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An Experimental Model of Tennis Elbow in Rats: A Study of the Contribution of the Nervous System

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This article was retracted on 27 June 2009

Abstract

The contribution of the sensory and autonomic nervous system to experimentally induced tennis elbow or lateral epicondylalgia was investigated by analyses of the release of neuropeptides from sensory (substance P, SP, neurokinin A, NKA, calcitonin gene-related peptide, CGRP) and sympathetic (neuropeptide Y, NPY) nerves. SP, CGRP, NKA, NPY-like immunoreactivity (-LI) was studied in rats cerebrospinal fluid (CSF), plasma and perfusates (PF) from the enthesis of the extensor carpi radialis brevis (ECRB) of the right elbow at 2, 6 and 24 h following 0.01 ml injection of either complete Freund adjuvans (FA) or 2% Carrageenan (CAR). The control group was injected with 0.01 ml saline. In general the changes of neuropeptide-LI in the CSF and plasma were similar for both treated groups compared with the controls, but they were more pronounced in the FA group than the CAR. SP-, NKA-, CGRP- and NPY-LI were significantly increased to a similar degree in the perfusates of the ECRBs of the treated groups with a greater increase in SP-LI in the FA than the CAR group compared with controls. When comparing the neuropeptide-LI in the CSF, plasma and PF between the 2 treated groups, there were thus few differences found. Generally a unilateral injection with either FA or CAR into the rat ECRB induced a similar alteration in the concentration of SP-, NKA-, CGRP- and NPY-LI in the CSF, plasma and PF at 2, 6 and 24 h following injection. However, the most pronounced changes in neuropeptide-LI occurred locally in the elbow ECRBs PF in both treated groups.

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REFERENCES

  1. Allender, E. 1974. Prevalence, incidence, and remission rates of some common rheumatic diseases or syndromes. Scand. J. Rheumatol. 3:145–153.

    PubMed  Google Scholar 

  2. Kivi, P. 1982. The aetiology and conservative treatment of humeral epicondylitis. Scand J Rehabil. Med. 15:37–41.

    Google Scholar 

  3. Nirschl, R. P. 1973. Tennis elbow. Orthoped. Clin. North America 4(3):787.

    Google Scholar 

  4. Haker, E. 1993. Lateral epicondylalgia: Diagnosis, treatment and evaluation. Crit Rev Phys Rehabil. Med. 5(2):129–154.

    Google Scholar 

  5. Haker, E., and T. Lundeberg. 1990. Acupuncture treatment in epicondylalgia: A comparative study of two acupuncture techniques. Clin. J. Pain 6:221–226.

    PubMed  Google Scholar 

  6. Haker, E., and Lundeberg T. 1993. Elbow band, splintage and steroids in lateral epicondylalgia. Pain Clinic 62:103–112.

    Google Scholar 

  7. Haker, E., and T. Lundeberg. 1990. Laser treatment applied to acupuncture points in lateral humeral epicondylalgia. A double-blind study. Pain 43:243–247.

    Article  PubMed  Google Scholar 

  8. Haker, E., and T. Lundeberg. 1991. Is low energy laser treatment effective in lateral epicondylalgia? J. Pain Symptom Management 6(4):241–246.

    Article  Google Scholar 

  9. Haker, E., and T. Lundeberg. 1991. Lateral epicondylalgia (tennis elbow): Report of noneffective mid-laser treatment. Arch. Phys. Med. Rehabil. 72(12):984–988.

    PubMed  Google Scholar 

  10. Haker, E., and T. Lundeberg. 1991. Pulsed ultrasound treatment in lateral epicondylalgia. A double-blind study. Scand. J. Rehabil. Med. 23:115–118.

    PubMed  Google Scholar 

  11. Burton, A. K. 1988. A comparative trial of forearm strap and topical anti-inflammatory as adjunct to manipulative therapy in tennis elbow. Man. Med. 3:141–143.

    Google Scholar 

  12. Lehman, L. 1974. Therapeutic heat and cold. Clin. Orthoped. Related Research. 99:220–237.

    Google Scholar 

  13. Devereaux, M. D., B. L. Hazleman, and P. P. Thomas. 1985. Chronic lateral humeral epicondylitis—a double blind controlled assessment of pulsed electromagnetic field therapy. Clin. Exp. Rheumatol. 3:333–336.

