Abstract
This paper reports for the first time the transient expression of areporter gene, LacZ, in the unicellular green alga Haematococcuspluvialis. By employing the micro-particle bombardment method,motilecells in the exponential phase showed transient expression oflacZ. This was detected in bombarded motile cells undertherupture-disc pressures of 3103 KPa and 4137 KPa.Transient expression of LacZ gene could not be observed in non-motile cells ofthis alga under the same transformation condition. No LacZ background was foundin either the motile cells or the non-motile cells. The study suggests apromising potential of the SV40 promoter and the lacZreporter gene in genetic engineering of unicellular green algae.
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Teng, C., Qin, S., Liu, J. et al. Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis. Journal of Applied Phycology 14, 497–500 (2002). https://doi.org/10.1023/A:1022314815045
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DOI: https://doi.org/10.1023/A:1022314815045