Plant and Soil

, Volume 248, Issue 1–2, pp 285–295 | Cite as

Plantago lanceolata L. and Rumex acetosella L. differ in their utilisation of soil phosphorus fractions

  • Ann-Mari Fransson
  • Ingrid M. van Aarle
  • Pål Axel Olsson
  • Germund Tyler
Article

Abstract

To establish relationships between soil phosphorus (P) fractions and leaf P, a mycorrhizal species (Plantago lanceolata L.) was compared with a typically non-mycorrhizal species (Rumex acetosella L.) in a glasshouse experiment. The plants were grown in 40 soils from non-fertilised, abandoned pastures or abandoned arable fields and leaf P concentration were found to be related to various soil P fractions after six weeks of growth. The differences in the P fractions in soil can account for a large share of the variation in leaf P concentration in both species, but the two species differed in their utilisation of P fractions. Leaf P concentration of R. acetosella was more related to extractable soil P than that of P. lanceolata. Rumex acetosella showed a higher maximum P concentration. The P fractions accounting for the largest share of the variation in leaf P concentration was the Bray 1 extractable and the weak oxalate (1 mM) extractable P, and for P. lanceolata also the Na2SO4+NaF extractable P fraction. P extracted with these methods accounted for up to 80% of the variation in P concentration in leaves of R. acetosella and 65% of the variation in leaves of P. lanceolata. More P extractable with weak oxalate, Na2SO4+NaF and strong oxalate (50 mM) was released from the soil than was taken up by the plants during the experimental period. The Bray 1 extractable P fraction, however, decreased in both unplanted and planted soils. Phosphatase release was not induced in any of the plants during the experimental period, indicating that they were not mobilising soil organic P. However, some of the methods extracted a large share of the organic P and still explained much of the variation in leaf P concentration. Mycorrhizal colonisation of P. lanceolata was inversely related to the extractable soil P. The consistently fast P uptake of R. acetosella indicates that this species have a high demand for P. The differences in P utilisation between R. acetosella and P. lanceolata could be caused by their different mycorrhizal status.

arbuscular mycorrhiza phosphatase phosphorus extraction plant nutrition unfertilised soil 

