Abstract
Vasoactive intestinal polypeptide (VIP) was isolated in two molecular forms from an extract of the gastroenteropancreatic system of the pallid sturgeon Scaphirhynchus albus (Acipenseriformes). VIP-1 was identical to the peptide previously isolated from the rainbow trout Oncorhynchus mykiss and the bowfin, Amia calva, consistent with the proposed sister group relationship of the Acipenseriformes and the Neopterygii. Sturgeon VIP-2 contained the amino acid substitution (Ala4 to Ser) at a site previously regarded as invariant. The isolation of two distinct VIP gene products provides some support for the hypothesis that S. albus, with approximately 120 chromosomes, is functionally tetraploid but the alternative view that the species is functionally diploid and the two peptides arose from a local duplication of the VIP gene within the Scaphirhynchus lineage cannot be rejected.
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REFERENCES
Agoston, D.V., Ballmann, M., Conlon, J.M., Dowe, G.H.C. and Whittaker, V.P. 1985. The isolation of neuropeptide-containing vesicles from guinea-pig ileum. J. Neurochem. 45: 398–406.
Bemis, W.E., Findeis, E.K. and Grande, L. 1997. An overview of Acipenseriformes. In: Sturgeon Biodiversity and Conservation. pp. 25–71. Edited by V.J. Birstein, J.R. Waldman and W.E. Bemis. Kluwer Academic Publishing, Dordrecht, the Netherlands.
Birstein, V.J. and DeSalle, R. 1998. Molecular phylogeny of Acipenserinae. Mol. Phylogenet. Evol. 9: 141–155.
Birstein, V.J., Doukakis, P. and DeSalle, R. 2002. Molecular phylogeny of Acipenseridae: nonmonophyly of Scaphirhynchinae. Copeia 2002: 287–301.
Birstein, V.J., Hanner, R. and DeSalle, R. 1997. Phylogeny of the Acipenseriformes: cytogenetic and molecular approaches. In: Sturgeon Biodiversity and Conservation. pp. 127–155. Edited by V.J. Birstein, J.R. Waldman and W.E. Bemis. Kluwer Academic Publishing, Dordrecht, the Netherlands.
Birstein, V.J. and Vasiliev, V.P. 1987. Tetraploid-octoploid relationships and karyological evolution in the order Acipenseriformes (Pisces): Karyotypes, nucleoli and nucleolus-organizer regions in four acipenserid species. Genetica 72: 3–12.
Chartrel, N., Wang, Y., Fournier, A., Vaudry, H. and Conlon, J.M. 1995. Frog vasoactive intestinal polypeptide and galanin: primary structures and effects on pituitary adenylate cyclase. Endocrinology 136: 3079–3086.
Conlon, J.M. 2002. The origin and evolution of peptide YY (PYY) and pancreatic polypeptide (PP). Peptides 23: 269–278.
Dimaline, R., Young, J., Thwaites, D.T., Lee, C.M., Shuttleworth, T.J. and Thorndyke, M.C. 1987. A novel vasoactive intestinal peptide (VIP) from elasmobranch intestine has full affinity for mammalian pancreatic VIP receptors. Biochim. Biophys. Acta 930: 97–100.
Dingerkus, G. and Howell, W.M. 1976. Karyotypic analysis and evidence of tetraploidy in North American paddlefish, Polyodon spathula. Science 194: 842–844.
Dryer, M.P. and Sandvol, A.J. 1993. Pallid sturgeon recovery plan. U.S. Fish and Wildlife Service, Bismark, North Dakota.
Du, B.-H., Eng, J., Hulmes, J.D., Chang, M., Pan, Y.C. and Yalow, R.S. 1985. Guinea pig has a unique mammalian VIP. Biochem. Biophys. Res. Commun. 128: 1093–1098.
Fontana, F. 1994. Chromosomal nucleolar organizer regions in four sturgeon species as markers of karyotype evolution in Acipenseriformes (Pisces). Genome 37: 888–892.
Gardiner, B.G., Maisey, J.G. and Littlewood, D.T.J. 1996. Interrelationships of basal neopterygians. In: Interrelationships of Fishes. pp. 117–142. Edited by M.L.J. Stiassny, L.R. Parenti and G.D. Johnson. Academic Press, San Diego.
Grande, L. and Bemis, W.E. 1991. Osteology and phylogenetic relationships of fossil and Recent paddlefishes (Polyodontidae) with comments on the interrelationships of Acipenseriformes. J. Vertebr. Paleontol. 11:Suppl.1: 1–121.
