Skip to main content
SpringerLink
Log in

Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots

Plant Cell, Tissue and Organ Culture volume 72, pages 261–265 (2003)Cite this article

Abstract

This study was made to clarify the effects of cytokinin type and their concentrations on shoot proliferation and hyperhydricity in in vitro Pyrus pyrifolia N. (`Hosui' and `Kosui') shoots. The shoots were subcultured in a woody plant medium supplemented with 0.5 μM 3-indolyl-butyric acid, 3% (w/v) sorbitol, 0.8% (w/v) agar, and with cytokinins kinetin, 6-benzylaminopurine (BA), N-(2-chloro-4-pyridyl)-N9-phenylurea (CPPU), 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (TDZ) added at concentrations 0.44, 4.40, 11.0 and 44.0 μM. The highest number of shoots was induced by adding BA at concentration 11.0 μM, then by 4.4 μM BA, in both cultivars. TDZ and CPPU caused greater hyperhydricity in cultured explants than BA and kinetin. `Kosui' was more susceptible to hyperhydricity compared with `Hosui'.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  • Andrade LB, Echeverrigaray S, Fracaro F, Pauletti GF & Rota L (1999) The effect of growth regulators on shoot propagation and rooting of common lavendar (Lavandura vera DC). Plant Cell Tiss. Org. Cult. 56: 79-83

    Google Scholar 

  • Banno K, Yoshida K, Hayashi S & Tanabe K (1989) In vitro propagation of Japanese pear cultivars. J. Jpn. Soc. Hort. Sci. 58: 37-42

    Google Scholar 

  • Briggs BA, McCulloch SM & Edick LA (1988a) Micropropagation of azaleas using thidazuron. Acta Hort. 226: 205-208

    Google Scholar 

  • Briggs BA, McCulloch SM & Edick LA (1988b) Micropropagation of azaleas using thidazuron. Acta Hort. 227: 330-333

    Google Scholar 

  • Gray DJ & Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia) Plant Cell Tiss. Org. Cult. 27: 7-14

    Google Scholar 

  • Gribaudo I & Fronda A (1991) Effects of thidiazuron on grapevine axillary buds cultivated in vitro. HortSci. 26: 1083

    Google Scholar 

  • Huetteman CA & Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105-119

    Google Scholar 

  • Hutchinson IF & Zimmerman RH (1987) Tissue culture of temper-ate fruit and nut trees. Hort. Rev. 9: 273-349

    Google Scholar 

  • Itai A, Tanabe K, Tamura F, Susaki S, Yonemori K & Sugiura A (1995) Synthetic cytokinins control persimmon fruit shape, size and quality. J. Hort. Sci. 70: 867-873

    Google Scholar 

  • Kadota MG, Hirano T & Imizu K (2001) Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. Sci. Hort. 89: 207-215

    Google Scholar 

  • Lane WD, Iketani H & Hayashi T (1998) Shoot regeneration from cultured leaves of Japanese pear (Pyrus pyrifolia). Plant Cell Tiss. Org. Cult. 54: 9-14

    Google Scholar 

  • Liu M, Ogiwara I, Hakoda N & Shimura I (1994) Effects of BA, TDZ and CPPU on formation of adventitious shoots from callus derived from apple cotyledon. J. Jpn. Soc. Hort. Sci. 63: 505-514

    Google Scholar 

  • Lloyd G & McCown B (1981) Commercially-feasible micropropa-gation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc. Int. Plant Prop. Soc. 30: 421-427

    Google Scholar 

  • Lu C (1993) The use of thidiazuron in tissue culture. In Vitro Cell. Dev. Biol. 29: 92-96

    Google Scholar 

  • Majada JP, Sierra MI & Sanchez-Tames R (2001) Air exchange rate affects the in vitro developed leaf cuticle of carnation. Sci. Hort. 87: 121-130

    Google Scholar 

  • Matsuta N & Hirabayashi T (1989) Embryogenic cell lines from somatic embryos of grape (Vitis vinifera L.). Plant Cell Rep. 7: 684-687

    Google Scholar 

  • Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y & Okamoto T (1982) Cytokinin activity of N-phenyl-N ?-1,2,3-thiadiazol-5-ylurea (thidiazuron). Phytochemisty 21: 1509-1511

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497

    Google Scholar 

  • Nandi SK, Letham DS, Palni LMS, Wong OC & Summons RE (1989)6-Benzylaminopurine and its glycosides as naturally occurring cytokinins. Plant Sci. 61: 189-196

    Google Scholar 

  • Pawlicki N & Welander M (1994) Adventitious shoot regeneration from leaf segments of in vitro cultured shoots of the apple rootstock Jork 9. J. Hort. Sci. 69: 687-696

    Google Scholar 

  • Phan CT (1991) Vitreous state in vitro culture: ethylene versus cytokinin. Plant Cell Rep. 9: 517-519

    Google Scholar 

  • Shibli RA, Ajlouni MM, Jaradat A, Alranabi S & Shatnawi M (1997) Micropropagation in wild pear (Pyrus srica). Sci Hort. 68: 237-242

    Google Scholar 

  • Singha S (1982) Influence of agar concentration on in vitro shoot proliferation of Malus sp. ‘Almey’ and Pyrus communis "sec-kel’. J. Am. Soc. Hort. Sci. 107: 657-660

    Google Scholar 

  • Smith MAL & Obeidy AA (1991) Micropropagation of a mature, male Kentucky coffeetree. HortSci. 26: 1426

    Google Scholar 

  • Turner SR & Singha S (1990) Vitrification of crabapple, pear, and geum on gellan gum-solidified culture medium. HortSci. 25: 1648-1650

    Google Scholar 

  • van Nieuwkerk JP, Zimmerman RH & Fordham I (1986) Thidiazuron stimulation of apple shoot proliferation in vitro. HortSci. 21: 516-518

    Google Scholar 

  • Yeo DY & Reed BM (1995) Micropropagation of three Pyrus rootstocks. HortSci. 30: 620-623

    Google Scholar 

  • Yusnita S, Geneve RL & Kester ST (1990) Micropropagation of white flowering eastern redbud (Cercis canadensis var. alba L.). J. Environ. Hort. 1990: 177-179

  • Ziv M (1991) Quality of micropropagated plants vitrification. In Vitro Cell. Dev. Biol. 27: 64-69

    Google Scholar 

Download references

Author information

Author notes

  1. Masanori Kadota

    Present address: Toyama Agricultural Mutual Relief Association, 342 Anyouji, Toyama, 939-8177, Japan

Authors and Affiliations

  1. Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan

    Masanori Kadota & Yoshiji Niimi

Authors
  1. Masanori Kadota
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoshiji Niimi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masanori Kadota.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kadota, M., Niimi, Y. Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell, Tissue and Organ Culture 72, 261–265 (2003). https://doi.org/10.1023/A:1022378511659

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022378511659

Keywords

search

Navigation