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Myelin Basic Protein and Myelin Basic Protein Peptides Induce the Proliferation of Schwann Cells Via Ganglioside GM1 and the FGF Receptor

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Abstract

Myelin basic protein (MBP) and two peptides derived from MBP (MBP1–44 and MBP152–167) stimulated Schwann cell (SC) proliferation in a cAMP-mediated process. The two mitogenic regions of MBP did not compete with one another for binding to SC suggesting a distinctive SC receptor for each mitogenic peptide. Neutralizing antibodies to the fibroblast growth factor receptor blocked the mitogenic effect of the myelin-related SC mitogen found in the supernatant of myelin-fed macrophages. The binding of 125I-MBP to Schwann cells was specifically inhibited by basic fibroblast growth factor (bFGF) and conversely the binding of 125I-bFGF was competitively inhibited by MBP. These data suggested that the mitogenic effect of one MBP peptide was mediated by a bFGF receptor. The binding of MBP to ganglioside GM1 and the ability of MBP peptides containing homology to the B subunit of cholera toxin (which binds ganglioside GM1) to compete for the binding of a mitogenic peptide (MBP1–44) to SC, identified ganglioside GM1 as a second SC receptor. Based on these results, we conclude that MBP1–44 and MBP152–167 associate with ganglioside GM1 and the bFGF receptor respectively to stimulate SC mitosis.

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Author notes

  1. Shun-Fen Tzeng

    Present address: Department of Medical Research and Education, Taichung VA Hospital, Taichung, Taiwan, Republic of China

Authors and Affiliations

  1. Research Service, Hines/VA Hospital, Hines, Illinois, 60141

    George H. DeVries

  2. Laboratory of Cerebral Metabolism, NIMH-NIH, Bethesda, MD, 20892

    Gladys E. Deibler

  3. Department of Cell Biology, Neurobiology and Anatomy, Stritch School of Medicine-Loyola University Chicago, Maywood, Illinois, 60153

    George H. DeVries

Authors
  1. Shun-Fen Tzeng
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  2. Gladys E. Deibler
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  3. George H. DeVries
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Tzeng, SF., Deibler, G.E. & DeVries, G.H. Myelin Basic Protein and Myelin Basic Protein Peptides Induce the Proliferation of Schwann Cells Via Ganglioside GM1 and the FGF Receptor. Neurochem Res 24, 255–260 (1999). https://doi.org/10.1023/A:1022514105129

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