Grande, L. and Bemis, W.E. 1996. Interrelationships of Acipenseriformes, with comments on ‘Chondrostei’. In: Interrelationships of Fishes. pp. 85–115. Edited by M.L.J. Stiassny, L.R. Parenti and G.D. Johnson. Academic Press, San Diego.
Kim, J.B., Gadsbøll V., Whittaker, J., Barton, B.A. and Conlon, J.M. 2000. Gastroenteropancreatic hormones (insulin, glucagon, somatostatin and multiple forms of PYY) from the pallid sturgeon, Scaphirhynchus albus (Acipenseriformes). Gen. Comp. Endocrinol. 120: 353–363.
Krieger, J., Fuerst, P.A. and Cavender, T.M. 2000. Phylogenetic relationships of the North American sturgeons (order Acipenseriformes) based on mitochondrial DNA sequences. Mol. Phylogenet. Evol. 16: 64–72.
Larhammar, D. and Risinger, C. 1994. Molecular genetic aspects of tetraploidy in the common carp Cyprinus carpio. Mol. Phylogenet. Evol. 3: 59–68.
Li, Z., Smith, M.P., Duff, D.W., Barton, B.A., Olson, K.R. and Conlon, J.M. 1998. Purification and cardiovascular activity of [Met1,Met5]-bradykinin from the plasma of a sturgeon (Acipenseriformes). Peptides 19: 635–641.
Ludwig, A., Belfiore, N.M., Pitra, C., Svirsky, V. and Jenneckens, I. 2001. Genome duplication events and functional reduction of ploidy levels in sturgeon (Acipenser, Huso and Scaphirhynchus). Genetics 158: 1203–1215.
Nicole, P., Rouyer-Fessard, C., Couvineau, A., Drouot, C., Fulcrand, P., Martinez, J. and Laburthe, M. 2000. Alanine scanning of VIP. Structure-function relationship for binding to human recombinant VPAC1 receptor. Ann. NY Acad. Sci. 921: 352–356.
Nilsson, A. 1975. Structure of the vasoactive intestinal octacosapeptide from chicken intestine: The amino acid sequence. FEBS Lett. 60: 322–326.
Rusakov, Y.I., Moriyama, S., Bondareva, V.M., Kolychev, A.P., Amemiya, Y., Yasuda, A. and Kawauchi, H. 1998. Isolation and characterization of insulin in Russian sturgeon (Acipenser guldenstaedti). J. Peptide Res. 51: 395–400.
Tagliavini, J., Williot, P., Congiu, L., Chicca, M., Lanfredi, M., Rossi, R. and Fontana, F. (1999). Molecular cytogenetic analysis of the karyotype of the European Atlantic sturgeon, Acipenser sturio. Heredity 83: 520–525.
Thwaites, D.T., Young, J., Thorndyke, M.C. and Dimaline, R. 1989. The isolation and chemical characterization of a novel vasoactive intestinal peptide-related peptide from a teleost fish, the cod, Gadus morhua. Biochim. Biophys. Acta 999: 217–230.
Wagner, A. 1998. The fate of duplicated genes: loss or new function. BioEssays 20: 785–788.
Wang, Y., Barton, B.A., Nielsen, P.F. and Conlon, J.M. 1999a. Tachykinins (substance P and neuropeptide γ from the brains of the pallid sturgeon, Scaphirhynchus albus and the paddlefish, Polyodon spathula (Acipenseriformes). Gen. Comp. Endocrinol. 116: 21–30.
Wang, Y., Barton, B.A., Thim, L., Nielsen, P.F. and Conlon, J.M. 1999b. Purification and characterization of galanin and scyliorhinin I from the hybrid sturgeon Scaphirhynchus platorynchus × Scaphirhynchus albus (Acipenseriformes). Gen. Comp. Endocrinol. 113: 38–45.
Wang, Y. and Conlon, J.M. 1993. Neuroendocrine Peptides (NPY, GRP, VIP, somatostatin) from the brain and stomach of the alligator. Peptides 14: 573–579.
Wang, Y. and Conlon, J.M. 1995. Purification and structural characterization of vasoactive intestinal polypeptide (VIP) from the trout and bowfin. Gen. Comp. Endocrinol. 98: 94–101.
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Kim, J., Barton, B. & Conlon, J. Characterization of two molecular forms of vasoactive intestinal polypeptide (VIP) from the pallid sturgeon, Scaphirhynchus albus (Acipenseriformes). Fish Physiology and Biochemistry 25, 231–238 (2001). https://doi.org/10.1023/A:1022243304391
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DOI: https://doi.org/10.1023/A:1022243304391
- Acipenseridae
- polyploidy
- phylogeny
- Scaphirhynchinae
- structure-activity
- tetraploidization