Abstract
Effects of NaCl (150 mM), proline (10 mM) and their combination on growth and contents of chlorophyll, proline and protein of bean (Phaseolus vulgaris cv. Kizilhaç) seedlings in vitro were investigated. NaCl decreased seedling growth. Proline added to control seedlings did not change seedling growth but decreased chlorophyll and increased protein contents. When proline added to NaCl-treated seedlings growth was increased in comparison with NaCl-treated only. Thus, proline alleviated salinity stress in bean seedlings.
References
Ahmad, I., Larher, F., Mann, A.F., McNally, S.F., Stewart, G.R.: Nitrogen metabolism of halophytes. IV. Characteristics of glutamine synthetase from Triglochin maritima.-New Phytol. 91: 585–595, 1982.
Arnon, D.L.: Copper enzymes in isolated chloroplasts PPO in Beta vulgaris.-Plant Physiol. 24: 1–15, 1949.
Bates, L.S.; Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies.-Plant Soil 39: 205–207, 1973.
Bernard, R., Oaks, A.: Metabolism of proline in maize root tips.-Can. J. Bot. 48: 1155–1158, 1969.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantites of protein utulizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Fukutoku, Y., Yamada, Y.: Sources of prolin-nitrogen in water stressed soybean (Glycine max). II. Fate of 15N-labelled protein.-Physiol. Plant. 61: 622–628, 1984.
Hanson, A.D., Hitz, W.D.: Metabolic responses of mesophytes to plant water deficits.-Annu. Rev. Plant Physiol. 33: 163–203, 1982.
Hurkman, W.J., Tanaka, C.K., Dupont, F.M.: The effects of salt stress on polypeptides in membrane fractions from barley roots.-Plant Physiol. 88: 1263–1273, 1988.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.
Pandey, R., Ganapathy, P.S.: The proline enigma: NaCl-tolerant and NaCl-sensitive callus lines of Cicer arietinum.-Plant Sci. 401: 13–17, 1985.
Schobert, B., Tschesche, H.: Unusual solution properties of proline and its interaction with proteins.-Biochem. biophys. Acta 541: 270–277, 1978.
Stewart, G.R., Lee, J.A.: The role of proline accumulation in halophytes.-Planta 120: 279–289, 1974.
Tipirdamaz, R., Çakirlar, H.: Effects of salt and water stress on proportionally water content, proline and betain in two wheat (Triticum aestivum L.) varieties grown in Turkey.-Doğa-Tr. J. Biol. 14: 125–148, 1990.
Tipirdamaz, R., Karakullukçu, Ş.: Effects of proline and glycinebetaine on growth and some internal solute changes of cultured tomato embryos in salt conditions.-Doğa-Tr. J. Bot. 17: 57–64, 1993.
Wyn Jones, R.G., Storey, R.: Salt stress and comparative physiology in the Gramineae. IV. Comparison of salt stress in Saptina tawnsendri and three barley cultivars.-Aust. J. Plant Physiol. 5: 839–850, 1978.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Demir, Y., Kocaçalişkan, I. Effect of NaCl and Proline on Bean Seedlings Cultured in vitro . Biologia Plantarum 45, 597–599 (2002). https://doi.org/10.1023/A:1022343101727
Issue Date:
DOI: https://doi.org/10.1023/A:1022343101727