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Variable Tandem Repeat VcA of Vibrio cholerae

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Abstract

Computer analysis revealed seven potential variable-number tandem-repeat (VNTR) loci in the Vibrio cholerae genome. Specific primers were designed to amplify locus VcA located on chromosome 2 and containing a TGCTGT repeat. The locus was found in all tested strains from aV. cholerae strain collection, the repeat number varying from 3 to 23. In total, 14 VcA alleles were observed. The VcA locus was proposed as a marker for the molecular typing of V. cholerae strains.

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REFERENCES

  1. Welsh, J. and McClelland, M., Nucleic Acids Res., 1990, vol. 18, pp. 7213–7218.

    Google Scholar 

  2. Majumder, R., Sengupta, S., Khetawat, G., et al., J. Bacteriol., 1996, vol. 56, pp. 1105–1112.

    Google Scholar 

  3. Janssen, P., Coopman, R., Huys, G., et al., Microbiology, 1996, vol. 142, pp. 1881–1893.

    Google Scholar 

  4. Rivera, J.G., Chowdhury, M.A.R., Huq, A., et al., Appl. Environ. Microbiol., 1995, vol. 61, pp. 2898–2904.

    Google Scholar 

  5. Martin, B., Humbert, O., Camara, M., et al., Nucleic Acids Res., 1992, vol. 20, pp. 3479–3483.

    Google Scholar 

  6. van Belkum, A., Scherer, S., van Alphen, Z., and Verbrugh, H., Microbiol. Mol. Biol. Rev., 1998, vol. 62, pp. 275–293.

    Google Scholar 

  7. Haber, J.E. and Louis, E.J., Genomics, 1998, vol. 15, pp. 132–135.

    Google Scholar 

  8. Nakamura, Y., Leppert, M., O'Connell, P., et al., Science, 1987, vol. 235, pp. 1616–1622.

    Google Scholar 

  9. Efremov, I.A., Chistyakov, D.A., and Nosikov, V.V., Mol. Biol., 1996, vol. 30, pp. 307–318.

    Google Scholar 

  10. Klevytska, A.M., Price, L.B., Schupp, J.M., et al., J. Clin. Microbiol., 2001, vol. 39, pp. 3179–3185.

    Google Scholar 

  11. Le Flèche, P., Hauck, Y., Onteniente, L., et al., BMC Microbiology, 2001, vol. 1, p. 2.

    Google Scholar 

  12. Adair, D.M., Worsham, P.L., Hill, K.K., et al., J. Clin. Microbiol., 2000, vol. 38, pp. 1516–1519.

    Google Scholar 

  13. van Belkum, A., Scherer, S., van Leeuwen, W., et al., Infect. Immunol., 1997, vol. 65, pp. 5017–5025.

    Google Scholar 

  14. Johansson, A., Goransson, I., Larsson, P., and Sjostedt, A., J. Clin. Microbiol., 2001, vol. 39, pp. 3140–3146.

    Google Scholar 

  15. Heidelberg, J.F., Eisen, J.A., Nelson, W.C., et al., Nature, 2000, vol. 406, pp. 477–483.

    Google Scholar 

  16. Vodopyanov, S.O., Mishan'kin, M.B., Oleinikov, I.P., et al., Biotekhnologiya, 1999, vol. 6, pp. 19–23.

    Google Scholar 

  17. Vodopyanov, A.S., Vodopyanov, S.O., Mishan'kin, B.N., and Suchkov, I.Yu., Biotekhnologiya, 2001, vol. 6, pp. 85–88.

    Google Scholar 

  18. Johnson, J.A., Salles, C.A., Pinigrani, P., et al., Infect. Immunol., 1994, vol. 62, pp. 2108–2110.

    Google Scholar 

  19. Mishan'kin, B.N., Romanova, L.V., Lomov, Yu.M., et al., Zh. Epidemiol. Mikrobiol. Immunobiol., 2000, vol. 3, pp. 3–7.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Antiplague Research, Rostov-on-Don, 344007, Russia

    S. O. Vodopyanov, I. P. Oleinikov, E. K. Goncharov, O. V. Duvanova, M. B. Mishan'kin, I. Yu. Suchkov, A. S. Vodopyanov, M. V. Shishiyanu, E. V. Shcherbakova, I. V. Kornienko & B. N. Mishan'kin

  2. 124 Central Laboratory of Forensic Identification, Ministry of Defense of the Russian Federation, Rostov-on-Don, 344010, Russia

    S. O. Vodopyanov, I. P. Oleinikov, E. K. Goncharov, O. V. Duvanova, M. B. Mishan'kin, I. Yu. Suchkov, A. S. Vodopyanov, M. V. Shishiyanu, E. V. Shcherbakova, I. V. Kornienko & B. N. Mishan'kin

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  1. S. O. Vodopyanov
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  2. I. P. Oleinikov
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  3. E. K. Goncharov
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  4. O. V. Duvanova
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  5. M. B. Mishan'kin
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  6. I. Yu. Suchkov
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  7. A. S. Vodopyanov
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  8. M. V. Shishiyanu
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  9. E. V. Shcherbakova
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  10. I. V. Kornienko
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  11. B. N. Mishan'kin
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Vodopyanov, S.O., Oleinikov, I.P., Goncharov, E.K. et al. Variable Tandem Repeat VcA of Vibrio cholerae. Molecular Biology 36, 874–878 (2002). https://doi.org/10.1023/A:1021650429863

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  • DOI: https://doi.org/10.1023/A:1021650429863

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