Abstract
In Drosophila melanogaster, two new variants affecting the activity of phenoloxidase were found in natural populations at Gomel in Belorussia and at Krasnodar in Russia. Prophenoloxidases, A 1 and A 3 , in these variants had the same mobilities on native electrophoresis as the wild type. However, enzymatic activities in their activated states were much lower than in the wild type, whereas the existence of prophenoloxidase proteins was demonstrated. Egg-to-adult and relative viabilities in the variants did not decrease at temperatures between 18 and 29°C. Genetic analyses indicated that the genes showing the phenotype of variants are new alleles of Mox and Dox-3 on the second chromosome.
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REFERENCES
Asada, N., Fujimoto, K., Tanaka, M., and Ohnishi, E. (1993a). Genetic polymorphism of prophenoloxidase A1 in Drosophila melanogaster. Jpn. J. Genet. 68:219.
Asada, N., Fukumitsu, T., Fujimoto, K., and Masuda, K. (1993b). Activation of prophenoloxidase with 2-propanol and other organic compounds in Drosophila melanogaster. Insect Biochem. Mol. Biol. 23:515.
Ashida, M., and Yamazaki, H. I. (1990). Biochemistry of the phenoloxidase system in insects: With special reference of its activation. In Ohnishi, E., and Ishizaki, H. (eds.), Molting and Metamorphosis, Japan Scientific Societies Press, Tokyo, p. 239.
Domingo, A., and Marco, R. (1989). Visualization under ultraviolet light enhances 100-fold the sensitivity of peroxidase-stained blots. Anal. Biochem. 182:176.
Fujimoto, K., Masuda, K., Asada, N., and Ohnishi, E. (1993). Purification and characterization of prophenoloxidases from pupae of Drosophila melanogaster. J. Biochem. 113:285.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680.
Lindsley, D. L., and Zimm, G. G. (1992). The Genome of Drosophila melanogaster, Academic Press, San Diego.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265.
Mitchell, H. K., and Weber, U. M. (1965). Drosophila phenoloxidase. Science 148:964.
Pomerantz, S. H., and Li, J. P.-C. (1970). Tyrosinases (Hamster melanoma). Methods Enzymol. 17A:620.
Rizki, T. M., Rizki, R. M., and Belotti, R. A. (1985). Genetics of a Drosophila phenoloxidase. Mol. Gen. Genet. 201:7.
Towbin, H., Staehelin, T., and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedures and some applications. Proc. Natl. Acad. Sci. USA 76:4350.
Warner, C. K., Grell, E. H., and Jacobson, K. B. (1974). Phenol oxidase activity and the lozenge locus of Drosophila melanogaster. Biochem. Genet. 11:359.
Wright, T. R. F. (1987). The genetics of biogenic amine metabolism, sclerotization, and melanization in Drosophila melanogaster. Adv. Genet. 24:127.
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Asada, N. Genetic Variants Affecting Phenoloxidase Activity in Drosophila melanogaster . Biochem Genet 35, 41–49 (1997). https://doi.org/10.1023/A:1022208529461
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DOI: https://doi.org/10.1023/A:1022208529461