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Systemic Induction of Terpenoid Aldehydes in Cotton Pigment Glands by Feeding of Larval Spodoptera exigua

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Abstract

Pigment glands in cotton contain terpenoid aldehydes that are toxic and deterrent to feeding of several generalist lepidopteran insects. We hypothesized that previously observed systemically induced feeding deterrence may be associated with pigment glands. We conducted experiments to determine the dynamics and chemical nature of inducible feeding deterrents in leaves of cotton, Gossypium hirsutum L, to larvae of the beet armyworm, Spodoptera exigua. Production and/or filling of pigment glands was influenced by physiological age of Deltapine 90 cotton plants. In undamaged plants, successively formed leaves contained more pigment glands, up to the seventh or eighth true-leaf developmental stage. Feeding choice tests conducted one or seven days after initial feeding damage revealed that third instars of S. exigua consumed more of the two youngest leaves from control cotton plants than from plants whose two oldest leaves had been fed on previously for 24 hr by S. exigua. The preference for leaves from control plants was significant one day after initial damage and highly significant seven days after damage. Consumption of mature foliage (leaf immediately above initially damaged leaves) from control plants and damaged plants did not differ. More pigment glands were counted on the youngest leaf of damaged plants than on the youngest leaf of control plants one day after initial damage. HPLC analysis revealed greater amounts of hemigossypolone, heliocides 1 and 2 (H1 and H2), and total terpenoid aldehydes per gland in young foliage of damaged plants than control plants one day after initial injury. By seven days after initial injury, greater quantities of hemigossypolone and all heliocides except H4 were detected in young foliage from damaged plants compared to control plants. Concentrations of H1 per gland in young leaves from damaged plants increased the most of all terpenoid aldehydes measured (3.4× the amount found in leaves from control plants). Mature leaves from damaged plants did not contain more terpenoid aldehydes than mature leaves from control plants. We suggest that systemically induced feeding deterrence to S. exigua in young leaves of glanded cotton was due to increased amounts of terpenoid aldehydes in pigment glands.

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REFERENCES

  • ALBORN, H. T., RÖSE, U. S. R., and MCAUSLANE, H. J. 1966. Systemic induction of feeding deterrents in cotton plants by feeding of Spodoptera spp. larvae. J. Chem. Ecol. 22:919–932.

    Google Scholar 

  • ALTMAN, D. W., STIPANOVIC, R. D., and BELL, A. A. 1990. Terpenoids in foliar pigment glands of A, D, and AD genome cottons: introgression potential for pest resistance. J. Hered. 81:447–454.

    Google Scholar 

  • BELL, A. A. 1984. Morphology, chemistry and genetics of Gossypium adaptations to pests. Recent Adv. Phytochem. 18:197–230.

    Google Scholar 

  • BELL, A. A., and STIPANOVIC, R. D. 1976. Characterization of terpenoids associated with glands of young cotton leaves, pp. 52–54, in Proceedings of the Beltwide Cotton Production Research Conferences. January 5–7, Las Vegas, Nevada. 28th Cotton Improvement Conference. National Cotton Council, Memphis, Tennessee.

    Google Scholar 

  • BELL, A. A., STIPANOVIC, R. D., ELZEN, G. W., and WILLIAMS, H. J., JR. 1987. Structural and genetic variation of natural pesticides in pigment glands of cotton (Gossypium) pp. 477–490, in G. R. Waller (ed.). Allelochemicals: Role in Agriculture and Forestry. ACS Symposium Series 330. American Chemical Society, Washington, DC.

    Google Scholar 

  • BOTTGER, G. T., and PATANA, R. 1966. Growth, development, and survival of certain Lepidoptera fed gossypol in the diet. J. Econ. Entomol. 59:1166–1168.

    Google Scholar 

  • BOTTGER, G. T., SHEEHAN, E. T., and LUKEFAHR, M. J. 1964. Relation of gossypol content of cotton plants to insect resistance. J. Econ. Entomol. 57:283–285.

    Google Scholar 

  • CROXFORD, A. C., EDWARDS, P. J., and WRATTEN, S. D. 1989. Temporal and spatial variation in palatability of soybean and cotton leaves following wounding. Oecologia 79:520–525.

