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Clonal differences in the response of dark and light reactions of photosynthesis to elevated atmospheric CO2 in poplar

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Photosynthetica

Abstract

Two hybrid poplar (Populus) clones (i.e., fast growing clone Beauprè and slow growing clone Robusta) were grown for two years from cuttings at close spacings in open top chambers (OTCs) under ambient (AC) and elevated [EC = AC + 350 µmol(CO2) mol-1] CO2 treatments. For clone Beauprè no down-regulation of photosynthesis was observed. Two years of growing under EC resulted in an increase in quantum yield of photosystem 2 (PS2), steady state irradiance saturated rate of net photosynthesis (P Nmax), chlorophyll (Chl) content, and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC) activity for this clone. We suppose that under non-limiting conditions of nitrogen and phosphorus content the response to EC was by building up light-harvesting complexes of PS2 and increasing photochemical efficiency of PS2. Due to a high rate of the primary reactions of photosynthesis and a high RuBPCO activity the end product of the response to EC was an increase in PNmax and a larger saccharides content. The Robusta clone showed a depression in the primary reactions of photosynthesis under EC. We found a decrease in quantum yield of PS2, Chl and phosphorus contents, and in RuBPCO activity. However, an increase in PNmax, saccharides content and Chl a/b ratio was observed. We speculate (1) that the phosphorus deficiency in combination with an increase in CO2 concentrations may lead to a potential damage of the assimilation apparatus of the primary reactions of photosynthesis and to a decrease in photochemical efficiency of PS2; (2) that the primary target of "down-regulation" takes place at PS2 for irradiances above 150 µmol m-2 s-1.

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References

  • Aliev, J.A., Guliev, N.M., Kerimov, S.K., Hidayatov, R.B.: Photosynthetic enzymes of wheat genotypes differing in productivity.-Photosynthetica 32: 77–85, 1996.

    CAS  Google Scholar 

  • Beeson, R.C., Jr., Graham, M.E.D.: CO2 enrichment of greenhouses affects neither Rubisco nor carbonic anhydrase activities.-J. amer. Soc. hort. Sci. 116: 1040–1045, 1991.

    CAS  Google Scholar 

  • Besford, R.T.: Some properties of ribulose bisphosphate carboxylase extracted from tomato leaves.-J. exp. Bot. 35: 495–504, 1984.

    CAS  Google Scholar 

  • Besford, R.T.: The greenhouse effect: Acclimation of tomato plants growing in high CO2, relative changes in Calvin cycle enzymes.-J. Plant Physiol. 136: 458–463, 1990.

    CAS  Google Scholar 

  • Bolhàr-Nordenkampf, H.R., Long, S.P., Baker, N.R., Öquist, G., Schreiber, U., Lechner, E.G.: Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation.-Funct. Ecol. 3: 497–514, 1989.

    Article  Google Scholar 

  • Bowes, G.: Growth at elevated CO2: photosynthetic responses mediated through Rubisco.-Plant Cell Environ. 14: 795–806, 1991.

    Article  CAS  Google Scholar 

  • Bowes, G.: Facing the inevitable: Plants and increasing atmospheric CO2.-Annu. Rev. Plant Physiol. Plant mol. Biol. 44: 309–332, 1993.

    Article  CAS  Google Scholar 

  • Ceulemans, R., Impens, I., Steenackers, V.: Stomatal and anatomical leaf characteristics of 10 Populus clones.-Can. J. Bot. 62: 513–518, 1984.

    Google Scholar 

  • Ceulemans, R., Jiang, X.N., Shao, B.Y.: Growth and physiology of one-year old poplar (Populus) under elevated atmospheric CO2 levels.-Ann. Bot. 75: 609–617, 1995a.

    Article  CAS  Google Scholar 

  • Ceulemans, R., Mousseau, M.: Effects of elevated atmospheric CO2 on woody plants.-New Phytol. 127: 425–446, 1994.

