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Fluorescence In Situ Hybridization with Chromosome Paint Probes: A Novel Approach To Assess Aneuploidy in Human Sperm Nuclei

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Abstract

Purpose: Fluorescence in situ hybridization (FISH) using whole-chromosome paint probes was performed to evaluate disomy and diploidy frequency for chromosomes 1, 18, 19, and 22 in human sperm nuclei.

Methods: Ten subjects of proven fertility and normal spermatic parameters were included in the study. A dual-color FISH method was carried out.

Results: A total of 157,896 spermatozoa was scored. The mean frequencies of disomic sperm for chromosomes 1, 18, 19, and 22 were 0.22% (range, 0.19 to 0.28%), 0.24% (range, 0.14 to 0.37%), 0.22% (range, 0.17 to 0.30%), and 0.25% (range, 0.21 to 0.29%), respectively. The mean frequency of diploidy was 0.14% (range, 0.09 to 0.18%). No interindividual and interchromosomal variations in the aneuploidy frequency were observed between the different subjects.

Conclusions: FISH with whole-chromosome paint probes provides a novel and efficient approach for disomy assessment in human sperm nuclei.

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Authors and Affiliations

  1. Reproductive Biology Laboratory, Rouen University Hospital, Rouen Cedex, France

    N. Rives, S. Wust, B. David, V. Duchesne, G. Joly & B. Mace

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  1. N. Rives
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  2. S. Wust
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  3. B. David
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  4. V. Duchesne
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  5. G. Joly
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  6. B. Mace
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Rives, N., Wust, S., David, B. et al. Fluorescence In Situ Hybridization with Chromosome Paint Probes: A Novel Approach To Assess Aneuploidy in Human Sperm Nuclei. J Assist Reprod Genet 16, 46–51 (1999). https://doi.org/10.1023/A:1022549713692

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