    Google Scholar 

  • ESSENBERG, M., DOHERTY, M. d'A., HAMILTON, B. K., HENNING, V. T., COVER, E. C., MCFAUL, S. J., and JOHNSON, W. M. 1982. Identification and effects on Xanthomonas campestris pv. malvacearum of two phytoalexins from leaves and cotyledons of resistant cotton. Phytopathology 72:1349–1356.

    Google Scholar 

  • HEDIN, P. A., and MCCARTY, J. C., JR. 1991. Effects of kinetin formulations on allelochemicals and agronomic traits of cotton. J. Agric. Food Chem. 39:549–553.

    Google Scholar 

  • HEDIN, P. A., PARROTT, W. L., and JENKINS, J. N. 1991. Effects of cotton plant allelochemicals and nutrients on behavior and development of tobacco budworm. J. Chem. Ecol. 17:1107–1121.

    Google Scholar 

  • HEDIN, P. A., PARROTT, W. L., and JENKINS, J. N. 1992. Relationships of glands, cotton square terpenoid aldehydes, and other allelochemicals to larval growth of Heliothis virescens (Lepidoptera: Noctuidae). J. Econ. Entomol. 85:359–364.

    Google Scholar 

  • JOHNSON, S. J. 1984. Larval development, consumption, and feeding behavior of the cotton leafworm, Alabama argillacea (Hübner). Southwest. Entomol. 9:1–6.

    Google Scholar 

  • KARBAN, R., and CAREY, J. R. 1984. Induced resistance of cotton seedlings to mites. Science 225:53–54.

    Google Scholar 

  • KHOSHKHOO, N., HEDIN, P. A., and MCCARTY, J. C., JR. 1993. Effects of bioregulators on the terpenoid aldehydes in root-knot nematode infected cotton plants. J. Agric. Food Chem. 41:2442–2446.

    Google Scholar 

  • KHOSHKHOO, N., HEDIN, P. A., and MCCARTY, J. C., JR. 1994. Terpenoid aldehydes in root-knot nematode susceptible and resistant cotton plants. J. Agric. Food Chem. 42:204–208.

    Google Scholar 

  • KING, E. G., and LEPPLA, N. C. 1984. Advances and Challenges in Insect Rearing. Agriculture Research Service, USDA. US Government Printing Office, Washington, DC.

    Google Scholar 

  • LOUGHRIN, J. H., MANUKIAN, A., HEATH, R. R., TURLINGS, T. C. J., and TUMLINSON, J. H. 1994. Diurnal cycle of emission of induced volatile terpenoids by herbivore-injured cotton plants. Proc. Natl. Acad. Sci. U.S.A. 91:11836–11840.

    Google Scholar 

  • LUKEFAHR, M. J., and FRYXELL, P. A. 1967. Content of gossypol in plants belonging to genera related to cotton. Econ. Bot. 21:128–131.

    Google Scholar 

  • LUKEFAHR, M. J., STIPANOVIC, R. D., BELL, A. A., and GRAY, J. R. 1977. Biological activity of new terpenoid compounds from Gossypium hirsutum against the tobacco budworm and pink bollworm, pp. 97–100, in Proceedings of the Beltwide Cotton Production Research Conferences. January 10–12, Atlanta, Georgia. 29th Cotton Improvement Conference. National Cotton Council, Memphis, Tennessee.

    Google Scholar 

  • MONTANDON, R., WILLIAMS, H. J., STERLING, W. L., STIPANOVIC, R. D., and VINSON, S. B. 1986. Comparison of the development of Alabama argillacea (Hübner) and Heliothis virescens (F.) (Lepidoptera: Noctuidae) fed glanded and glandless cotton leaves. Environ. Entomol. 15:128–131.

    Google Scholar 

  • PARÉ, P. W., and TUMLINSON, J. H. 1997. Induced synthesis of plant volatiles. Nature 385:30–31.

    Google Scholar 

  • PARROTT, W. L., JENKINS, J. N., and MCCARTY, J. C. JR. 1983. Feeding behavior of first-stage tobacco budworm (Lepidoptera: Noctuidae) on three cotton cultivars. Ann. Entomol. Soc. Am. 76:167–170.

    Google Scholar 

  • PARROTT, W. L., JENKINS, J. N., MULROONEY, J. E., MCCARTY, J. C. JR., and SHEPHERD, R. L. 1989. Relationship between gossypol gland density on cotton squares and resistance to tobacco budworm (Lepidoptera: Noctuidae) larvae. J. Econ. Entomol. 82:589–592.

