Abstract
Strains of Rhizobium spp. isolated from leguminous plants and standard strains accumulated 27% to 57% polyhydroxyalkanoate (PHA) of their cell biomass. Among these cultures, one strain of Rhizobium meliloti synthesized 10–30% more PHA than others and contained 3% hydroxyvalerate (HV) when grown on sucrose as carbon substrate. The occurrence of hydroxybutyrate (HB) and HV was confirmed by GC and 1H NMR analysis. Treatment of the culture with 4′-N-piperidinobutyl-2-chlorophenoxazine resulted in a mutant which synthesized upto 69%, PHA of the cell biomass with an improved yield of 11 to 47% under different carbon and nitrogen ratios, compared to the parent strain.
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References
Akiyama M, Doi Y (1993) Production of poly(3-hydroxyalkanoates) from β,β-alkanedoic acids and hydroxylated fatty acids by Alcaligenes sp. Biotechnol. Lett. 15: 163–168.
Anderson AJ, Dawes EA (1990) Occurrence, metabolism, metabolic role, and industrial uses of polyhydroxyalkanoates. Microbiol. Rev. 54: 450–472.
Brandl H, Gross RA, Lenz RW, Fuller RC (1988) Pseudomonas oleovorans as a source of poly-β-(HA) for potential application as biodegradable polyesters. Appl. Environ. Microbiol. 154: 1977–1982.
Fouchet P, Jan S, Courtois J, Courtois B, Frelat G, Barbotin JN (1995) Quantitative single cell detection of poly(β-hydroxybutyrate) accumulation in Rhizobium meliloti by flow cytometry. FEMS Microbiol. Lett. 126: 31–36.
Huisman GW, Wonick E, de Koning G, Preusting H, Witholt B (1992) Synthesis of poly(3-hydroxyalkanoates) by mutant and recombinant Pseudomonas strains. Appl. Microbiol. Biotechnol. 38: 1–5.
Labuzek S, Radecka I (2001) Biosynthesis of PHB tercopolymer by Bacillus cereus UW 85. J. Appl. Microbiol. 90: 353–357.
Law JH, Slepecky RA (1961) Assay of polyhydroxybutyric acid. J. Bacteriol. 82: 33–36.
Madison L, Huisman GW (1999) Metabolic engineering of poly(3-hydroxyalkanoates: from DNA to plastics. Microbiol. Mol. Biol. Rev. 63: 21–53.
Martinez P, Guzman J, Espin G (1997) A mutation impairing alginate production increased accumulation of poly-β-hydroxybutyrate in Azotobacter vinelandii. Biotechnol. Lett. 19: 909–912.
Page WJ, Manchak J, Rudy B (1992) Formation of poly(hydroxybutyrate-co-valerate) by Azotobacter vinelandii UWD. Appl. Environ. Microbiol. 58: 2866–2873.
Rodrigues MFA, de Silva LF, Gomes JGC, Valentin HE, Steinbuchel A (1995) Biosynthesis of poly(3-hydroxybutyric acidco-3-hydroxy-4-pentanoic acid) from unrelated substrates by Burkholderia sp. Appl. Microbiol. Biotechnol. 43: 880–886.
Solaiman DKY, Ashby RD, Foglia TA (2001) Production of polyhydroxyalkanoates from intact triacylglycerol by genetically engineered Pseudomonas. Appl. Microbiol. Biotechnol. 56: 664–669.
Takahashi H, Miyake M, Tokiwa Y, Asada Y (1998) Improved accumulation of poly 3-hydroxybutyrate by a recombinant cyanobacterium. Biotechnol. Lett. 20: 183–186.
Tombolini R, Nuti MP (1989) Poly(β-hydroxyalkanoate) biosynthesis and accumulation by different Rhizobium species. FEMS Microbiol. Lett. 60: 299–304.
Vincent JM, Nutman PS, Skinner FA (1968) The identification and classification of Rhizobium. In: Skinner FA, Lovelock DW, eds. Identification Methods for Microbiologists. New York: Academic Press, pp. 49–69.
Williamson DH, Wilkinson JF (1958) The isolation and estimation of poly-β-hydroxybutyrate inclusions of Bacillus species. J. Gen. Microbiol. 19: 198–209.
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Lakshman, K., Ramachandriah Shamala, T. Enhanced biosynthesis of polyhydroxyalkanoates in a mutant strain of Rhizobium meliloti . Biotechnology Letters 25, 115–119 (2003). https://doi.org/10.1023/A:1021959100432
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DOI: https://doi.org/10.1023/A:1021959100432