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Biochemical Genetics

, Volume 35, Issue 1–2, pp 59–76 | Cite as

The Glycophorin A Gene Family in Gorillas: Structure, Expression, and Comparison with the Human and Chimpanzee Homologues

  • Shen-Si Xie
  • Cheng-Han Huang
  • Marion E. Reid
  • Antoine Blancher
  • Olga O. Blumenfeld
Article

Abstract

Homologues of MN blood group antigens, encoded by members of the glycophorin A (GPA) gene family, are expressed in man, anthropoid apes, and some species of Old World monkeys. Previous studies had shown that a three-gene framework, most closely related to that in man, is present in the chimpanzee. Here we report the genomic structure, transcript map, and protein expression of the GYPA locus in gorillas. Compared to the corresponding human and chimpanzee homologues, gorilla GPA, GPB, and GPB/E genes each showed a high degree of sequence identity, with the same exon-intron organization. However, the expression of exons III, IV, or V encoding the extracellular or membrane domains of homologous glycophorins varied among the three species. Gorilla GPA and GPB/E genes were unique in that the former occurred in two allelic forms with or without the expression of exon III, whereas the latter contained one (ψ exon III) instead of two silenced exons (ψ exons III and IV). Differences from human but not chimpanzee GPA also included the presence of a hybrid M/N epitope and the absence of the sequon for N-glycosylation. Owing to the retention of a functional exon III, gorilla GPB was more similar to chimpanzee GPB than human GPB. A transspecies allele was identified in the gorilla that gave rise to the Henshaw (He)-like antigen similar to that found in man. These results provide further insight into the model for evolution of the GPA gene family, indicating that the mechanisms underlying inter- and intraspecific polymorphism of glycophorins could predate the divergence of gorillas as the consequence of gene duplication and diversification.

glycophorins gorilla evolution gene family gene expression 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Shen-Si Xie
    • 1
  • Cheng-Han Huang
    • 2
  • Marion E. Reid
    • 2
  • Antoine Blancher
    • 3
  • Olga O. Blumenfeld
    • 1
  1. 1.Department of BiochemistryAlbert Einstein College of MedicineBronx
  2. 2.New York Blood CenterLindsley F. Kimball Research InstituteNew York
  3. 3.Laboratoire d'Immunogenetique MoleculaireUniversity Paul Sabatier, Hospital PurpanToulouseFrance

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