    PubMed  Google Scholar 

  14. Percy, E. C., and J. D. Carson. 1981. The use of DMSO in tennis elbow and rotator cuff tendonitis—a double blind study. Med. Sci. Sports Exercise. 13:215–219.

    Google Scholar 

  15. Famaey, J. P., G. Broux, D. Cleppe, A. Deroulez, F. Duckerts, and Edvard, F. et al. 1982. Ionisation with voltaren, A multi-centre trial. J. Belge. Med. Phys. Rehabil. 5(2):55–60.

    PubMed  Google Scholar 

  16. Saartok, T., and E. Eriksson. 1986. Randomized trial of oral naproxen or local injection of betamethasone in lateral epicondylitis of the humerus. Orthopedics 9(2):191–194.

    PubMed  Google Scholar 

  17. SÖlveborn, S. A., and B. Bjerking-Dickmar. 1990. Stretching as treatment of tennis elbow/radial epicondylalgia. Proc 4th Congress of the European Society for Surgery of the Shoulder and the Elbow, 1990 Oct. Milano.

  18. Chard, M. D., and B. L. Hazleman. 1989. Tennis elbow: a reappraisal. Br J Rheumatol 28:186–190.

    PubMed  Google Scholar 

  19. Chard, M. D., T. R. Cawston, G. P. Riley, G. A. Gresham, and B. L. Hazleman. 1994. Rotatorcuff degeneration and lateral epicondylitis: a comparative histological study. Ann. Rheum. Dis. 53:30–34.

    PubMed  Google Scholar 

  20. Nirschl, R. P. 1992. Elbow tendinosis/tennis elbow. Clin. Sports Med. 11(2):851–870.

    PubMed  Google Scholar 

  21. Binder, A., G. Parr, P. Thomas, and B. Hazleman. 1983. A clinical and thermography study of lateral epicondylitis. Br. J. Rheumatol. 22:77–81.

    PubMed  Google Scholar 

  22. Thomas, D., and J. P. Savage. 1989. Persistent tennis elbow: evaluation by infrared and nuclear medicine isotope scanning. Thermology 3:132–136.

    Google Scholar 

  23. Thomas, D., G. Siahamis, M. Millicent, and C. Boyle. 1992. Computerised infrared thermography and isotopic bone scanning in tennis elbow. Ann. Rheum. Dis. 51:103–107.

    PubMed  Google Scholar 

  24. Goldie, I. 1964. Epicondylitis lateralis humeri. Acta. Chir. Scand. Suppl 339.

  25. Basbaum, A. I., D. Menetrey, R. Presley, and J. D. Levine. 1988. The contribution of the nervous system to experimental arthritis in the rat. Editors. Besson, J. M., and Guibaud, G. In: The Arthritic Rat as a Model of Clinical Pain? Elsevier, Amsterdam. 41–53.

    Google Scholar 

  26. Holsapple, M. P., and G. K. W. Yim. 1983. Therapeutic reduction of ongoing carrageenin-induced inflammation by lipooxygenase, but not cyclooxygenase inhibitors. Inflammation 3:223–230.

    Google Scholar 

  27. Neil, A., J. M. Benoist, V. Kayser, and G. Guilbaud. 1987. Initial nociceptive sensitization in carrageenin-induced rat paw inflammation is dependent on amine autacoid mechanisms: electrophysiological and behavioural evidence obtained with a quaternary antihistamine, thiazinamium. Exp. Brain Res. 65:343–351.

    Article  PubMed  Google Scholar 

  28. Di Rosa, M., J. P. Giroud, and D. A. Willoughby. 1971. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J. Pathol. 104:15–29.

    PubMed  Google Scholar 

  29. Gillard, G. C., and D. A. Lowther. 1976. Carrageenin-induced arthritis. II. Effects of intraarticular injection of carrageenin on the synthesis of perprotoeoglycan in articular cartilage. Arthritis Rheum. 19:918–922.

    PubMed  Google Scholar 

  30. Carmichael D. J., G. C. Gillard, D. A. Lowther, C. J. Handley, and V. B. Santer. 1977. Carrageenin-induced arthritis. IV. Rate changes in cartilage matrix proteoglycan synthesis. Arthritis Rheum. 20:834–840.