    Google Scholar 

  • RÖSE, U. S. R., MANUKIAN, A., HEATH, R. R., and TUMLINSON, J. H. 1996. Volatile semiochemicals released from undamaged cotton leaves: A systemic response of living plants to caterpillar damage. Plant Physiol. 111:487–495.

    Google Scholar 

  • SAS INSTITUTE. 1995. Guide for personal computers, version 6. SAS Institute, Cary, North Carolina.

    Google Scholar 

  • SHAVER, T. N., and LUKEFAHR, M. J. 1969. Effect of flavonoid pigments and gossypol on growth and development of the bollworm, tobacco budworm, and pink bollworm. J. Econ. Entomol. 62:643–646.

    Google Scholar 

  • SHAVER, T. N., and PARROTT, W. L. 1970. Relationship of larval age to toxicity of gossypol to bollworms, tobacco budworms, and pink bollworms. J. Econ. Entomol. 63:1802–1804.

    Google Scholar 

  • STIPANOVIC, R. D., BELL, A. A., and LUKEFAHR, M. J. 1977a. Natural insecticides from cotton (Gossypium), pp. 197–214, in P. A. Hedin (ed.). Host Plant Resistance to Insects. ACS Symposium Series 62. American Chemical Society, Washington, DC.

    Google Scholar 

  • STIPANOVIC, R. D., BELL, A. A., O'BRIEN, D. H., and LUKEFAHR, M. J. 1977b. Heliocide H2: an insecticidal sesterterpenoid from cotton (Gossypium). Tetrahedron Lett. 6:567–570.

    Google Scholar 

  • STIPANOVIC, R. D., BELL, A. A., O'BRIEN, D. H., and LUKEFAHR, M. J. 1978a. Heliocide H1. A new insecticidal C25 terpenoid from cotton (Gossypium hirsutum). J. Agric. Food Chem. 26:115–118.

    Google Scholar 

  • STIPANOVIC, R. D., BELL, A. A., O'BRIEN, D. H., and LUKEFAHR, M. J. 1978b. Heliocide H3 an insecticidal terpenoid from Gossypium hirsutum. Phytochemistry 17:151–152.

    Google Scholar 

  • STIPANOVIC, R. D., ALTMAN, D. W., BEGIN, D. L., GREENBLATT, G. A., and BENEDICT, J. H. 1988. Terpenoid aldehydes in upland cottons: analysis by aniline and HPLC methods. J. Agric. Food Chem. 36:509–515.

    Google Scholar 

  • STIPANOVIC, R. D., MACE, M. E., ELISSALDE, M. H., and BELL, A. A. 1991. Desoxyhemigossypol, a cotton phytoalexin: Structure-activity relationship. pp. 336–351, in P. A. Hedin (ed.), Naturally Occurring Pest Bioregulators. ACS Symposium Series 449. American Chemical Society, Washington, DC.

    Google Scholar 

  • VEECH, J. A., STIPANOVIC, R. D., and BELL, A. A. 1976. Peroxidative conversion of hemigossypol to gossypol. A revised structure for isohemigossypol. J. Chem. Soc., Chem. Commun. 4:144–145.

    Google Scholar 

  • WILLIAMS, H. J., STIPANOVIC, R. D., SMITH, L. A., VINSON, S. B., DARNELL, P. O., MONTANDON, R., BEGIN, D. L., ELZEN, G. W., GUANASENA, H., and BELL, A. A. 1987. Effects of gossypol and other terpenoids on Heliothis virescens development. Rev. Latinoam. Quim. 18:119–131.

    Google Scholar 

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Authors and Affiliations

  1. Department of Entomology & Nematology, University of Florida, PO Box 110620, Gainesville, Florida, 32611-0620

    Heather J. McAuslane

  2. USDA-ARS-SEA CMAVE, PO Box 14565, Gainesville, Florida, 32604-2565

    Hans T. Alborn

  3. Food Sciences and Human Nutrition, University of Florida, PO Box 110720, Gainesville, Florida, 32611-0720

    John P. Toth

Authors
  1. Heather J. McAuslane
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  2. Hans T. Alborn
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  3. John P. Toth
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McAuslane, H.J., Alborn, H.T. & Toth, J.P. Systemic Induction of Terpenoid Aldehydes in Cotton Pigment Glands by Feeding of Larval Spodoptera exigua . J Chem Ecol 23, 2861–2879 (1997). https://doi.org/10.1023/A:1022575313325

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