Abstract
Information concerning the sugar status of plant cells is of greatimportance during all stages of the plant life cycle. The aim of this work wasto study primary carbohydrate metabolism in hairy roots of red beet. Growth ofhairy roots of red beet in vitro and changes in concentration of major nutrientsand sugar in the media were measured over a growth cycle of 16 days. We havealso determined the levels of key enzymes in the pathways of sucrose metabolism.Sucrose concentration decreased as hairy root growth proceeded while no changein glucose and fructose levels in the medium was found during the first 3 daysindicating that external sucrose is preferably taken to the cell before it ishydrolyzed by extracellular invertase. The increase in glucose and fructoselevels in the media after 5 days of culture indicates extracellular hydrolysisof sucrose which was further supported by the activity of acid invertaseobserved during that time in the culture medium. The uptake of mineral nutrientsby hairy root of red beet was monitored continuously during the culture cycle.The preferential use of NH4 + overNO3 − at the beginning of the culture andacidification of culture media were the two most notable results concerningnitrogen nutrition during hairy root growth of red beet.
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Ashihara H., Horikosi T., Li X.N., Sagishima K. and Yamashita Y. 1988. Profiles of enzymes involved in glycolysis in Catharanthus roseuscells in batch suspension culture. J. Plant Physiol. 133: 38-45.
Chaplin M.F. 1986. Monosaccharides. In: Chaplin M.F. and Kennedy J.F. (eds), Carbohydrate Analysis. A Practical Approach. IRL Press Ltd., Oxford, pp. 1-36.
Cheng W.H., Taliercio E.W. and Chourey P.S. 1999. Sugars modulate an unusual mode of control of the cell-wall invertase gene (Incw1) through its 3′ untranslated region in a cell suspension culture of maize. Proc. Natl. Acad. Sci. USA 96: 10512-10517.
Chin C.K., Haas J.C. and Still C.C. 1981. Growth and sugar uptake of excised root and callus of tomato. Plant Sci. Lett 21: 229-234.
Corchete M.P., Jimenez M.A., Moran M., Cacho M. and Fernandez-Tarrago J. 1991. Effect of calcium, manganese and lithium on growth and cardenolide content in cell suspension cultures of Digitalis thapsiL. Plant Cell Rep. 10: 394-396.
Cuadrado Y., Guerra H., Martin A.B., Gallego P., Hita O., Dorado A. et al. 2001. Differences in invertase activity in embryogenic and non-embryogenic calli from Medicago arborea. Plant Cell Tiss. Org. Cult. 67: 145-151.
Daie J., Chin C. and Pitcher L. 1987. Differential rates of sucrose and hexose transport by asparagus cell cultures. Plant Sci. 53: 101-107.
Dantas A.K., Majada J.P., Fernandez B. and Canal M.J. 2001. Mineral nutrition in carnation tissue cultures under different ventilation conditions. Plant Growth Regul. 33: 237-243.
De Eknamkul W. and Ellis B.E. 1985. Effects of macronutrients on growth and rosmarinic acid formation in cell suspension cultures of Anchusa officinalis. Plant Cell Rep. 4: 46-49.
Dussert S., Verdeil J.L., Rival A., Noirot M. and Medel J.B. 1995. Nutrient uptake and growth of in vitro coconut (Cocos nuciferaL.) calluses. Plant Sci. 106: 185-193.
El Badaoui H., Morard P. and Henry M. 1996. Stimulation of the growth and solamargine production by Solanum paludosummultiple shoot cultures using a new culture medium. Plant Cell Tiss. Org. Cult. 45: 153-158.
Kanabus J., Bressan A.R.A. and Carpita N.C. 1986. Carbon assimilation in carrot cells in liquid culture. Plant Physiol. 82: 363–368.
Kaur N., Jain H., Mann P., Gupta A.K. and Singh R.A. 1992. Comparison of properties of invertases and inulinase from chicory. Plant Physiol. Biochem. 304: 445-450.
Khuri S. and Moorby J. 1995. Investigation into the role of sucrose in potato cv. Estima microtuber production in vitro. Ann. Bot. 75: 295-303.
Lemoine R., Daie J. and Wyse R. 1988. Evidence for the presence for the presence of a sucrose carrier in immature sugar beet tap roots. Plant Physiol. 86: 575-580.
Lemoine R. 2000. Sucrose transporters in plants: update on function and structure. Biochim. Biophys. Acta. 1467: 246-262.
Lowry D.H., Rosebrough N.J., Farr A.L. and Randall R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
Masuda H., Toshimasa T. and Sugawara S. 1988. Acid and alkaline invertases in suspension cultures of sugar beet cells. Plant Physiol 86: 312-317.
Morard P., Fulcheri C. and Henry M. 1998. Kinetics of mineral nutrient uptake by Saponaria officinalisL. suspension cell cultures in different media. Plant Cell Rep. 18: 260-265.
Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bio-assay with tobacco tissue cultures. Physiol. Plant 15: 327-332.
Nour K.A. and Thorpe T.A. 1994. The effect of the gaseous state on bud induction and shoot multiplication in vitro in eastern white cedar. Physiol. Plant 90: 163-172.
Roitsch T., Bittner M. and Godt D.E. 1995. Induction of apoplastic invertase of Chenopodium rubrumby D-glucose and a glucose analog and tissue-specific expression suggest a role in sinksource regulation. Plant Physiol. 108: 285-294.
Sallanon H., Isaka H., Dimon B., Ravel C. and Chagvardieff P. 1997. CO2 exchanges and nutrient uptake during multiplication and rooting of micropropagated Juglans regiaplantlets. Plant Sci. 124: 107-116.
Smeekens S. and Rook F. 1997. Sugar sensing and sugar-mediated signal transduction in plants. Plant Physiol. 115: 7-13.
Taya M., Mine K., Kino-Oka M., Tone S. and Ichi T. 1992. Production and release of pigments by culture of transfomed hairy root of red beet. J. Ferment. Bioengng. 73: 31-36.
Van den Ende W. and Van Laere A. 1995. Purification and properties of a neutral invertase from the roots of Cichorium intybus. Physiol. Plant 593: 241-248.
Van Huylenbroeck J.M. and De Riek J. 1995. Sugar and starch metabolism during ex vitro rooting and acclimatization of micropropagated Spathiphyllum'Petite' plantlets. Plant Sci. 111: 19-25.
Venkataramana S., Naidu K.M. and Singh S. 1991. Invertases and growth factors dependent sucrose accumulation in sugarcane. Plant Sci. 74: 65-72.
Wyse R. 1979. Sucrose uptake by sugar beet tap root tissue. Plant Physiol. 64: 837-841.
Singh B.D., Singh V.K., Singh R.B. and Singh R.M. 1978. Effects of buffers on carrot callus and suspension cultures. Indian J. Exp. Biol. 16: 842-843.
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Shin, KS., Chakrabarty, D., Ko, JY. et al. Sucrose utilization and mineral nutrient uptake during hairy root growth of red beet (Beta vulgaris L.) in liquid culture. Plant Growth Regulation 39, 187–193 (2003). https://doi.org/10.1023/A:1022525308389
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DOI: https://doi.org/10.1023/A:1022525308389