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Determination of Polysaccharides and Lipopolysaccharides by Spectrophotometry and Thermal-Lens Spectrometry

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Abstract

A procedure for the photometric determination of polysaccharides with phenol in a sulfuric acid solution was optimized to reduce the blank signal. The procedure was used for the thermal-lens determination of polysaccharides (heparin, dextran, plantaglucide) qualitatively reproducing the chemical properties of pyrogenic lipopolysaccharides under experimental conditions. The detection limits were found to be 2–5 μg/mL. With the use of thermal-lens spectrometry, lipopolysaccharides can be determined in water (in a model sample, pharmaceutical preparation pyrogenal) with a detection limit of 0.3 μg/mL, which is ten times lower than the minimal injected concentration of the preparation.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119899, Russia

    N. V. Orlova, M. A. Proskurnin, V. A. Samburova & P. V. Tsvetkov

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  1. N. V. Orlova
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  2. M. A. Proskurnin
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  3. V. A. Samburova
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  4. P. V. Tsvetkov
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Orlova, N.V., Proskurnin, M.A., Samburova, V.A. et al. Determination of Polysaccharides and Lipopolysaccharides by Spectrophotometry and Thermal-Lens Spectrometry. Journal of Analytical Chemistry 58, 149–151 (2003). https://doi.org/10.1023/A:1022306121359

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  • DOI: https://doi.org/10.1023/A:1022306121359

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