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Delivery, Distribution, and Neuronal Uptake of Exogenous Mannose-Terminal Glucocerebrosidase in the Intact Rat Brain

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Abstract

Gaucher disease is caused by insufficient activity of the enzyme glucocerebrosidase. Great benefit has been obtained through enzyme replacement therapy for patients with type 1 (non-neuronopathic) Gaucher disease. In contrast, inconsistent effects of enzyme therapy have been observed in patients with type 3 (chronic neuronopathic) Gaucher disease, and no benefit on the lethal course of the disease occurs in patients with Type 2 (acute neuronopathic) Gaucher disease. We examined the use of convection-enhanced delivery to augment the delivery and distribution of exogenous glucocerebrosidase (m.w. 63,000) to the brain by infusing it under slight hydrostatic pressure into the striatal region of rats. The enzyme was comparatively stable under these conditions. It was distributed from the site of injection toward the cerebral cortex where it became primarily localized in neurons. These findings provide considerable incentive for the exploration of intracerebral microinfusion of enzyme to the brain of patients with metabolic storage disorders involving the CNS.

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Author notes

  1. O. A. Sanchez

    Present address: Department of Neurology, University of Massachusetts Medical Center, Worchester, MA, 01655

Authors and Affiliations

  1. Developmental and Metabolic Neurology Branch, USA

    G. C. Zirzow, G. J. Murray & R. O. Brady

  2. National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892

    O. A. Sanchez & E. H. Oldfield

Authors
  1. G. C. Zirzow
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  2. O. A. Sanchez
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  3. G. J. Murray
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  4. R. O. Brady
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  5. E. H. Oldfield
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Zirzow, G.C., Sanchez, O.A., Murray, G.J. et al. Delivery, Distribution, and Neuronal Uptake of Exogenous Mannose-Terminal Glucocerebrosidase in the Intact Rat Brain. Neurochem Res 24, 301–305 (1999). https://doi.org/10.1023/A:1022578424693

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