Skip to main content
SpringerLink
Log in

Development of suitable protocol to overcome hyperhydricity in carnation during micropropagation

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Hyperhydricity during micropropagation of carnation (Dianthus caryophyllusL.) was reduced by media modifications. Three commercial varieties (White sim, Exquisite and Scania) tested, varied in optimal growth without hyperhydricity. Increased concentration of iron and/or magnesium reduced hyperhydricity with 0.7–0.8% agar. At some concentrations, hyperhydricity was reduced to 0% and shoot multiplication was increased. All nonhyperhydrified micropropagated plantlets survived in a glasshouse during acclimatization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Choudhary ML, Prakash ML & Prakash P (1993) Effect of different levels of agar and MS macro salts on the production of hardened carnation in vitro. Adv. Hort. Forest. 3: 165-169

    Google Scholar 

  • De Proft M, Maene J & Debergh P (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of magnolia cul-tured in vitro. Physiol. Plant. 65: 375-379

    Google Scholar 

  • Donelly VA & Vidaver W (1984) Leaf anatomy of red raspberry transferred from culture to soil. J. Am. Soc. Hort. Sci. 109: 172-176

    Google Scholar 

  • Fontes MA, Otoni WC, Carolino SMB, Brommonschenkel SH, Fontes EPB, Fari M & Louro RP (1999) Hyperhydricity in pepper plants regenerated in vitro: involvement of BiP (Binding Protein) and ultrastructural aspects. Plant. Cell. Rep. 19: 81-87

    Google Scholar 

  • Gill APS & Arora JS (1988) Performance of sim carnations under subtropical climatic conditions of Punjab. Indian. J. Hort. 45: 329-335

    Google Scholar 

  • Jain A, Husain H & Kothari SL (1997) Micro propagation of Dianthus caryophyllus L. - control of vitrification. J. Plant. Biochem. Biotechnol. 6: 35-37

    Google Scholar 

  • Kevers C, Coumans M, Coumans-gilles MF & Gasper T (1984) Physiological and biochemical events leading to vitrification of plants cultured in vitro. Physiol. Plant. 61: 69-74

    Google Scholar 

  • Kevers C & Gasper T (1986) Vitrification of carnation in vitro: change in water contents, extracellular space, air volume and ion levels. Physiol. Veg. 24: 647-653

    Google Scholar 

  • Kim KW, Byun MS & Kang MS (1988) Effect of ABA and agar in preventing vitrification of carnation plantlets cultured in vitro. J. Kor. Soc. Hort. Sci. 29: 208-215

    Google Scholar 

  • Li Y, Wang L, Ye M, Shen D, Li Y, Wang LH, Ye MM & Shen DL (1997) The factors influencing vitrification of tissue-cultured carnation plantlets. Plant. Physiol. Commun. 33: 256-258

    Google Scholar 

  • Miller RM, Kaul V, Hutchinson SF & Richards D (1991) Adventiti-ous shoot regeneration in carnation (Dianthus caryophyllus) from axillary bud explants. Ann. Bot. 67: 35-42

    Google Scholar 

  • Mujib A & Pal AK (1995) Inter varietal variation in response to in vitro cloning of carnation. Crop. Res. Hisar. 10: 190-194

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473-497

    Google Scholar 

  • Olmos E & Hallin E (1998) Ultrastructural differences of hy-perhydric and normal leaves from regenerated carnation plants. Sci. Hort. 75: 91-101

    Google Scholar 

  • Paek KY, Han BH & Choi SL (1991) Physiological, biochemical and morphological characteristics of vitrified shoots regenerated in vitro. Kor. J. Plant. Tiss. Cult. 3: 151-162

    Google Scholar 

  • Staby GI, Robertson JL, Kiplinger DC & Conover CA (1978) Chain of Life. Ohio Florists Assoc, Ohio State University, Columbus

    Google Scholar 

  • Tsay H, Tsay HS & Drew RA (1998) Effect of medium compositions at different recultures on vitrification of carnation (Dianthus caryophyllus) in vitro shoot proliferation. Acta. Hort. 461: 243-249

    Google Scholar 

  • Wetzstein HY & Sommer HE (1982) Leaf anatomy of tissue cultured Liquidambar stryraciflua during acclimatization. Am. J. Bot. 69: 1579-1586

    Google Scholar 

  • Ziv M (1991) Vitrification: Morphological and physiological dis-orders of in vitro plants. In: Debergh PC & Zimmerman RH (eds) Micropropagation: Technology and Application (pp. 45-69). Kluwer Academic Press, Dordrecht, The Netherlands

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology & Genetic Engineering, India

    Manoj K. Yadav & G.K. Garg

  2. Department of Biochemistry, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, (Uttaranchal), 263 145, India requests for offprints

    A.K. Gaur

Authors
  1. Manoj K. Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A.K. Gaur
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G.K. Garg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yadav, M.K., Gaur, A. & Garg, G. Development of suitable protocol to overcome hyperhydricity in carnation during micropropagation. Plant Cell, Tissue and Organ Culture 72, 153–156 (2003). https://doi.org/10.1023/A:1022236920605

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022236920605

Keywords

Search

Navigation