Abstract
We used restriction length polymorphism (RFLP) analysis of PCR-amplified fragments of mtDNA to study the genetic structure of chum salmon populations sampled in 1993–2000 during a spawning run in five rivers: Narva (Southern Primorye), Naiba (Sakhalin Island), Sernovodnaya (Kunashir Island, Southern Kuril Islands), Ola (northwestern coast of the Sea of Okhotsk), and Anadyr' (Chukotka Peninsula). In total, 49 haplotypes were identified in 193 fish. Heterogeneity tests showed highly significant (P = 0) differences among all sample pairs. The estimated time of independent divergence of the populations or population groups is in good agreement with the time of Pleistocene glaciations. This result suggests that it is cyclic global changes during this time period that were crucial in determining the within-species divergence in chum salmon. The types of mtDNA genetic variability and mismatch distribution between haplotypes in the populations indicate that the southern regions of the Sea of Okhotsk and Sea of Japan served as refugia for chum salmon during glaciation periods.
Similar content being viewed by others
References
Moritz, C., Bowling, T.E., and Brown, W.M., Evolution of Animal Mitochondrial DNA: Relevance for Population Biology and Systematics, Annu. Rev. Ecol. Syst., 1987, vol. 18, pp. 269-292.
Billington, N. and Hebert, P.D.N., Mitochondrial DNA Diversity in Fish and Its Implications for Introductions, Can. J. Fish. Aquat. Sci., 1991, vol. 48, pp. 80-94.
Avise, J.C., Molecular Markers, Natural History and Evolution, New York: Chapman and Hall, 1994.
Avise, J.C., Walker, D., and Johns, G.C., Speciation Durations and Pleistocene Effects on Vertebrate Phylogeography, Proc. R. Soc. London, B, 1998, vol. 265, pp. 1707-1712.
Altukhov, Yu.P., Salmenkova, E.A., and Omel'-chenko, V.T., Populyatsionnaya genetika lososevykh ryb (Population Genetics of Salmonids), Moscow: Nauka, 1997.
Varnavskaya, N.V., Principles of Genetic Identification of Pacific Salmon Populations of the Genus Oncorhynchus in Connection with the Problems of Rational Use, Doctoral (Biol.) Dissertation, Moscow: Inst. General Genetics, 2001.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989.
Gharrett, A.J., Gray, A.K., and Brykov, Vl.A., Phylogenetic Analysis of Mitochondrial DNA Variation in Alaskan Coho Salmon Oncorhynchus kisutch, Fish. Bull., 2001, vol. 99, pp. 528-544.
Churikov, D., Matsuoka, M., Luan, X., et al., Assessment of Concordance among Genealogical Reconstructions from Various mtDNA Segments in Three Species of Pacific Salmon (Genus Oncorhynchus ), Mol. Ecol., 2001, vol. 10, pp. 2329-2339.
Zaykin, D.V. and Pudovkin, A.I., Two Programs to Estimate the Significance of Χ2 Values Using Pseudo-Probability Test, J. Hered., 1993, vol. 84, p. 152.
Schneider, S., Roessli, D., and Excoffier, L., Arlequin Ver. 2.000: A Software for Population Genetic Data Analysis, Genetic and Biometric Laboratory, Switzerland: Univ. of Geneva, 2000.
McElroy, D., Moran, P., Birmingham, E., and Kornfield, I., REAP: An Integrated Environment for the Manipulation and Phylogenetic Analysis of Restriction Data, J. Hered., 1992, vol. 83, pp. 153-158.
Nei, M. and Li, W.-H., Mathematical Model for Shifting Genetic Variation in Terms of Restriction Endonucleases, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 5269-5273.
Nei, M., Molecular Evolutionary Genetics, New York: Columbia Univ. Press, 1987.
Nei, M. and Tajima, F., Maximum Likelihood of the Number of Nucleotide Substitutions from Restriction Sites Data, Genetics, 1983, vol. 105, pp. 207-217.
Rolf, F.J., NTSYS-Ps: Numerical Taxonomy and Multivariate Analysis System, Version 1.60, New York: Exter, 1990.
Martin, A.P. and Palumbi, S.R., Body Size, Metabolic Rate, Generation Time, and the Molecular Clock, Proc. Natl. Acad. Sci. USA, 1993, vol. 90, pp. 4087-4091.
Lambeck, K. and Chappel, J., Sea Level Change through the Last Glacial Cycle, Science, 2001, vol. 292, pp. 679- 685.
Zubakov, V.A. and Borzenkova, I.I., Paleoklimaty pozdnego kainozoya (Late Cainosoan Paleoclimates), Leningrad: Gidrometeoizdat, 1983.
Slatkin, M. and Hudson, R.R., Pairwise Comparisons of Mitochondrial DNA Sequences in Stable and Exponential Growing Populations, Genetics, 1991, vol. 129, pp. 555-562.
Rodgers, A.R. and Harpending, H., Population Growth Makes Waves in the Distribution of Pairwise Genetic Differences, Mol. Biol. Evol., 1992, vol. 9, pp. 552-569.
Nee, S., Holmes, E.C., and Harvey, P.H., Inferring Population History from Molecular Phylogenies, Philos. Trans. R. Soc. London, B, 1995, vol. 349, pp. 25-31.
Brykov, Vl.A., Kirillova, O.N., Kukhlevskii, A.D., et al., Analysis of the Mitochondrial DNA Variation in Calico Salmon Oncorhynchus keta (Walbaum) from Populations of Primorye and Sakhalin Rivers, Genetika (Moscow), 2000, vol. 36, no. 10, pp. 1388-1393.
Rodgers, A.R., Genetic Evidence for the Pleistocene Population Explosion, Evolution, 1995, vol. 49, pp. 608- 615.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brykov, V.A., Polyakova, N.E. & Prokhorova, A.V. Phylogeographic Analysis of Chum Salmon Oncorhynchus keta Walbaum in Asian Populations Based on mtDNA Variation. Russian Journal of Genetics 39, 61–67 (2003). https://doi.org/10.1023/A:1022074911828
Issue Date:
DOI: https://doi.org/10.1023/A:1022074911828