Skip to main content
SpringerLink
Log in

Use of Nested PCR for Differential Diagnosis of Falciparum Malaria Reinfection and Relapse in Drug-Resistant Patients

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Microscopic examination does not allow differentiation of drug-resistant P. falciparum infection relapse from reinfection. However, this differential diagnosis is essential for adequate therapy. Three highly polymorphic P. falciparum genes (msp1, msp2, and glurp) and their alleles reflecting the structural state of these genes were used as genetic markers for differential diagnosis by PCR with internal primers. In 27 patients the characteristics of these alleles were identical before treatment with artersunate and during repeated manifestation of symptoms 14-28 days after the end of therapy, which attested to malaria relapses. In 24 patients the structure of these allele before mefloquine therapy and during repeated manifestation of the symptoms after 2-3 months was different, which attested to reinfection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Nguen Van Ha, Le Dyk Dao, and S. A. Rabinovich, Biomed. Tekhnol., No. 16, 153–163 (2001).

  2. H. Contamin, T. Fandeur, C. Rogier, et al., Am. J. Trop. Med. Hyg., 54,No. 6, 632–634 (1996).

    Google Scholar 

  3. D. J. Conway and J. S. McBride, Biochem. Parasitol., 103, 7–16 (1992).

    Google Scholar 

  4. I. Felger, L. Tavul, S. Kabintik, et al., Exp. Parasitol., 79,No.2, 106–116 (1994).

    Google Scholar 

  5. F. Ntoumi, H. Contamin, C. Rogier, et al., Am. J. Trop. Med. Hyg., 52,No. 1, 81–88 (1995).

    Google Scholar 

  6. G. Snounou and H. P. Beck, Parasitol. Today, 14,No. 11, 462–467 (1998).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Malariology, Parasitology, and Entomology, Hanoi

    Nguen Van Ha, Le Dyk Dao & S. A. Rabinovich

  2. Medical Faculty, Russian University of Peoples' Friendship, Russia

    Nguen Van Ha, Le Dyk Dao & S. A. Rabinovich

  3. E. I. Martsinovskii Institute of Medical Parasitology and Tropical Medicine, Moscow

    Nguen Van Ha, Le Dyk Dao & S. A. Rabinovich

Authors
  1. Nguen Van Ha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Le Dyk Dao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. A. Rabinovich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Van Ha, N., Dyk Dao, L. & Rabinovich, S.A. Use of Nested PCR for Differential Diagnosis of Falciparum Malaria Reinfection and Relapse in Drug-Resistant Patients. Bulletin of Experimental Biology and Medicine 134, 379–381 (2002). https://doi.org/10.1023/A:1021968517124

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1021968517124

Search

Navigation