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Role of protein kinase C during insulin mediated skeletal muscle cell spreading

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Abstract

Protein kinase C (PKC) is known to play important roles in integrin mediated cell spreading. This study investigated the role of PKC during insulin mediated muscle cell spreading, which was independent of integrin α5. We found that PKC-α becomes active and localise to membrane during insulin mediated cell spreading. We also found that PKC activation is essential for cell spreading stimulated by insulin and this activation enhances the cell spreading. PKC activation increased the tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin as well as tyrosine kinase activity of FAK. We also observed that PKC activation enhanced the FAK associated PI 3-kinase activity and also increased the activation of ERK-1/-2. Moreover, the effect of PKC activation on insulin mediated cell spreading as well as tyrosine phosphorylation of FAK and paxillin depends upon integrity of actin cytoskeleton. Thus, PKC is an important signaling protein during insulin mediated muscle cell spreading.

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References

  • Bandyopadhyay G, Standaert ML, Zhao L, Yu B, Avignon A, Galloway L, Karnam P, Moscat J and Farese RV (1997) Activation of protein kinase C (α, β, and ς,) by insulin in 3T3/L1 cells. J Biol Chem 274: 2551-2558.

    Google Scholar 

  • Barry ST and Critchley DR (1994) The RhoA-dependent assembly of focal adhesions in swiss 3T3 cells is associated with increased tyrosine phosphorylation and the recruitment of both pp125FAK and protein kinase delta to focal adhesions. J Cell Sci 107: 2033-2045.

    PubMed  CAS  Google Scholar 

  • Burridge CA and Chrzanowska-Wodnicka M (1996) Focal adhesions, contractility, and signalling. Ann Rev Cell Dev Biol 12: 463-519.

    Article  CAS  Google Scholar 

  • Chen Q, Kinch MS, Lin TH, Burridge K and Juliano RL (1994) Integrin mediated cell adhesion activates mitogen-activated protein kinases. J Biol Chem 269: 26,602-26,605.

    CAS  Google Scholar 

  • Clark EA and Brugge JS (1995) Integrins and signal transduction pathways: the road taken. Science 268: 233-239.

    PubMed  CAS  Google Scholar 

  • Defilippi P, Venturino M, Gulino D, Duperray A, Boquet P, Fiorentini C, Volpe G, Palmieri M, Silengo L and Tarone G (1997) Dissection of pathways implicated in integrin-mediated actin cytoskeleton assembly. J Biol Chem 272: 21,726-21,734.

    Article  CAS  Google Scholar 

  • De Nichilo MO and Yamada KM (1996) Integrin αVβ5-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin. J Biol Chem 271: 11,016-11,022.

    CAS  Google Scholar 

  • Disatnik M-H and Rando TA (1999) Integrin mediated muscle cell spreading-the role of protein kinase C in outside-in and inside-out signaling and evidence of integrin cross-talk. J Biol Chem 274: 32,486-32,492.

    Article  CAS  Google Scholar 

  • Farrelly N, Lee Y, Oliver J, Dive C and Streuli CH (1999) Extracellular matrix regulates apoptosis in mammary epithelium through a control on insulin signaling. J Cell Biol 144: 1337-1347.

    Article  PubMed  CAS  Google Scholar 

  • Frisch SM and Francis H (1994) Disruption of epithelial cell-matrix interactions induces apoptosis. J Cell Biol 124: 619-626.

    Article  PubMed  CAS  Google Scholar 

  • Goel HL and Dey CS (2002) Focal adhesion kinase tyrosine phosphorylation is associated with myogenesis and modulated by insulin. Cell Prolif 35: 131-142.

    Article  PubMed  CAS  Google Scholar 

  • Haimovich B, Kaneshiki N and Ji P (1996) Protein kinase C regulates tyrosine phosphorylation of pp125FAK in platelets adherant to fibrinogens. Blood 87: 152-161.

    PubMed  CAS  Google Scholar 

  • Hayashi YK, Chou FL, Engvall E, Ogawa M, Matsuda C, Hirabayashi S, Yokochi K, Ziober BL, Kramer RH, Kaufman SJ, Ozawa E, Gotto Y, Nonaka I, Tsukahara T, Wang JZ, Hoffman EP and Arahata K (1998) Mutation in integrin α7 gene cause congenital myopathy. Nat Genet 19: 94-97.

