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Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques

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Abstract

Direct EPR evidence of the photo-generation of superoxide radicals (O2 −.) was obtained by using a novel spin trapping probe in spinach Photosystem II (PS II) membrane fragments. The production of O2 −. was detected by following the formation of 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) superoxide adducts (DEPMPO-OOH). The inhibition of O2 −. formation by 3-(3,4-dichlorophenyl) -1,1-dimethylurea (DCMU) and the 77 K fluorescence spectrum indicated that O2 −. were generated from PS II, not from PS I. The inhibition of O2 −. formation by DCMU also suggested that O2 −. were generated from the QBbinding site, not at a site prior to DCMU blockage. The extrinsic proteins and Mn are very important to eliminate O2 −., showing that the oxygen-evolving system is involved in O2 −. removal rather than production.

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Authors and Affiliations

  1. Institute of Plant Physiology, Shanghai Institutes for the Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China

    Suping Zhang, Jun Weng, Jingxi Pan, Tiecheng Tu & Side Yao

  2. Institute of Plant Physiology, Shanghai Institutes for the Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China

    Chunhe Xu

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  1. Suping Zhang
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  2. Jun Weng
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  3. Jingxi Pan
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  4. Tiecheng Tu
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  5. Side Yao
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  6. Chunhe Xu
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Correspondence to Chunhe Xu.

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Zhang, S., Weng, J., Pan, J. et al. Study on the photo-generation of superoxide radicals in Photosystem II with EPR spin trapping techniques. Photosynthesis Research 75, 41–48 (2003). https://doi.org/10.1023/A:1022439009587

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