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Establishment of the mimetic aging effect in mice caused by D-galactose

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Abstract

We successfully established an oxidant damageof mimetic aging model using mice induced byD-galactose, and the mimetic aging model isrelative to free radical and the accumulationof waste substances in metabolism. The animalswere divided into 3 groups: (1)phosphate-buffered saline (PBS); (2) 1%D-galactose; (3) 5% D-galactose bysubcutaneous injection every day. After 45days, mice treated with D-galactose showed asignificant increase in the malondialdehyde(MDA), total antioxidant status (TAS) and adecrease in superoxide dismutase (SOD) in the blood comparedwith the PBS group. In the brain, theD-galactose treated mice exhibited a higherlevel MDA and a lower level SOD activity.In the liver, only the 5% D-galactose groupindicated a significant increase in MDA. By reference to theoxidative biomarkers in blood, brain and liver,we have confirmed the reliability of themimetic aging model.

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Authors and Affiliations

  1. Traditional Chinese Medicine Division, Development Center for Biotechnology, 81 Chang Hsing Street, Taipei, Taiwan, R.O.C.

    Shih-Ching Ho, Jue-Hao Liu & ReY-Yih Wu

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  1. Shih-Ching Ho
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  2. Jue-Hao Liu
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  3. ReY-Yih Wu
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Correspondence to Shih-Ching Ho.

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Ho, SC., Liu, JH. & Wu, RY. Establishment of the mimetic aging effect in mice caused by D-galactose. Biogerontology 4, 15–18 (2003). https://doi.org/10.1023/A:1022417102206

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