    PubMed  Google Scholar 

  31. Lowther, D. A., J. D. Sandy, V. B. Sarter, and H. L. G. Brown. 1978. Antigen-induced arthritis. Decreased proteoglycan content and inhibition of proteoglycan synthesis in articular cartilage. Arthritis Rheum. 21:675–680.

    PubMed  Google Scholar 

  32. Konttinen, Y. T., R. Rees, M. Hukkanen, M. GrÖnblad, E. Tolvanen, S. J. Gibson, J. M. Polak, and D. A. Brewerton. 1990. Nerves in inflammatory synovium: immunohistochemical observations on the adjuvant arthritic rat model. J. Immunol. 17:1586–1591.

    Google Scholar 

  33. Bileviciute, I., T. Lundeberg, Ekblom, E. Theodorsson. 1993. Bilateral changes of substance P-, neurokinin A-, calcitonin gene-related peptide-and neuropeptidelike Y-like immunoreactivity in rat joint synovial fluid during acute monoarthritis. Neurosci. Lett. 153:37–40.

    Article  PubMed  Google Scholar 

  34. Weihe, E., D. Nohr, S. Muller, M. Buchler, H. Friess, and H.-J. Zentel. 1991. The tachykinin neuroimmune connection in inflammatory pain. Ann. N.Y. Acad. Sci. 632:283–294.

    PubMed  Google Scholar 

  35. Schaible, H.-G., B. Jarrot, P. J. Hope, and A. W. Duggan. 1990. Release of immunoreactive substance P in the spinal cord during development of acute arthritis in the knee joint of the cat. Brain Res. 529:214–223.

    Article  PubMed  Google Scholar 

  36. Hope, P. J., B. Jarrott, H.-G. Schaible, E. W. Clarke, and A. W. Duggan. 1990. Release and spred of immunoreactive neurokinin A in the cat spinal cord in a model of acute arthritis. Brain Res. 533:292–299.

    Article  PubMed  Google Scholar 

  37. Lam, F., and W. R. Ferell. 1989. Inhibition of carrageenan induced inflammation in the rat knee joint by substance P antagonist. Ann. Rheum. Dis. 48:928–932.

    PubMed  Google Scholar 

  38. Thedorsson-Norheim, E., A. Hemsen, E. Brodin, J. M. Lundberg. 1987. Sample handling techniques when analysing regulatory peptides. Life Sci. 41:845–848.

    Article  PubMed  Google Scholar 

  39. Brodin, E., N. Lindefors, C.-J. Dalsgaard, E. Theodorsson-Norheim, and S. Rosell. 1986. Tachykinin multiplicity in the rat central nervous system as studied using antisera against substance P and neurokinin A. Regul. Pept. 13:253–272.

    Article  PubMed  Google Scholar 

  40. Theodorsson-Norheim, E., R. Brodin, I. Norheim, and S. Rosell. 1984. Antisera raised against eledosin and kassinin detect immunoreactive material in rat tissue extracts: tissue distribution and chromatographic characterization. Regul. Pept. 9:229–244.

    Article  PubMed  Google Scholar 

  41. Theodorsson-Norheim, E., A. Hemsen, and J. M. Lundberg. 1985. Radioimmunoassay for neuropeptide Y (NPY): chromatographic characterization of immunoreactivity in plasma and tissue extracts. Scand. J. Clin. Lab. Invest. 45:355–365.

    PubMed  Google Scholar 

  42. Gamse, R., M. Posch, A. Saria, and G. Jancso. 1987. Several mediators appear to interact in neurogenic inflammation. Acta. Physiol. Hung. 69:343–354.

    PubMed  Google Scholar 

  43. Beck, P. W., and H. O. Handwerker. 1974. Bradykinin and serotonin effects on various types of cutaneous nerve fibres. Pflugers Arch. 347:209–222.

    PubMed  Google Scholar 

  44. Chahl, L. A., and A. Iggo. 1977. The effects of bradykinin and prostaglandin E-1 on rat cutaneous afferent nerve activity. Br. J. Pharmacol. 59:343–347.

