Abstract
1. The present study reexamines a previous notion on opioid stimulation of cyclic GMP (cGMP) formation and the retraction of the original findings.
2. The effect of opioid agonists on cGMP accumulation in two cell lines of neuronal origin was measured. The proportion of cGMP stimulation in NG108-15 neuroblastoma × glioma hybrid cells resembled the proportion of [Ca2+]in elevation by opioids in this culture. The failure of opioids to stimulate cGMP formation in SK-N-SH human neuroblastoma coincided with the lack of cGMP stimulation by other Ca2+ mobilizing agents in these cells. The nitric oxide donor nitroprusside elevated cGMP in both cell lines.
3. The implication of the opioid-Ca2+-NO-cGMP cellular pathway for opioid activity in vivo is discussed.
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REFERENCES
Bhargava, H. N. (1995). Attenuation of tolerance to, and physical dependence on, morphine in the rat by inhibition of nitric oxide synthase. Gen. Pharmacol. 26:1049–1059.
Bredt, D. S., and Snyder, S. H. (1992). Nitric oxide, a novel neuronal messenger. Neuron 8:3–11.
Burton, C. K., Ho, I. K., and Hoskins, B. (1990). Evidence for involvement of cyclic GMP phosphodiesterase in morphine tolerance. J. Pharmacol. Exp. Ther. 252:104–111.
Cappendijk, S. L. T., de Vries, R., and Dzoljic, M. R. (1993). Inhibitory effect of nitric oxide (NO) synthase inhibitors on naloxone-precipitated withdrawal syndrome in morphine-dependent mice. Neurosci. Lett. 162:97–100.
Conor, M. A., Planner, A., and Henderson, G. (1994). δ and μ opioid receptor mobilization of intracellular calcium in neuroblastoma cells. Regul. Peptides 54:65–66.
Dambisya, Y. M., and Lee, T. L. (1996). Role of nitric oxide in the induction and expression of morphine tolerance and dependence in mice. Br. J. Pharmacol. 117:914–918.
Ferreira, S. H., Duarte, I. D. G., and Lorenzetti, B. B. (1991). The molecular mechanism of action of peripheral morphine analgesia: Stimulation of the cGMP system via nitric oxide release. Eur. J. Pharmacol. 201:121–122.
Fields, A., Gafni, M., Oron, Y., and Sarne, Y. (1995). Multiple effects of opiates on intracellular calcium level and on calcium uptake in three neuronal cell lines. Brain Res. 687:94–102.
Goy, M. F. (1991). cGMP: The wayward child of cyclic nucleotide family. TINS 14:293–299.
Gullis, R., Traber, J., and Hamprecht, B. (1975). Morphine elevates levels of cyclic GMP in a neuroblastoma × glioma hybrid cell line. Nature 256:57–59.
Gwynn, G. J., and Costa, E. (1982). Opioids regulate cGMP formation in cloned neuroblastoma cells. Proc. Natl. Acad. Sci. USA 79:690–694.
Hamprecht, B., and Gullis, R. J. (1997). Statement, Nature 265:764.
Jin, W., Lee, N. M., Loh, H. H., and Thayer, S. A. (1992). Dual excitatory and inhibitory effects of opioids on intracellular calcium in neuroblastoma × glioma NG108-15 cells. Mol. Pharmacol. 42:1083–1089.
Jin, W., Lee, N. M., Loh, H. H., and Thayer, S. A. (1994). Opioids mobilize calcium from inositol 1,4,5-triphosphate-sensitive stores in NG108-15 cells. J. Neurosci. 14:1920–1929.
Kolesnikov, Y. A., Pick, C. G., and Pasternak, G. W. (1992). NG-Nitro-L-arginine prevents morphine tolerance. Eur. J. Pharmacol. 221:399–400.
Minneman, K. P., and Iversen, L. L. (1976). Enkephalin and opiate narcotics increase cyclic GMP accumulation in slices of rat neostratium. Nature 262:313–314.
Okajima, F., and Kondo, Y. (1992). Synergism in cytosolic Ca2+ mobilization between bradykinin and agonists for pertussis toxin-sensitive G-protein-coupled receptors in NG108-15 cells. FEBS Lett. 301:223–226.
Okajima, F., Tomura, H., and Kondo, Y. (1993). Enkephalin activates the phospholipase C/Ca2+ system through cross-talk between opioid receptors and P2 purinergic or bradykinin receptors in NG108-15 cells. Biochem. J. 290:241–247.
Racagni, G., Zsilla, G., Guidotti, A., and Costa, E. (1976). Accumulation of cGMP in striatum of rats injected with narcotic analgesics: Antagonism by maltrexone. J. Pharm. Pharmacol. 28:258–260.
Reiser, G. (1990). Mechanism of stimulation of cyclic-GMP level in a neuronal cell line mediated by serotonin (5-HT3) receptors. Eur. J. Biochem. 189:547–552.
Reiser, G. (1995). Ca2+ and nitric oxide-dependent stimulation of cyclic GMP synthesis in neuronal cell line induced by P2-purinergic/pyrimidinergic receptor. J. Neurochem. 64:61–68.
Reiser, G., Walter, U., and Hamprecht, B. (1984). Bradykinin regulates the level of guanosine 3′,5′-cyclic monophosphate (cyclic GMP) in neuronal cell lines, Brain Res. 290:367–371.
Sarne, Y., and Gafni, M. (1996). Determinants of the stimulatory opioid effect on intracellular calcium in SK-N-SH and NG108-15 neuroblastoma. Brain Res. 722:203–206.
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Sarne, Y., Gafni, M. & Fields, A. Reexamination of Opioid Stimulation of cGMP Formation in Cell Lines of Neuronal Origin. Cell Mol Neurobiol 18, 425–428 (1998). https://doi.org/10.1023/A:1022501632502
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DOI: https://doi.org/10.1023/A:1022501632502