Skip to main content

Dynamic Light Scattering and NMR Studies of Napin

Abstract

We analyze the structure of napin (BngNAP1), a storage protein (m.w. 14.5 kDa) from Brassica napus. On the basis of the results of 1H NMR spectroscopy and dynamic light scattering (DLS) studies, the overall shape and secondary structure of the molecule are estimated.

This is a preview of subscription content, access via your institution.

References

  1. M. L. Crouch, K. M. Tenbarge, A. E. Simon, and R. Ferl, J. Mol. Appl. Gen. 2, 273(1983).

    Google Scholar 

  2. T. J. V. Higgins, Annu. Rev. Plant Physiol. 35, 191(1984).

    Google Scholar 

  3. M. L. Ericson, E. Muren, H-O. Gustavsson, L. G. Josefsson, and L. Rask, Eur. J. Biochem. 197, 741(1991).

    Google Scholar 

  4. R. I. Monsalve and R. Rodriguez, J. Exp. Bot. 41, 89(1990).

    Google Scholar 

  5. A. Byczyńska and J. Barciszewski, J. Plant Physiol. 154, 417(1999)

    Google Scholar 

  6. M. L. Ericson, J. Rödin, M. Lenman, K. Glimelius, L.-G. Josefsson, and L. Rask, J. Biol. Chem. 261, 14576(1986).

    Google Scholar 

  7. P. M. Gehrig and K. Biemann, Peptide Res. 9, 308(1996).

    Google Scholar 

  8. R. I. Monsalve, C. Lopez-Otin, M. Villalba, and R. Rodriguez, FEBS Lett. 295, 207(1991).

    Google Scholar 

  9. E. Krebbers, L. Herdies, A. DeClercq, J. Seurinck, J. Leemans, J. VanDamme, M. Segura, G. Gheysen, M. Van Montagu, and J. Vandekerckhove, Plant Physiol. 87, 859(1988).

    Google Scholar 

  10. Ch. Ampe, J. Van Damme, L. A. B. de Castro, M. J. A. M. Sampaos, M. Van Montagu, and J. Vandekerckhove, Eur. J. Biochem 159, 597(1986).

    Google Scholar 

  11. E. Murén, B. Ek, and L. Rask, Eur. J. Biochem. 242, 214(1996).

    Google Scholar 

  12. A. Krzyzaniak, T. Burova, T. Haertle, and J. Barciszewski, Nahrung 42, 201(1997).

    Google Scholar 

  13. R. I. Monsalve, L. Menendez-Arias, C. Lopez-Otin, and R. Rodriguez, FEBS Lett. 263, 209(1990).

    Google Scholar 

  14. R. I. Monsalve, M. Villalba, C. Lopez-Otin, and R. Rodriguez, Biophys. Biochim. Acta 1078, 265(1991).

    Google Scholar 

  15. M. Rico, M. Bruix, C. Gonzalez, R. I. Monsalve, and R. Rodriguez, Biochemistry 35, 15672(1996).

    Google Scholar 

  16. M. Rance, O. W. Soerensen, G. Bodenhausen, G. Wagner, R. R. Ernst, and K. Wüthrch, Biochem. Biophys. Res. Commun. 117, 579(1983).

    Google Scholar 

  17. D. Marion and K. Wüthrich, Biochem. Biophys. Res. Commun. 113, 967(1983).

    Google Scholar 

  18. Ch. R. Canto and P. R. Schimmel, Biophysical Chemistry, (Freeman, San Francisco, CA, 1980).

    Google Scholar 

  19. D. S. Wishart, B. D. Sykes, and F. M. Richards, FEBS Lett. 293, 72(1991).

    Google Scholar 

  20. I. D. Kuntz and W. Kauzmann, Advan. Protein. Chem. 28, 239(1997).

    Google Scholar 

  21. D. I. Svergun, S. Richards, M. H. J. Koch, Z. Sayers, S. Kuprin, and G. Zaccai, Proc. Natl. Acad. Sci. U.S. 95, 2267(1998).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Genowefa Ślósarek.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kaszyńska, K., Banachowicz, E., Ślósarek, G. et al. Dynamic Light Scattering and NMR Studies of Napin. Journal of Solution Chemistry 31, 987–993 (2002). https://doi.org/10.1023/A:1021877526536

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1021877526536

  • Napin
  • dynamic light scattering
  • hydrodynamic properties
  • secondary structure