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References

  1. Bååth E and Spokes J 1989 The effect of added nitrogen and phosphorus on mycorrhizal growth response and infection in Allium schoenoprasum. Can. J. Bot. 67, 3227-3232.Google Scholar
  2. Bolan N S 1991 A critical review on the role of mycorrhizal fungi in the uptake of phosphorus by plants. Plant Soil 134, 189-207.Google Scholar
  3. Borges R and Mallarino A P 1998 Variation of early growth and nutrient content of no-till corn and soybean in relation to soil phosphorus and potassium supplies. Commun. Soil Sci. Plant Anal. 29, 2589-2605.Google Scholar
  4. Bray R H and Kurtz L T 1945 Determination of total, organic, inorganic and available form of phosphorus in soil. Soil Sci. 59, 39-45.Google Scholar
  5. Brundrett M and Kendrick B 1990 The roots and mycorrhizas of herbaceous woodland plants. I. Quantitative aspects of morphology. New Phytol. 114, 457-468.Google Scholar
  6. Caldwell M M, Dudley L M and Lilieholm B 1992 Soil solution phosphate, root uptake kinetics and nutrient acquisition: implications for a patchy soil environment. Oecologia 89, 305-309.Google Scholar
  7. Fitter A H 1995 Interpreting quantitative and qualitative characteristics in comparative analyses. J. Ecol. 83, 730.Google Scholar
  8. Fransson A-M 2001 Evaluation of oxalate/oxalic acid for extracting plant available phosphorus in unfertilised acid soils. Commun. Soil Sci. Plant Anal. 32, 2469-2484.Google Scholar
  9. Gahoonia T S, Care D and Nielsen N E 1997 Root hair and phosphorus acquisition of wheat and barley cultivars. Plant Soil 191, 181-188.Google Scholar
  10. Giovanetti M and Mosse B 1980 An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots. New Phytol. 84, 489-500.Google Scholar
  11. Harley J L and Harley E L 1987 A check-list of mycorrhiza in the British flora. New Phytol. (suppl.) 105, 1-102.Google Scholar
  12. Helal H M and Dressler A 1989 Mobilization and turnover of soil phosphorus in the rhizosphere. Z. Pflanzenernähr. Bodenk. 152, 175-180.Google Scholar
  13. Holford I C R 1997 Soil phosphorus: its measurement, and its uptake by plants. Aust. J. Soil Res. 35, 227-239.Google Scholar
  14. Hutchings M J and De Kroon H 1994 Foraging in plants: the role of morphological plasticity in resource acquisition. Adv. Ecol. Res. 25, 159-238.Google Scholar
  15. Jakobsen I 1986 Vesicular arbuscular mycorrhiza in field-grown crops. 3. Mycorrhizal infection and rates of phosphorus inflow in pea-plants. New Phytol. 104, 573-581.Google Scholar
  16. Jasper D A, Robson A D and Abbott L K 1979 Phosphorus and formation of vesicular-arbuscular mycorrhizas. Soil Biol. Biochem. 11, 501-505.Google Scholar
  17. Jayachandran K, Schwab A P and Hetrick B A D 1992 Mineralisation of organic phosphorus by VAM fungi. Soil Biol. Biochem. 24, 897-903.Google Scholar
  18. Joner E J, Van Aarle I M and Vosatka M 2000 Phosphatase activity of extra-radical arbuscular mycorrhizal hyphae: A review. Plant Soil 226, 199-210.Google Scholar
  19. Jones D L 1998 Organic acids in the rhizosphere-a critical review. Plant Soil 205, 25-44.Google Scholar
  20. Kuo S 1996 Phosphorus. In Methods of Soil Analysis. Part 3: Chemical Methods. Ed. D L Sparks. pp. 875-876. Soil Science Society of America Inc., Madison, Wisconsin.Google Scholar
  21. Marschner H 1995 Mineral Nutrition of Higher Plants. 2nd ed. Academic Press, London.Google Scholar
  22. Menge J A, Steirle D, Bagyaraj D J, Johnsson E L V and Leonard R T 1978 Phosphorus concentrations in plants responsible for inhibition of mycorrhizal infection. New Phytol. 80, 575-578.Google Scholar
  23. Murphy J and Riley J P 1962 A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta 27, 31-36.Google Scholar
  24. Olsen S R, Cole C V, Watanabe F S and Dean L A 1954 Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular no. 939. 1-19.Google Scholar
  25. Olsson P A, Bååth E and Jakobsen I 1997 Phosphorus effects on mycelium and storage structures of an arbuscular mycorrhizal fungus as studied in the soil and roots by fatty acid signatures. Appl. Environ. Microbiol. 63, 3531-3538.Google Scholar
  26. Phillips J M and Hayman D S 1970 Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. Soc. 55, 158-162.Google Scholar
  27. Ruzika J and Hansen E H 1981 Flow injection analysis. Chemical Analysis. John Wiley & Sons, New York.Google Scholar
  28. Saleque M A, Abedin M J and Bhuiyan N I 1996 Effects of moisture and temperature regimes on available phosphorus in wetland rice soils. Commun. Soil Sci. Plant Anal. 27, 2017-2023.Google Scholar
  29. Sanders F E 1975 The effect of foliar-applied phosphate on the mycorrhizal infections of onion roots. In Endomycorrhizas. Eds. F E Sanders, B Mosse and P B Tinker. pp. 261-276. Academic Press, London.Google Scholar
  30. Sanders F E and Tinker P B 1971 Mechanisms of absorption of phosphate from soil by Endogone mycorrhizas. Nature 233, 278-279.Google Scholar
  31. Schilling G, Gransee A, Deubel A, Lezovic G and Ruppel S 1998 Phosphorus availability, root exudates, and microbial activity in the rhizosphere. Z. Pflanzenernähr. Bodenk. 161, 465-478.Google Scholar
  32. Sibbesen E 1978 An investigation of the anion-exchange resin method for soil phosphate extraction. Plant Soil 50, 305-321.Google Scholar
  33. Smith S E and Read D J 1997 Mycorrhizal Symbiosis, 2nd ed. Academic Press, San Diego. pp. 605.Google Scholar
  34. Sokal R R and Rohlf F J 1995 Biometry. 3rd ed. W.H Freeman and Company, New York. pp. 356, 451-539.Google Scholar
  35. Ström L 1997 Root exudation of organic acids: importance to nutrient availability and the calcifuge and calcicole behaviour of plants. Oikos 80, 459-466.Google Scholar
  36. Tabatabai M A and Bremner J M 1969 Use of p-Nitrophenyl phosphate for assay of soil phosphatase activity. Soil. Biol. Biochem. 26, 301-307.Google Scholar
  37. Tarafdar J C and Claasen N 1988 Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatases produced by plant roots and microorganisms. Biol. Fertil. Soils 5, 308-312.Google Scholar
  38. Tarafdar J C and Marschner H 1994 Dual inoculation with Aspergillus fumigatus and Glomus mosseae enhances biomass production and nutrient uptake in wheat (Triticum aestivum L.) supplied with organic phosphorus as Na-phytate. Plant Soil 173, 97-102.Google Scholar
  39. Trolove S N, Hedley M J, Caradus J R and Mackay A D 1996 Uptake of phosphorus from different sources by Lotus pedunculatus and three genotypes of Trifolium repens. 2. Forms of phosphate utilised and acidification of the rhizosphere. Aust. J. Soil Res. 34, 1027-1040.Google Scholar
  40. Tyler G 1974 Heavy metal pollution and soil enzymatic activity. Plant Soil 41, 303-311.Google Scholar
  41. Tyler G 1996 Soil chemistry and plant distribution in rock habitats of southern Sweden. Nordic J. Bot. 16, 609-635.Google Scholar
  42. Tyler G and Olsson P A 1993 The calcifuge behaviour of Viscaria vulgaris. J. Veg. Sci. 4, 29-36.Google Scholar
  43. Tyler G and Ström L 1995 Differing organic acid exudation pattern explains calcifuge behaviour of plants. Ann. Bot. 75, 75-78.Google Scholar
  44. Van Aarle I M, Olsson P A and Söderström B 2001 Microscopic detection of phosphatase activity of saprophytic and arbuscular mycorrhizal fungi using a fluorogenic substrate. Mycologia 93, 17-24.Google Scholar
  45. Ziadi N, Simard R R, Tran T S and Allard G 2001 Soil-available phosphorus as evaluated by desorption techniques and chemical extractions.Can. J. Soil Sci.81, 167-174.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Ann-Mari Fransson
    • 1
  • Ingrid M. van Aarle
    • 2
  • Pål Axel Olsson
    • 2
  • Germund Tyler
    • 1
  1. 1.Department of Ecology, Soil-Plant Research, Ecology BuildingLund UniversityLundSweden
  2. 2.Department of Ecology, Microbial Ecology, Ecology BuildingLund UniversityLundSweden

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