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Effect of NaCl and Proline on Bean Seedlings Cultured in vitro

  • Published:
Biologia Plantarum

Abstract

Effects of NaCl (150 mM), proline (10 mM) and their combination on growth and contents of chlorophyll, proline and protein of bean (Phaseolus vulgaris cv. Kizilhaç) seedlings in vitro were investigated. NaCl decreased seedling growth. Proline added to control seedlings did not change seedling growth but decreased chlorophyll and increased protein contents. When proline added to NaCl-treated seedlings growth was increased in comparison with NaCl-treated only. Thus, proline alleviated salinity stress in bean seedlings.

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References

  • Ahmad, I., Larher, F., Mann, A.F., McNally, S.F., Stewart, G.R.: Nitrogen metabolism of halophytes. IV. Characteristics of glutamine synthetase from Triglochin maritima.-New Phytol. 91: 585–595, 1982.

    Google Scholar 

  • Arnon, D.L.: Copper enzymes in isolated chloroplasts PPO in Beta vulgaris.-Plant Physiol. 24: 1–15, 1949.

    Google Scholar 

  • Bates, L.S.; Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies.-Plant Soil 39: 205–207, 1973.

    Google Scholar 

  • Bernard, R., Oaks, A.: Metabolism of proline in maize root tips.-Can. J. Bot. 48: 1155–1158, 1969.

    Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantites of protein utulizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    Google Scholar 

  • Fukutoku, Y., Yamada, Y.: Sources of prolin-nitrogen in water stressed soybean (Glycine max). II. Fate of 15N-labelled protein.-Physiol. Plant. 61: 622–628, 1984.

    Google Scholar 

  • Hanson, A.D., Hitz, W.D.: Metabolic responses of mesophytes to plant water deficits.-Annu. Rev. Plant Physiol. 33: 163–203, 1982.

    Google Scholar 

  • Hurkman, W.J., Tanaka, C.K., Dupont, F.M.: The effects of salt stress on polypeptides in membrane fractions from barley roots.-Plant Physiol. 88: 1263–1273, 1988.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.

    Google Scholar 

  • Pandey, R., Ganapathy, P.S.: The proline enigma: NaCl-tolerant and NaCl-sensitive callus lines of Cicer arietinum.-Plant Sci. 401: 13–17, 1985.

    Google Scholar 

  • Schobert, B., Tschesche, H.: Unusual solution properties of proline and its interaction with proteins.-Biochem. biophys. Acta 541: 270–277, 1978.

    Google Scholar 

  • Stewart, G.R., Lee, J.A.: The role of proline accumulation in halophytes.-Planta 120: 279–289, 1974.

    Google Scholar 

  • Tipirdamaz, R., Çakirlar, H.: Effects of salt and water stress on proportionally water content, proline and betain in two wheat (Triticum aestivum L.) varieties grown in Turkey.-Doğa-Tr. J. Biol. 14: 125–148, 1990.

    Google Scholar 

  • Tipirdamaz, R., Karakullukçu, Ş.: Effects of proline and glycinebetaine on growth and some internal solute changes of cultured tomato embryos in salt conditions.-Doğa-Tr. J. Bot. 17: 57–64, 1993.

    Google Scholar 

  • Wyn Jones, R.G., Storey, R.: Salt stress and comparative physiology in the Gramineae. IV. Comparison of salt stress in Saptina tawnsendri and three barley cultivars.-Aust. J. Plant Physiol. 5: 839–850, 1978.

    Google Scholar 

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Demir, Y., Kocaçalişkan, I. Effect of NaCl and Proline on Bean Seedlings Cultured in vitro . Biologia Plantarum 45, 597–599 (2002). https://doi.org/10.1023/A:1022343101727

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  • DOI: https://doi.org/10.1023/A:1022343101727

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