Abstract
Lipase production by the potato pathogen Fusarium oxysporum AM3 was investigated in a mineral medium using triolein and sodium nitrate as carbon and nitrogen sources, respectively. Medium design by factorial analysis of the medium components increased enzyme activity 9.4-folds over the standard medium. The simple medium composition promoted easy enzyme recovery to its homogeneity in a single step. The lipase showed optimum activity at pH 9.0 and 35 °C, with a K M value of 7.5 mM for triolein and apparent molecular weight of 29.0 kDa. When assayed with different solvents, FoxAM3 lipase showed an increase on its activity by isooctane, isopropanol and acetone.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergmeyer, H.U. 1985 Methods in enzymatic analysis, 3rd ed. vols. IV and V. Weinheim: Verlag-Chemie. ISBN 3-527-26048-X.
Bradford, M.M. 1976 Arapid and sensitive method for quantitative of microgram quantities of protein utilising the principle of protein-dye binding. Analitical Biochemistry 72, 248-254.
Camargo-de-Morais, M.M., Morais, M.A. Jr., Melo, E.H.M. & Lima-Filho, J.L. 1998 Production of extracellular lipase by a Candida rugosa strain isolated in Pernambuco, Brazil. Brazilian Journal of Microbiology 29, 134-137.
Chowdary, G.V., Ramesh, M.N. & Prapulla, S.G. 2000 Enzymatic synthesis of isoamyl isovalerate using immobilized lipase from Rhizomucor miehei: a multivariate analysis. Process Biochemistry 36, 331-339.
Cruz, P.M., Christen, P. & Farres, A. 1993 Medium optimization by fractional factorial design for lipase production by Rhizipus delemar. Journal of Fermentation and Bioengineering 76, 94-97.
Garcia-Lepe, R., Nuero, O.M., Reyes, F. & Santamaria, F. 1997 Lipases in autolysed cultures of filamentous fungi. Letters in Applied Microbiology 25, 127-130.
Hatzinikolaou, D.G., Macris, J.B., Christakopoulos, P., Kekos, D., Kolisis, F.N. & Fountoukidis, G. 1996 Production and partial characterization of extracellular lipase from Aspergillus niger. Biotechnology Letters 18, 547-552.
Jaeger, K.E. & Reetz, M.T. 1998 Microbial lipases form versatile tools for biotechnology. Trends in Biochemistry 16, 396-403.
Maia, M.M.D., Heasley, A., Camargo-de-Morais, M.M., Melo, E.H., Morais, M.A. Jr., Ledingham, W.M. & Lima Filho, J.L. 2001 Effect of culture conditions on lipase production by Fusarium solani in batch fermentation. Bioresource Technology 76, 23-27.
Minning, S., Serrano, A., Ferrer, P., Sola, C., Schmid, R.D. & Valero, F. 2001 Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. Journal of Biotechnology 86, 59-70.
Overbeeke, P.L.A., Jongejan, J.A. & Heijnen, J.J. 2000 Solvent effect on lipase enatioselectivity. Evidence for the presence of two thermodynamic states. Biotechnology and Bioengineering 70, 278-290.
Pimentel, M.C.B., Krieger, N., Coelho, L.C.C.B., Fontana, J.O., Melo, E.H.M., Ledingham, W.M. & Lima-Filho, J.L. 1994 Lipase from a Brazilian strain of Penicillium citrinum. Applied Biochemistry and Biotechnology 49, 59-74.
Rapp, P. 1995 Production, regulation and some properties of lipase activity from Fusarium oxysporum f. sp. vasinfectum. Enzyme and Microbial Technology 17, 832-838.
Scopes, R.K. 1994 Protein Purification: Principles and Practice, 3rd ed. pp. 76-85. New York: Springer-Verlag. ISBN 3-540-94072-3.
Shimada, S. & Tominaga, Y. 1993 Purification and characterization of a novel solvent-tolerant lipase from Fusarium heterosporum. Journal of Fermentation and Bioengeneering 75, 349-352.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Camargo-de-Morais, M., Maia, M., Borba, F. et al. Oil/mineral-salts medium designed for easy recovery of extracellular lipase from Fusarium oxysporum AM3. World Journal of Microbiology and Biotechnology 19, 17–20 (2003). https://doi.org/10.1023/A:1022543125420
Issue Date:
DOI: https://doi.org/10.1023/A:1022543125420