    PubMed  CAS  Google Scholar 

  • Haller H, Lindschau C, Maasch C, Oltho. H, Kurscheid D and Luft FC (1998) Integrin-induced protein kinase Cα and Cε translocation to focal adhesions mediates vascular smooth muscle cell spreading. Circ Res 82: 157-165.

    PubMed  CAS  Google Scholar 

  • Ilic D, Almeida E, Schlaepfer D, Dazin P, Aizawa S and Damsky C (1998) Extracellular survival signals transduced by focal adhesion kinase suppress p53-mediated apoptosis. J Cell Biol 143: 547-560.

    Article  PubMed  CAS  Google Scholar 

  • Juliano RL and Haskill S (1993) Signal transduction from the extracellular matrix. J Cell Biol 120: 577-585.

    Article  PubMed  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227: 680-685.

    Article  PubMed  CAS  Google Scholar 

  • Lewis JM, Cheresh DA and Schwartz MA (1996) Protein kinase C regulates α v β 5-dependent cytoskeletal associations and focal adhesion kinase phosphorylation. J Cell Biol 134: 1323-1332.

    Article  PubMed  CAS  Google Scholar 

  • Lipfert I, Haimovich B, Schaller MD, Cobb BS, Parsons JT and Brugge JS (1992) Integrin dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets. J Cell Biol 119: 905-912.

    Article  PubMed  CAS  Google Scholar 

  • Liu J-P, Baker J, Perkins AS, Robertson EJ and Efstratiadis A (1993) Mice carrying null mutations of the genes encoding insulin-like growth factor 1 (Igf-1)& type 1 IGF receptor (Igf1r). Cell 75: 59-72.

    Article  PubMed  CAS  Google Scholar 

  • Ma A, Richardson A, Schaefer EM and Parsons JT (2001) Serine phosphorylation of focal adhesion kinase in interphase and mitosis: a possible role in modulating binding to p130Cas. Mol Biol Cell 12: 1-12.

    PubMed  CAS  Google Scholar 

  • Mayer U, Saher G, Faessler R, Bornemann A, Echtermeyer F, Von der Mark H, Miosge N, Poeschi E and Von der Mark K (1997) Absence of integrin α7 causes a novel form of muscular dystrophy. Nat Genet 17: 318-323.

    PubMed  CAS  Google Scholar 

  • Menko AS and Boettiger D (1987) Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation. Cell 51: 51-57.

    Article  PubMed  CAS  Google Scholar 

  • Newton AC (2001) Protein kinase C: structural and spatial regulation by phosphorylation, cofactors, and macromolecular interactions. Chem Rev 101: 2353-2364.

    Article  PubMed  CAS  Google Scholar 

  • Nishiya N, Tachibana K, Shibanuma M, Mashimo J and Nose K (2001) Hic-5-reduced cell spreading on bronectin: competitive effects between paxillin and Hic-5 through interaction with focal adhesion kinase. Mol Cell Biol 21: 5332-5245.

    Article  PubMed  CAS  Google Scholar 

  • Raines EW (2000) The extracellular matrix can regulate vascular cell migration, proliferation and survival: relationships to vascular disease. Int J Exp Pathol 81: 173-182.

    Article  PubMed  CAS  Google Scholar 

  • Rankin S and Rozengurt E (1994) Platelet-derived growth factor modulation of focal adhesion kinase (p125(FAK)) and paxillin tyrosine phosphorylation in swiss 3T3 cells. Bell shaped dose response and cross talk with bombesin. J Biol Chem 269: 704-710.

    PubMed  CAS  Google Scholar 

  • Renshaw MW, Price LS and Schwartz MA (1999) Focal adhesion kinase mediates the integrin signaling requirement for growth factor activation of MAP kinase. J Cell Biol 147: 611-618.

    Article  PubMed  CAS  Google Scholar 

  • Richardson A and Parsons T (1996) A mechanism for regulation of the adhesion-associated protein tyrosine kinase pp125FAK. Nature 380: 538-540.

