Cancer Causes & Control

, Volume 13, Issue 10, pp 929–935 | Cite as

Effect of diet and exercise on serum insulin, IGF-I, and IGFBP-1 levels and growth of LNCaP cells in vitro (United States)

  • Tung H. Ngo
  • R. James Barnard
  • Christopher N. Tymchuk
  • Pinchas Cohen
  • William J. Aronson


Objective: Accumulating evidence indicates that prostate cancer is associated with high levels of serum IGF-I. This study was conducted to determine whether a low-fat diet and exercise (DE) intervention may modulate the IGF axis and reduce prostate cancer cell growth in vitro. Methods: Fasting serum was obtained from 14 men (age 60 ± 3 years) participating in an 11-day DE program and from eight similarly aged men who had followed the DE program for 14.2 ± 1.7 years (long-term). Insulin, IGF-I, IGFBP-1, and IGFBP-3 were measured by ELISA, and serum was used to stimulate LNCaP cell growth in vitro. Results: Serum IGF-I levels decreased by 20% while IGFBP-1 increased by 53% after 11-day DE. In the long-term group, IGF-I was 55% lower, while IGFBP-1 was 150% higher relative to baseline. Serum insulin decreased by 25% after 11-day DE and was 68% lower in the long-term group, relative to baseline. No changes in serum IGFBP-3 were observed. Serum-stimulated LNCaP cell growth was reduced by 30% in post-11-day serum and by 44% in long-term serum relative to baseline. LNCaP cells incubated with post-DE serum showed increased apoptosis/necrosis, compared to baseline. Conclusions: A low-fat diet and exercise intervention induces in-vivo changes in the circulating IGF axis and is associated with reduced growth and enhanced apoptosis/necrosis of LNCaP tumor cells in vitro.

apoptosis dietary fat IGF-I axis physical activity prostate cancer 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Tung H. Ngo
    • 1
  • R. James Barnard
    • 1
    • 2
    • 3
  • Christopher N. Tymchuk
    • 1
  • Pinchas Cohen
    • 4
  • William J. Aronson
    • 5
  1. 1.Departments of Physiological ScienceUniversity of CaliforniaLos AngelesUSA
  2. 2.Department of Physiological ScienceUniversity of CaliforniaLos Angeles
  3. 3.Los AngelesUSA
  4. 4.PediatricsUniversity of CaliforniaLos AngelesUSA
  5. 5.UrologyUniversity of CaliforniaLos AngelesUSA

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