Skip to main content
SpringerLink
Log in

Putrescine Effect on Nitrate Reductase Activity, Organic Nitrogen, Protein, and Growth in Heavy Metal and Salinity Stressed Mustard Seedlings

  • Published:
Biologia Plantarum

Abstract

Putrescine effect on nitrate reductase activity, organic nitrogen and protein contents, and plant growth under Cd or Pb (0.1 – 2 mM) and salinity (5 and 100 mM NaCl) stresses was examined in Indian mustard (Brassica juncea L. cv. RH-30) seedlings. Cd or Pb and salinity inhibited nitrate reductase activity and decreased organic nitrogen and protein contents in leaf tissue. The increased nitrate reductase activity induced by putrescine was correlated with increased organic nitrogen and protein contents and growth of plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Balint, R., Cooper, G., Staebell, M., Filner, P.: N-Caffeoyl-4-amino-butyric acid, a new flower-specific metabolite in cultured tobacco cells and tobacco plants.-J. biol. Chem. 262: 11026–11031, 1987.

    Google Scholar 

  • Bouchereau, A., Aziz, A., Larher, F., Martin-Tanguy, J.: Polyamines and environmental challenges: recent development.-Plant Sci. 140: 103–125, 1999.

    Google Scholar 

  • Burzynski, M., Grabowski, A.: Influence of lead on nitrate uptake and reduction in cucumber seedlings.-Acta Soc. Bot. Pol. 53: 77–86, 1984.

    Google Scholar 

  • Galston, A.W., Kaur-Sawhney, R.: Polyamines in plant physiology.-Plant Physiol. 94: 406–410, 1990.

    Google Scholar 

  • Kakkar, R.K., Bhaduri, S., Rai, V.K., Kumar, S.: Amelioration of NaCl stress by arginine in rice seedlings: changes in endogenous polyamines.-Biol. Plant. 43: 419–422, 2000.

    Google Scholar 

  • Lang, C.A.: Simple microdetermination of Kjeldahl nitrogen in biological materials.-Anal. Chem. 30: 1692–1694, 1958.

    Google Scholar 

  • Leidi, E.O., Silberbush, M., Soares, M.M., Lips, S.H.: Salinity and nitrogen nutrition studies on peanut and cotton plants.-J. Plant Nutr. 15: 591–604, 1992.

    Google Scholar 

  • Mansour, M.M.F.: Nitrogen containing compounds and adaptation of plants to salinity stress.-Biol. Plant. 43: 491–500, 2000.

    Google Scholar 

  • Mishra, S.N., Bhutani, S., Singh, D.B.: Influence of nitrate supply on cadmium toxicity in Brassica juncea during early seedling growth.-Indian J. Plant Physiol. 37: 12–16, 1994.

    Google Scholar 

  • Mishra, S.N., Sharma, I.: Putrescine as growth inducer and nitrogen source for mustard seedling grown under salinity.-Indian J. exp. Biol. 32: 916–918, 1994.

    Google Scholar 

  • Mishra, S.N., Srivastava, H.S: Increase in nitrate reductase activity, organic nitrogen and protein content of maize leaves supplied with nitrate or ammonium.-Z. Pflanzen physiol. 113: 91–93, 1983.

    Google Scholar 

  • Shankar, N., Khan, S.R., Srivastava, H.S.: The response of nitrate reductase activity and nitrate assimilation in maize roots to growth regulators at acidic pH.-Biol. Plant. 44: 599–601, 2001.

    Google Scholar 

  • Sharma, D.P., Singh, K.N., Rao, K.V.G.K.: Response of wheat (Triticum aestivum), Indian mustard (Brassica juncea) and barley (Hordeum vulgare) to soil salinity in field.-Indian J. agr. Sci. 60: 588–591, 1990.

    Google Scholar 

  • Singh, D.N., Srivastava, H.S., Singh, R.P.: Nitrate assimilation in pea leaves in the presence of cadmium.-Water Air Soil Pollut. 42: 1–5, 1988.

    Google Scholar 

  • Sinha, S.K., Srivastava, H.S., Mishra, S.N.: Nitrate assimilation in intact and excised maize leaves in the presence of lead.-Bull. Environ. Cont. Toxicol. 41: 419–426, 1988a.

    Google Scholar 

  • Sinha, S.K., Srivastava, H.S., Mishra, S.N.: Effect of lead on nitrate reductase activity and nitrate assimilation in pea leaves.-Acta Soc. Bot. Pol. 57: 457–463, 1988b.

    Google Scholar 

  • Smith, T.A.: Polyamines.-Annu. Rev. Plant Physiol. 36: 117–143, 1985.

    Google Scholar 

  • Srivastava, H.S.: Distribution of nitrate reductase in ageing bean seedlings.-Plant Cell Physiol. 26: 995–999, 1975.

    Google Scholar 

  • Srivastava, H.S.: Multiple functions and forms of higher plant nitrate reductase.-Phytochemistry. 31: 2941–2947, 1992.

    Google Scholar 

  • Stevens, D.L., Oaks, A.: The influence of nitrate on the induction of nitrate reductase in maize roots.-Can. J. Bot. 51: 1255–1258, 1973.

    Google Scholar 

  • Sweby, D.L., Huckett, B.I., Watt, M.P.: Effect of nitrogen nutrition on salt stressed Nicotiana tabacum var. Samsun in vitro plantlets.-J. exp. Bot. 45: 995–1008, 1994.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bio-Sciences, M.D. University, Rohtak, 124001, India

    D.B. Singh, S. Varma & S.N. Mishra

Authors
  1. D.B. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Varma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S.N. Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Singh, D., Varma, S. & Mishra, S. Putrescine Effect on Nitrate Reductase Activity, Organic Nitrogen, Protein, and Growth in Heavy Metal and Salinity Stressed Mustard Seedlings. Biologia Plantarum 45, 605–608 (2002). https://doi.org/10.1023/A:1022360612976

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022360612976

Search

Navigation