Abstract
Dragonflies belong to the group of organisms with numerous well-differentiated species-specific characters at the adult stage, on the one hand, and a significantly smaller number or even the absence of such characters at the early ontogenetic stages. An example of the genus Aeschna is used to show difficulties in revealing morphological and biochemical characters allowing identification of larval dragonflies belonging to closely related species of the family. Distinct morphometric characters can be found only in late-instar larvae. The presence of species-specific proteins in the homogenates of thoracic muscles provides the possibility of using biochemical tests for species identification of larvae. Infestation by parasites has no effects on the biochemical parameters studied. Species identification of the early-instar dragonfly larvae is still problematic.
Similar content being viewed by others
REFERENCES
Belyakova, N.A. and Lukhtanov, V.A., Karyotypic Features of Some Lepidopteran (Lepidoptera) Families Belonging to the Noctuid and Bombycid Complexes, Entomol. Obozr., 1994, vol. 73, no.3, pp. 723–738.
Belyshev, B.F., Strekozy Sibiri (Siberian Dragonflies), Novosibirsk: Nauka SO, 1973, vol. 1.
Carchini, G. A., Key to the Italian Odonate Larvae, Utrecht, 1983.
Carius, W., Lakomy, W., Schuderer, R., and West, H., Verwandtschaftliche Bezienhungen Der Europaischen Vertreter der Gattung Aeschnidae. Biochemische und morphologische Merkmale der Flugmuskulatur und der Flugel: Vortr. Entomologentag., Jena, 23–27 Mars, 1993, Mitt. Dtsch. Ges. Allg. und Angew. Entomol., 1995, vol. 9, nos.4–6, p. 799.
Conn, J.E. and O'Brien, K.B., Molecular Systematics and Population Genetics of Polynesian Black Flies: Preliminary Mitochondrial DNA Data, Proc. 20th Int. Congr. Entomol., Firenze, Aug. 25–31, 1996, Firenze, 1996, p. 24.
Dolgikh, I.I., Grigor'ev, V.M., Sokolova, Yu.Y., and Issi, I.V., Effect of Invasion by Nosema grylli and Adelina sp. on Lactate Dehydrogenase Isozyme Composition and Activity in the Fat Body of Crickets Gryllus bimaculatus, Parazitologiya, 1995, vol. 29, no.6, pp. 520–525.
Gunderina, N.I., Kiknadze, I.I., and Bogachev, S.S., Comparative Analysis of Salivary Gland Secretion Proteins in Chironomid Larvae, 1 Vses. konf. po genet. nasekomykh, Moskva, 19–21 noyabrya 1991g. Tez. dokl. (Abstr. 1st All-Union Conf. on Insect Genetics, November 19–21, 1991), Moscow, 1991, p. 36.
Hoppe, K.L., Dillwith, J.W., Wright, R.E., and Szumlas, ?D.E., Identification of Horse Flies (Diptera; Tabanidae) by Analysis of Cuticular Hydrocarbons, J. Med. Entomol., 1990, vol. 27, no.4, pp. 480–486.
Kaib, M., Brandl, R., and Bagine, R.K.N., Cuticular Hydrocarbon Profiles: A Valuable Tool in Termite Taxonomy, Naturwissenschaften (Berlin), 1991, vol. 78, no.4, pp. 176–193.
Kharitonov, A.Yu., Dragonflies, in Opredelitel' presnovodnykh bespozvonochnykh Rossii i sopredel'nykh territorii (A Key to Freshwater Invertebrates of Russia and Neighboring Areas), vol. 3: Paukoobraznye i nizshie nasekomye (Arachnids and Lower Insects), St. Petersburg: Nauka, 1997, p. 222.
Khuda-Bukhsh, A.R. and Sen, S., Nonspecific Esterase in Three Species of Aphids (Homoptera: Aphididae), Nat. Acad. Sci. Lett, 1990, vol. 13, no.10, pp. 395–401.
Kiknadze, I.I., Gunderina, L.I., Kerkis, I.E., and Filippova, ?M.A., Cytogenetic Analysis of Chironomids as a Basis for Estimating Their Role as Bioindicators of Water Purity and Anthropogenic Pollution of Water Bodies, in Genetika—narodnomu khozyaistvu: Inf. mater. (Genetics in Service to People's Economy: Information), Novosibirsk, 1990, p. 96.
Laemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature (London), 1970, vol. 227, p. 680.
Lapenta, A.S., De Campos, B., Ceron, C.R., and Cordiero, J.A., Esterase Patterns of Species in the Drosophila buzzatii Cluster, Cytobios, 1995, vol. 84, no.336, pp. 13–29.
Mangium, S. and Hung, A.C.F., Developmental Genetics in Larvae, Pupae and Adults of Sepedon fuscipennis fuscipennis (Diptera: Sciomyzidae), Entomophaga, 1991, vol. 36, no.2, pp. 183–197.
Murray, R.A. and Solomon, M.G., A Rapid Technique for Analyzing Diets of Invertebrate Predators by Electrophoresis, Ann. Appl. Biol., 1978, vol. 90, pp. 7–14.
Popova, A.N., Lichinki strekoz fauny SSSR (Odonata) (Odonate Larvae in the USSR Fauna), Moscow: Akad. Nauk SSSR, 1953.
Su Zhi-Hui, Ohama, T., and Okada, T., Phylogenetic Relationship and Evolution of the Japanese Carabinae Ground Beetles Based on Mitochondrial ND5 Sequences, J. Mol. Evol., 1996, vol. 42, no.2, pp. 124–129.
Tillyard, R.J., The Biology of Dragonflies (Odonata or Paraneuroptera), Cambridge: Cambridge Univ. Press, 1917.
Turak, E. and Hales, D.F., Sitobion: Genetics and the Environment, Acta Phytopathol. Entomol. Hung., 1990, vol. 25, nos.1–4, pp. 307–319.
Walton, M.P., Loxdale, H.D., and Williams, L.A., Electroforetic “Keys” for the Identification of Parasitoids (Homoptera: Aphidodea), Biol. J. Linn. Soc., 1990, vol. 40, no.4, pp. 333–348.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sukhacheva, G.A., Kryukova, N.A. & Glupov, V.V. On the Roles of Morphological and Biochemical Criteria in Species Identification: An Example of Dragonfly Larvae of the Genus Aeschna . Biology Bulletin 30, 63–68 (2003). https://doi.org/10.1023/A:1022019611654
Issue Date:
DOI: https://doi.org/10.1023/A:1022019611654