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L-leucine and L-alanine uptake by trout (Salmo trutta) cardiomyocytes: the effect of IGF-I and insulin

Fish Physiology and Biochemistry volume 25, pages 239–248 (2001)Cite this article

Abstract

The uptake of L-leucine and L-alanine by trout cardiomyocytes was analysed to identify the carriers involved in their incorporation into cells. The sodium independent systems L and asc, as well as the sodium dependent system ASC, were found to be present. The Vmax of these carriers was 33.0, 40.1 and 19.4 pmol amino acid mg−1 prot min−1 for L, asc and ASC, respectively, and the Km values obtained for these transport systems were 296, 269 and 349 μM amino acid. The L system had as its main substrates the large apolar branched-chain amino acids and showed stereospecificity. The asc and ASC systems carried the short-chain neutral amino acids and were not inhibited by Me-AIB. The ASC transporter also showed adaptive regulation. The effect of IGF-I and insulin on L-alanine uptake, as well as the incorporation of L-leucine into protein, were also studied. Low IGF-I concentrations (below 2.5 ng ml−1) stimulated the uptake of L-alanine and protein synthesis but this effect reverted at high IGF-I concentrations. Insulin depressed protein synthesis and did not affect L-alanine uptake.

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Authors and Affiliations

  1. Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 645, E-08028, Barcelona, Spain

    M.A. Gallardo, C. Castejón, I. Navarro, J. Blasco, J. Gutiérrez & J. Sánchez

  2. Centre de Referència i Desenvolupament en Aqüicultura de la Generalitat de Catalunya, Spain

    M.A. Gallardo, C. Castejón, I. Navarro, J. Blasco, J. Gutiérrez & J. Sánchez

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  1. M.A. Gallardo
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  2. C. Castejón
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  3. I. Navarro
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  4. J. Blasco
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  5. J. Gutiérrez
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  6. J. Sánchez
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Gallardo, M., Castejón, C., Navarro, I. et al. L-leucine and L-alanine uptake by trout (Salmo trutta) cardiomyocytes: the effect of IGF-I and insulin. Fish Physiology and Biochemistry 25, 239–248 (2001). https://doi.org/10.1023/A:1022285620321

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  • DOI: https://doi.org/10.1023/A:1022285620321

  • asc system
  • ASC system
  • fish
  • heart
  • L system
  • protein synthesis

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