    Article  PubMed  CAS  Google Scholar 

  • Schmitz-Peiffer C, Craig DL and Biden TJ (1999) Ceramide generation is sufficient to account for the inhibition of the insulin-stimulated PKB pathway in C2C12 skeletal muscle cells pretreated with palmitate. J Biol Chem 274: 24,202-24,210.

    Article  CAS  Google Scholar 

  • Shattil SJ, Haimovich B, Cunningham M, Lipfert L, Parsons JT, Ginsberg MH and Brugge JS (1994) Tyrosine phosphorylation of pp125FAK in platelets requires coordinated signaling through integrins and agonists receptors. J Biol Chem 269: 14,738-14,745.

    CAS  Google Scholar 

  • Shikano T, Haneda M, Togawa M and Kikkawa R (1996) Tyrosine phosphorylation of focal adhesion kinase (p125FAK) and paxillin in glomeruli from diabetic rats (in Japanese). Nippon Jinzo Gakkai Shi 38: 57-64.

    PubMed  CAS  Google Scholar 

  • Shimizu Y, Van Seventer GA, Horgan KJ and Shaw S (1990) Regulated expression and binding of three VLA (β2) integrin receptor on T cells. Nature 345: 250-253.

    Article  PubMed  CAS  Google Scholar 

  • Sinnet-smith J, Zachary I, Valverde AM and Rozengurt E (1993) Bombesin stimulation of p125 focal adhesion kinase tyrosine phosphorylation. J Biol Chem 268: 14,261-14,268.

    Google Scholar 

  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzana MD, Fuzimato EK, Goeke NM, Olson BJ and Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85.

    Article  PubMed  CAS  Google Scholar 

  • Stossel TP (1993) On the crawling of animal cells. Science 260: 1086-1094.

    PubMed  CAS  Google Scholar 

  • Taverna D, Disatnik M-H, Rayburn H, Branson RT, Yang J, Rando TA and Hynes RO (1998) Dystrophic muscle in mice chimeric for expression of α5 integrin. J Cell Biol 143: 849-859.

    Article  PubMed  CAS  Google Scholar 

  • Vachon PH, Xu H, Liu L, Loechel F, Hayashi Y, Arahata K, Reed JC, Wewer UM and Engvall E (1997) Integrin (α7 α1) in muscle function and survival. Disrupted expression in merosin-defficient congenital muscular dystrophy. J Clin Invest 100: 1870-1881.

    Article  PubMed  CAS  Google Scholar 

  • Vuori K and Ruoslahti E (1993) Activation of Protein Kinase C procedes α5β1 integrin-mediated cell spreading on fibronectin. J Biol Chem 268: 21,459-21,462.

    CAS  Google Scholar 

  • Vuori K and Ruoslahti E (1994) Association of insulin receptor substrate-1 with integrins. Science 266: 1576-1578.

    PubMed  CAS  Google Scholar 

  • Woods A and Couchman JR (1992) Protein kinase C involvement in focal adhesion formation. J Cell Sci 101: 277-290.

    PubMed  CAS  Google Scholar 

  • Yamboliev IA, Chen J and Gerthoffer WT (2001) PI 3-kinase and Src kinases regulate spreading and migration of cultured VSMCs. Am J Physiol Cell Physiol 281: C709-C718.

    PubMed  CAS  Google Scholar 

  • Zachary I, Sinnet-smith J, Turner CE and Rozengurt E (1993) Bombesin, vasopressin and endothelin rapidly stimulate tyrosine phosphorylation of the focal adhesion associated protein paxillin in Swiss 3T3 cells. J Biol Chem 268: 22,060-22,065.

    CAS  Google Scholar 

  • Zhang C, Qui HE, Krafft GA and Klein WL (1996) Protein kinase C and F-actin are essential for stimulation of neuronal FAK tyrosine phosphorylation by G-proteins and amyloid beta protein. FEBS Lett 386: 185-188.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Signal Transduction Research Laboratory, Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, Punjab, 160 062, India

    Hira Lal Goel & Chinmoy Sankar Dey

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  1. Hira Lal Goel
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  2. Chinmoy Sankar Dey
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Goel, H.L., Dey, C.S. Role of protein kinase C during insulin mediated skeletal muscle cell spreading. J Muscle Res Cell Motil 23, 269–277 (2002). https://doi.org/10.1023/A:1022028808806

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