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Study of stability of variants of ovine prions with different susceptibilities to scrapie

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Abstract

Well-defined set of sheep PrP polymorphisms at positions 136, 154 and 171 define susceptibility to scrapie, ranging from very high susceptibility observed for V136-R154-Q171 (VRQ) variant to resistance for A136-R154-R171 (ARR).

To gain insight into the mechanisms of scrapie susceptibility/resistance, the unfolding pathways of the sheep prion protein variants were analysed by differential scanning calorimetry over a wide range of pH. Thermal unfolding occurs, in the 5.0 to 6.0 pH range, through a reversible one-step process while at pH<4.5 and >6.0 unfolding intermediates are formed, which are stable in the 65–80°C range. The observed differences correlate with ovine susceptibility to scrapie.

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Authors and Affiliations

  1. Virologie et Immunologie Moléculaires (VIM), INRA, F-78352, Jouy-en-Josas, France

    H. Rezaei & J. Grosclaude

  2. INRA, Laboratoire d'Etude des Interactions des Molécules Alimentaires (LEIMA), F-44316, Nantes, France

    Y. Choiset & T. Haertl

  3. Institut de Biologie Physico-Chimique, Institut National de la Recherche Agronomique (INRA) UC 806/EA 2703 Muséum National d'Histoire Naturelle, 13, rue P. et M. Curie, F-75005, Paris, France

    P. Debey

Authors
  1. H. Rezaei
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  2. Y. Choiset
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  3. P. Debey
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  4. J. Grosclaude
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  5. T. Haertl
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Correspondence to T. Haertl.

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Rezaei, H., Choiset, Y., Debey, P. et al. Study of stability of variants of ovine prions with different susceptibilities to scrapie. Journal of Thermal Analysis and Calorimetry 71, 237–247 (2003). https://doi.org/10.1023/A:1022243006871

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  • DOI: https://doi.org/10.1023/A:1022243006871

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