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Stimulation of insulin-like growth factor-I production by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus

Fish Physiology and Biochemistry volume 25, pages 221–230 (2001)Cite this article

Abstract

We have previously reported growth-promoting effects of recombinant bovine growth hormone (rbGH) in Mozambique tilapia, Oreochromis mossambicus, after 4 weekly injections or a single injection of slow-releasing formulation (Posilac®) (Leedom et al. 2002). In order to obtain further understanding of the role of the growth hormone (GH)-insulin-like growth factor-I (IGF-I) axis in growth in the tilapia, the effects of rbGH on plasma and mRNA levels of IGF-I were examined. Plasma IGF-I levels were significantly increased after rbGH and Posilac® injections, and a significant correlation was observed between plasma IGF-I levels, body length and mass in both treatments. IGF-I mRNA levels in the liver and in the skeletal muscle were also significantly increased after rbGH and Posilac® injections, indicating that IGF-I gene expression in these tissues is under control of circulating GH. IGF-I mRNA levels in the gill were not affected by treatment. Liver IGF-I mRNA levels were significantly correlated with body length and with body mass after rbGH and Posilac® injections. These results indicate that the growth-promoting effect of rbGH in this species is mediated to a significant extent via its stimulation of hepatic production of IGF-I and the resulting increase in plasma IGF-I, and also possibly through locally produced IGF-I in the skeletal muscle, acting in a paracrine or autocrine fashion.

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Authors and Affiliations

  1. Department of Zoology and Hawaii Institute of Marine Biology, University of Hawaii, P.O. Box 1346, Kaneohe, HI , 96744, USA

    S. Kajimura, K. Uchida, T. Yada, L.G. Riley, T. Hirano & E.G. Grau

  2. Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, 113-8657, Japan

    S. Kajimura & T. Yada

  3. Animal Agricultural Group, Monsanto, St. Louis, MO , 63198, USA

    J.C. Byatt & R.J. Collier

  4. Department of Animal Science, University of Arizona, Tucson, AZ, 85721, USA

    R.J. Collier

  5. Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, 113-8657, Japan

    K. Aida

Authors
  1. S. Kajimura
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  2. K. Uchida
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  3. T. Yada
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  4. L.G. Riley
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  5. J.C. Byatt
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  6. R.J. Collier
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  7. K. Aida
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  8. T. Hirano
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  9. E.G. Grau
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Kajimura, S., Uchida, K., Yada, T. et al. Stimulation of insulin-like growth factor-I production by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus . Fish Physiology and Biochemistry 25, 221–230 (2001). https://doi.org/10.1023/A:1022268811599

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  • DOI: https://doi.org/10.1023/A:1022268811599

  • growth hormone
  • insulin-like growth factor-I
  • Oreochromis mossambicus
  • RNase protection assay
  • tilapia

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