Abstract
Fall dormancy results in decumbent, slow shoot growth of alfalfa (Medicago sativa L.) in autumn and reduced shoot regrowth rates after herbage removal in summer. Although fall dormancy is used to predict alfalfa adaptation, we possess a poor understanding of the biological mechanisms underlying fall dormancy. Our objective was to examine growth and carbohydrate metabolism of suspension cell cultures derived from contrasting alfalfa cultivars that genetically differed in fall dormancy. Suspension cells were grown in B5h media containing 2% sucrose. Cells derived from fall non-dormant plants accumulated sugars more rapidly after transfer to fresh media and to higher concentrations than did cells derived from fall dormant alfalfa cultivars. Dark respiration rates of cells derived from non-dormant plants were similar to those derived from fall dormant plants when growth was limited at low cell sugar concentrations. However, both cell growth and dark respiration rates increased in cells derived from non-dormant cultivars in response to greater cell sugar concentrations. High growth rates of cells derived from rapid growing, fall non-dormant alfalfa cultivars were associated with rapid sugar uptake and higher cell respiration rates when compared to cells derived from dormant alfalfa cultivars.
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References
Atanassov A & Brown DCW (1984) Plant regeneration from suspension culture and mesophyll protoplasts of Medicago sativa L. Plant Cell Tiss. Org. Cult. 3: 149-162
Avice J-C, Ourry A, Lamaire G & Boucard J (1996) Nitrogen and carbon flows estimated by 15N and 13C pulse-chase labeling during regrowth of alfalfa. Plant Physiol. 112: 281-290
Boyce PJ & Volenec JJ (1992) Taproot carbohydrate concentrations and stress tolerance of contrasting alfalfa genotypes. Crop Sci. 32: 757-761
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle protein dye binding. Anal. Biochem. 72: 248-254
Cunningham SM & Volenec JJ (1996) Seasonal carbohydrate and nitrogen metabolism in roots of contrasting alfalfa (Medicago sativa L.) cultivars. J. Plant Physiol. 153: 220-225
Cunningham SM, Volenec JJ & Teuber LR (1998) Plant survival and root and bud composition of alfalfa populations selected for contrasting fall dormancy. Crop Sci. 38: 962-969
Cunningham SM, Gana JA, Volenec JJ & Teuber LR (2001) Winter hardiness, root physiology, and gene expression in successive fall dormancy selections from ‘Mesilla’ and ‘CUF 101’ alfalfa. Crop Sci. 41: 1091-1098
Fankhauser JJ Jr. & Volenec JJ (1989) Root vs. shoot effects on herbage regrowth and carbohydrate metabolism of alfalfa. Crop Sci. 29: 735-740
Gamborg OL, Miller NA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158
Graber LF, Nelson NT, Luekel WA & Albert WB (1927) Organic food reserves in relation to growth of alfalfa and other perennial herbaceous plants. Wis. Agric. Exp. Stn. Res. Bull 80
Hendershot KL & Volenec JJ (1989) Shoot growth, dark respiration, and non-structural carbohydrates of contrasting alfalfa genotypes. Crop Sci. 29: 1271-1275
Kalengamaliro NE, Volenec JJ, Cunningham SM & Joern BC (1997) Seedling development and deposition of starch and storage proteins in alfalfa roots. Crop Sci. 37: 1194-1200
Kalengamaliro NE, Gana JA, Cunningham SM & Volenec JJ (2000) Mechanisms regulating differential freezing tolerance of suspension cell cultures derived from contrasting alfalfa genotypes. Plant Cell Tiss. Org. Cult. 61: 143-151
Koehler LH (1952) Differentiation of carbohydrates by anthrone reaction rate and color intensity. Anal. Chem. 24: 1576-1579
McKenzie J.S. & G.E. McLean (1989) Alfalfa winter survival research in Northwestern Canada (pp. 141-150) In: Proc. First Inter. Symp. Agric. Technique for Cold Regions, 3 to 6 Sept 1989, Obihiro, Japan
Moser LE, Volenec JJ & Nelson CJ (1982) Respiration, carbohydrate content, and leaf growth of tall fescue. Crop Sci. 22: 781-786
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-479
Schwab PM, Barnes DK & Sheaffer CC (1996) The relationship between field winter injury and fall growth score for 251 alfalfa cultivars. Crop Sci. 36: 418–426
Singh Y & Winch JE (1974) Morpological development of two alfalfa cultivars under various harvesting schedules. Can. J. Plants Sci. 54: 79–87
SAS Institute (1989) SAS/STAT User's Guide. Version 6. 4th ed. Edn. SAS Institute Inc, Cary, NC
Sheaffer CC Barnes DK, Warnes DD, Lueschen WE, Ford HJ & Swanson DR (1992) Seeding-year cutting affects winter survival and its association with fall growth score in alfalfa. Crop Sci. 32: 225–231
Shibli RA, Haagenson DM, Cunningham SM, Berg WK & Volenec JJ (2000) Cryopreservation of alfalfa (Medico sativa L.) Cells by encapsulation-dehydration. Plant Cell Rep. 20: 445–450
Smith D & Silva JP (1969) Use of carbohydrate and nittrogen root reserves in the regrowth of alfalfa from greenhouse expirements under light and dark conditions. Crop Sci. 9: 464–467
Snedecor GW & Cochran WG (1980) Statistical Methods. 7th edn pp. 593 Iowa State University Press. Ames, Iowa, USA
Vegis A (1964) Dormancy in higher plants. Annu. Res. Plant Physiol. Plant Mol. Biol. 15: 185–224
Volence JJ (1985) Leaf area expansion and shoot elongation of diverse alfalfa germplams. Crop Sci. 25: 822–827
Volence JJ (1988) Herbage growth and carbohydrate metabolism of diploid and tetraploid alfalfa. Crop Sci. 28: 128–132
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Kalengamaliro, N., Cunningham, S. & Volenec, J. Growth, sugar accumulation, and dark respiration of suspension cell cultures derived from contrasting alfalfa cultivars. Plant Cell, Tissue and Organ Culture 72, 163–171 (2003). https://doi.org/10.1023/A:1022203226367
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DOI: https://doi.org/10.1023/A:1022203226367