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Characterization of T cell hybridomas raised against a glycopeptide containing the tumor-associated T antigen, (βGal (1–3) αGalNAc-O/Ser)

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Abstract

T cell hybridomas were raised against the glycopeptide S72 (Core-1) containing the tumor-associated disaccharide βGal (1–3) αGalNAc (Core-1) O-linked to serine at position 72 in the mouse hemoglobin derived decapeptide Hb (67–76). All hybridomas recognized the glycopeptide S72 (Core-1). Two of the selected hybridomas responded, however, much better to the S72 (Tn) glycopeptide containing the monosaccharide αGalNAc O-linked to serine. In addition, one hybridoma cross-responded to the glycopeptide T72 (Core-1) having a threonine at position 72 instead of a serine. No cross-responses were found to other glycopeptides consisting of the same hemoglobin peptide with different glycans attached or to the unglycosylated peptides. The T cell receptor Vα and Vβ usage was clearly diverse. The CDR3α regions demonstrated moreover a predominance of small polar amino acid side chains, and three hybridomas contained a common sequence motif. All the sequenced CDR3β regions contained furthermore a conserved proline-glycine motif. In conclusion, immunization with the disaccharide containing glycopeptides S72 (Core-1) created a heterogeneous population of glycopeptide specific T cells with the ability of cross-responding toward related glycopeptides.

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Authors and Affiliations

  1. Institute for Medical Microbiology and Immunology, University of Copenhagen, Denmark

    Monika Gad, Ole Werdelin & Teis Jensen

  2. The Carlsberg Laboratory, Department of Chemistry, Copenhagen, Denmark

    Morten Meldal & Shiro Komba

  3. Pharmexa A/S, Hørsholm, Denmark

    Teis Jensen

Authors
  1. Monika Gad
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  2. Ole Werdelin
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  3. Morten Meldal
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  4. Shiro Komba
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  5. Teis Jensen
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Correspondence to Teis Jensen.

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Gad, M., Werdelin, O., Meldal, M. et al. Characterization of T cell hybridomas raised against a glycopeptide containing the tumor-associated T antigen, (βGal (1–3) αGalNAc-O/Ser). Glycoconj J 19, 59–65 (2002). https://doi.org/10.1023/A:1022537031617

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