    Article  Google Scholar 

  • Ceulemans, R., Van Praet, L., Jiang, X.N.: Effects of CO2 enrichment, leaf position and clone on stomatal index and epidermal cell density in poplar (Populus).-New Phytol. 131: 99–107, 1995b.

    Article  Google Scholar 

  • Chow, W.S., Melis, A., Anderson, J.M.: Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis.-Proc. nat. Acad. Sci. USA 87: 7502–7506, 1990.

    Article  PubMed  CAS  Google Scholar 

  • Conroy, J.P., Smillie, R.M., Küppers, M., Bevege, D.I., Barlow, E.W.: Chlorophyll a fluorescence and photosynthetic and growth responses of Pinus radiata to phosphorus deficiency, drought stress, and high CO2.-Plant Physiol. 81: 423–429, 1986.

    PubMed  CAS  Google Scholar 

  • Coombs, J., Hind, G., Leegood, R.C., Tieszen, L.L., Vonshak, A.: Analytical techniques.-In: Coombs, J., Hall, D.O., Long, S.P., Scurlock, J.M.O. (ed.): Techniques in Bioproductivity and Photosynthesis. 2nd Ed. Pp. 219–228. Pergamon Press, Oxford 1985.

    Google Scholar 

  • DeLucia, E.H., Sasek, T.W., Strain, B.R.: Photosynthetic inhibition after long-term exposure to elevated levels of atmospheric carbon dioxide.-Photosynth. Res. 7: 175–184, 1985.

    Article  CAS  Google Scholar 

  • Eamus, D., Jarvis, P.G.: The direct effects of increase in the global atmospheric CO2 concentration on natural and commercial temperate trees and forests.-Adv. ecol. Res. 19: 1–55, 1989.

    Article  Google Scholar 

  • Epron, D., Dreyer, E., Picon, C., Guehl, J.M.: Relationship between CO2-dependent O2 evolution and photosystem II activity in oak (Quercus petraea) trees grown in the field and in seedlings grown in ambient or elevated CO2.-Tree Physiol. 14: 725–733, 1994.

    PubMed  CAS  Google Scholar 

  • Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87–92, 1989.

    CAS  Google Scholar 

  • Gunderson, C.A., Wullschleger, S.D.: Photosynthetic acclimation in trees to rising atmospheric CO2: A broader perspective.-Photosynth. Res. 39: 369–388, 1994.

    Article  CAS  Google Scholar 

  • Isebrands, J.G., Ceulemans, R., Wiard, B.M.: Genetic variation in photosynthetic traits among Populus clones in relation to yield.-Plant Physiol. Biochim. 26: 427–437, 1988.

    Google Scholar 

  • Jackson, W.A., Volk, R.J.: Photorespiration.-Annu. Rev. Plant Physiol. 21: 385–432, 1970.

    Article  CAS  Google Scholar 

  • Jarvis, P.G.: Atmospheric carbon dioxide and forests.-Phil. Trans. roy. Soc. London B324: 369–392, 1989.

    Google Scholar 

  • Krause, G.H., Weis, E.: The photosynthetic apparatus and chlorophyll fluorescence. An introduction.-In: Lichtenhaler, H.K. (ed.): Applications of Chlorophyll Fluorescence in Photosynthesis Research. Stress Physiology, Hydrobiology and Remote Sensing. Pp. 3–11. Kluwer Academic Publ., Dordrecht-Boston-London 1988.

    Google Scholar 

  • Long, S.P., Baker, N.R., Raines, C.A.: Analysing the responses of photosynthetic CO2 assimilation to long-term elevation of atmospheric CO2 concentration.-Vegetatio 104/105: 33–45, 1993.

    Article  Google Scholar 

  • Marek, M.V., Kalina, J.: Comparison of two experimental approaches used in the investigations of the long-term effects of elevated CO2 concentration.-Photosynthetica 32: 129–133, 1996.

