Abstract
Gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, was expressed in Escherichia coli using expression vector pET-32a(+). The gene was expressed under T7 promotor with a fusion partner of Thx.Tag and a 6xHis.Tag at its 5′ terminal. After induction by IPTG for 6 h, the recombinant enzyme was expressed in the cytoplasm. Expression at 25 °C gave twice the amount of recombinant gloshedobin in cytoplasm than at 37 °C.
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Yang, Q., Li, M., Xu, J. et al. Expression of gloshedobin, a thrombin-like enzyme from the venom of Gloydius shedaoensis, in Escherichia coli . Biotechnology Letters 25, 101–104 (2003). https://doi.org/10.1023/A:1021950815453
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DOI: https://doi.org/10.1023/A:1021950815453