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Identifying Monoaminergic, GABAergic, and Cholinergic Characteristics in Immortalized Neuronal Cell Lines

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Abstract

We measured the concentration of neurotransmitters in immortalized neural cell lines of hippo-campal, septal, brainstem and cerebellar origin. While in most of the cell lines, concentrations of monoamines, γ-aminobutyric acid (GABA) and acetylcholine were low, in some they were markedly higher. This made it quite easy to identify possible monoaminergic, GABAergic or cholinergic cell lines. However all the cell lines contained glutamate and aspartate and there were no outstanding differences in levels of these amino acids differences between the cell lines. Deprivation of serum, which made the cells acquire a more differentiated morphology, caused an increase in the intracellular concentrations of some compounds and a switch from multiple to a single transmitter in the case of some cell lines. It suggested that measurement of transmitter concentrations combined with serum deprivation studies, may provide an indication of the neurochemical characteristics of immortalised neuronal cell lines.

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Authors and Affiliations

  1. Division of Demyelinating Diseases and Ageing, National Institute of Neuroscience, NCNP, Tokyo, Japan

    Ferenc Gallyas Jr., Tatsuhide Kunishita & Takeshi Tabira

  2. Department of Internal Medicine, Saga Medical University, Saga, Japan

    Jun-ichi Satoh

  3. Department of Applied Immunology, Faculty of Agriculture, University of Tokyo, Tokyo, Japan

    Alice Mayumi Takeuchi

  4. Department of Neuropathology, Kawasaki Medical University, Kurashiki, Japan

    Yoshihiro Konishi

Authors
  1. Ferenc Gallyas Jr.
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  2. Jun-ichi Satoh
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  3. Alice Mayumi Takeuchi
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  4. Yoshihiro Konishi
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  5. Tatsuhide Kunishita
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  6. Takeshi Tabira
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Gallyas, F., Satoh, Ji., Takeuchi, A.M. et al. Identifying Monoaminergic, GABAergic, and Cholinergic Characteristics in Immortalized Neuronal Cell Lines. Neurochem Res 22, 569–575 (1997). https://doi.org/10.1023/A:1022465918695

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