    PubMed  Google Scholar 

  45. Birch, P. J., S.-M. Harrison, A. G. Hayes, H. Rogers, and M. B. Tyers. 1992. The non-peptide NK-1 receptor antagonist, (+)-CP-96, 345, produces antinociceptive and anti-oedema effects in the rat. Br. J. Pharmacol. 105:508–510.

    PubMed  Google Scholar 

  46. Woolf, C. J., and Z. Wiesenfeld-Hallin. 1986. Substance P and calcitonon gene-related peptide synergistically modulate the gain of the nociception reflex in the rat. Neurosci. Lett. 66:226–230.

    Article  PubMed  Google Scholar 

  47. Louis, S. M., D. Johnstone, A. J. Millest, N. J. W. Russell, and G. J. Dockray. 1990. Immunization with calcitonin gene-related peptide reduces the inflammatory response to adjuvant arthritis in the rat. Neuroscience 39:729–731.

    Article  Google Scholar 

  48. Satoh, M., Y. Kuraishi, and M. Kawamura. 1992. Effects of intrathecal antibodies to substance P, calcitonin gene-related peptide and galanin on repeated cold stress-induced hyperalgesia: comparison with carrageenan-induced hyperalgesia. Pain 49:273–278.

    Article  PubMed  Google Scholar 

  49. Thedorsson-Norheim, E., I. Norheim, K. Öberg, E. Brodin, J. M. Lundberg, K. Tatemoto, and P. G. Lindgren. 1985. Neuropeptide K: a major tachykinin in plasma and tumor tissues from carcinoid patients. Bichem. Biophys. Res. Commu. 131:77–83.

    Google Scholar 

  50. Gamse, R., M. Posch, A. Saria, and G. Jancso. 1987. Several mediators appear to interact in neurogenic inflammation. Acta. Physiol. Hung. 69:343–354.

    PubMed  Google Scholar 

  51. Wimalawansa, S. J., and I. Macintyre. 1988. Heterogenicity of plasma calcitonin gene-related peptide: partial characterization of immunoreactive forms. Peptides 407–410.

  52. Ekblad, E., L. Edvinsson, C. Wahlestedt, R. Uddman, R. HÅkansson, and F. Sundler. 1984. Neuropeptide Y co-exists and co-operates with noradrenaline in perivascular nerve fibers. Regul. Pept. 8:225–35.

    Article  PubMed  Google Scholar 

  53. Morris, M., V. Kapoor, and J. Chalmers. 1987. Plasma neuropeptide Y concentration is increased after hemorrhage in conscious rats: relative contributions of sympathetic nerves and the adrenal medulla. J. Cardiovasc. Dis. 9:541–545.

    Google Scholar 

  54. ErdÖs, E. G., and R. A. Skidgel. 1989. Neutral endopeptidase 24.11 (enkephalinase) and related regulators of peptides hormones. FASEB 3:145–151.

    Google Scholar 

  55. Nadel, J. A. 1991. Neutral endopeptidase modulates neurogenic inflammation. Eur. Respir. J. 4:745–754.

    PubMed  Google Scholar 

  56. Wimalawansa, S. J. 1990. Effects of in vivo stimulation on molecular forms of circulatory calcitonin and calcitonin gene-related peptide in man. Mol. Cell Endocrinol. 71:13–29.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Physical Therapy and Physiology and, Pharmacology Karolinska Institutet, Stockholm

    Eva Haker

  2. Department of Clinical Chemistry, University Hospital, Linköping, Sweden

    Elvar Theodorsson

  3. Department of Physiology and, Pharmacology Karolinska Institutet, Stockholm.

    Thomas Lundeberg

  4. Department of Physical Medicine and Rehabilitation, Karolinska Hospital, Stockholm, Sweden

    Thomas Lundeberg

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  1. Eva Haker
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  2. Elvar Theodorsson
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An erratum to this article is available at http://dx.doi.org/10.1007/s10753-009-9133-3.

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Haker, E., Theodorsson, E. & Lundeberg, T. An Experimental Model of Tennis Elbow in Rats: A Study of the Contribution of the Nervous System. Inflammation 22, 435–444 (1998). https://doi.org/10.1023/A:1022377001084

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