. pp. 273-296. Elsevier: Amsterdam. ISBN 0444885110.Google Scholar
  13. Smith, M.T., Yamazake, M. & Poot, G.A. 1990 Dekkera, Brettanomyces and Eeniella: electrophoretic comparison of enzymes and DNA-DNA homology. Yeast 6, 299-310.Google Scholar
  14. Sponholz, W-R. 1993 Wine spoilage by microorganisms. In Wine Microbiology and Biotechnology. ed. Fleet, G.H. pp. 395-420. Harwood Academic Publishers: Switzerland. ISBN 3718651327.Google Scholar
  15. Tullo, A. 1999 Sterling and BP Amico expand their capacity for acetic acid in Texas. Chemical Market Reporter. 22 February, 1999.Google Scholar
  16. Witjitra, K., Shah, M.M. & Cheryan, M. 1996 Effect of nutrient sources on growth and acetate production by Clostridium thermoaceticum. Enzyme and Microbial Technology 19, 322-327.Google Scholar
  17. Yang, S-T., Jin, Z. & Chollar, B.H. 1997 Production of low-cost acetate deicers from biomass and industrial wastes. In Snow Removal and Ice Control Technology pp. 60-69. Washington, DC: Transportation Research Board, National Research Council. ISBN 0309062160.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • S.N. Freer
    • 1
  • B. Dien
    • 1
  • S. Matsuda
    • 2
  1. 1.National Center for Agricultural Utilization Research, USDA, Agricultural Research ServiceFermentation Biotechnology Research UnitPeoriaUSA
  2. 2.Kumamoto Industrial Research InstituteHigashimachiJapan

Personalised recommendations