    CAS  Google Scholar 

  • Masle, J., Hudson, G.S., Badger, M.R.: Effects of ambient CO2 concentration on growth and nitrogen use in tobacco (Nicotiana tabacum) plants transformed with an antisense gene to the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase.-Plant Physiol. 103: 1075–1088, 1993.

    PubMed  CAS  Google Scholar 

  • Moran, R.: Formulae for determination of chlorophyllous pigments extracted with N,N-dimethylformamide.-Plant Physiol. 69: 1376–1381, 1982.

    PubMed  CAS  Google Scholar 

  • Overdieck, D.: Effects of elevated CO2 concentration levels on nutrient contents of herbaceous and woody plants.-In: Goudriaan, J., Van Keulen, H., Van Laar, H.H. (ed.): The Greenhouse Effect and Primary Productivity in European Agro-Ecosystems. Pp. 31–37. Pudoc, Wageningen 1990.

    Google Scholar 

  • Peterson, R.B.: Effects of O2 and CO2 concentrations on quantum yields of photosystem I and II in tobacco leaf tissue.-Plant Physiol. 97: 1388–1394, 1991.

    PubMed  CAS  Google Scholar 

  • Pettersson, R., McDonald, A.J.S.: Effects of elevated carbon dioxide concentration on photosynthesis and growth of small birch plants (Betula pendula Roth.) at optimal nutrition.-Plant Cell Environ. 15: 911–919, 1992.

    Article  CAS  Google Scholar 

  • Sage, R.F., Sharkey, T.D., Seemann, J.R.: Acclimation of photosynthesis to elevated CO2 in five C3 species.-Plant Physiol. 89: 590–596, 1989.

    PubMed  CAS  Google Scholar 

  • Sicher, R.C., Kremer, D.F., Rodermel, S.R.: Photosynthetic acclimation to elevated CO2 occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content.-Plant Physiol. 104: 409–415, 1994.

    PubMed  CAS  Google Scholar 

  • Stitt, M.: Rising CO2 levels and their potential significance for carbon flow in photosynthetic cells.-Plant Cell Environ. 14: 741–762, 1991.

    Article  CAS  Google Scholar 

  • Tissue, D.T., Thomas, R.B., Strain, B.R.: Long-term effects of elevated CO2 and nutrients on photosynthesis and rubisco in loblolly pine seedlings.-Plant Cell Environ. 16: 859–865, 1993.

    Article  CAS  Google Scholar 

  • Vu, J.C.V., Allen, J.H., Jr., Bowes, G.: Drought stress and elevated CO2 effects on soybean ribulose bisphosphate carboxylase activity and canopy photosynthetic rates.-Plant Physiol. 83: 573–578, 1983.

    Google Scholar 

  • Weis, E., Lechtenberg, D.: Fluorescence analysis during steady state photosynthesis.-Phil. Trans. roy. Soc. London B323: 253–268, 1989.

    Google Scholar 

  • Wilkins, D., Van Oosten, J.-J., Besford, R.T.: Effects of elevated CO2 on growth and chloroplast proteins in Prunus avium.-Tree Physiol. 14: 769–779, 1994.

    PubMed  CAS  Google Scholar 

  • Yelle, S., Beeson, R.C., Jr., Trudel, M.J., Gosselin, A.: Acclimation of two tomato species to high atmospheric CO2. II. Ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase.-Plant Physiol. 90: 1473–1477, 1989.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Physics, University of Ostrava, Bráfova 7, CZ-701 03 c, Ostrava, Czech Republic

    J. Kalina

  2. Department of Biology, University of Antwerpen (UIA), Universiteitsplein 1, B-2610, Wilrijk, Belgium

    R. Ceulemans

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  1. J. Kalina
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  2. R. Ceulemans
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Kalina, J., Ceulemans, R. Clonal differences in the response of dark and light reactions of photosynthesis to elevated atmospheric CO2 in poplar. Photosynthetica 33, 51–61 (1997). https://doi.org/10.1023/A:1022123204974

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