Abstract
The main component of the ganglioside1 mixture from the brain of the adult amphibian Xenopus laevis accounts for 35% of the total, as lipid bound sialic acid. This ganglioside has been purified and characterized by thin layer chromatography, partial and exhaustive enzymatic hydrolysis with sialidase, TLC-overlay procedures with anti-Gg4Cer and anti-Neu5Acα6GalNAcβ specific monoclonal antibodies and mass spectrometry. All together the results suggest the following structure:
Neu5Acα8Neu5Acα3Galβ3(Neu5Acα8Neu5Acα6)GalNAcβ4Galβ4Glcβ1Cer
or, IV3-α-Neu5Ac2,III6-α-Neu5Ac2-Gg4Cer.
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Rizzo, A.M., Berra, B., Rossi, F. et al. Structure of the main ganglioside from the brain of Xenopus laevis . Glycoconj J 19, 53–57 (2002). https://doi.org/10.1023/A:1022584914778
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DOI: https://doi.org/10.1023/A:1022584914778